Functional complementation of UvsX and UvsY mutations in the mediation of T4 homologous recombination

Nucleic Acids Research

Volumn 37, Issue 7, 2009, Pages 2336-2345

  Farb, Joshua N ^a   Morrical, Scott W ^a  

^a UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN UVSX; PROTEIN UVSY; RECOMBINASE; SINGLE STRANDED DNA; UNCLASSIFIED DRUG;

ARTICLE; BACTERIOPHAGE T4; BINDING AFFINITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; GENE MUTATION; GENETIC COMPLEMENTATION; HOMOLOGOUS RECOMBINATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN FUNCTION; VIRAL GENETICS; VIRUS MUTANT; WILD TYPE;

ADENOSINE TRIPHOSPHATASES; AMINO ACID MOTIFS; BACTERIOPHAGE T4; DNA, SINGLE-STRANDED; DNA-BINDING PROTEINS; GENETIC COMPLEMENTATION TEST; MEMBRANE PROTEINS; MUTATION; REC A RECOMBINASES; RECOMBINATION, GENETIC; VIRAL PROTEINS;

EID: 65549108979     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkp096     Document Type: Article

References (33)

1. Bianco,P.R., Tracy,R.B. and Kowalczykowski,S.C. (1998) DNA strand exchange proteins: A biochemical and physical comparison. Front. Biosci., 3, D570-D603.
2. Yu,V.P., Koehler,M., Steinlein,C., Schmid,M., Hanakahi,L.A., van Gool,A.J., West,S.C. and Venkitaraman,A.R. (2000) Gross chromosomal rearrangements and genetic exchange between nonhomologous chromosomes following BRCA2 inactivation. Genes Dev., 14, 1400-1406.
3. Khanna,K.K. and Jackson,S.P. (2001) DNA double-strand breaks: Signaling, repair and the cancer connection. Nat. Genet., 27, 247-254.
4. Moynahan,M.E., Pierce,A.J. and Jasin,M. (2001) BRCA2 is required for homology-directed repair of chromosomal breaks. Mol. Cell, 7 263-272.
5. Jasin,M. (2002) Homologous repair of DNA damage and tumorigenesis: The BRCA connection. Oncogene, 21, 8981-8993.
6. Beernink,H.T. and Morrical,S.W. (1999) RMPs: Recombination/replication mediator proteins. Trends Biochem. Sci., 24, 385-389.
7. Sugiyama,T. and Kowalczykowski,S.C. (2002) Rad52 protein associates with replication protein A (RPA)-single-stranded DNA to accelerate Rad51-mediated displacement of RPA and presynaptic complex formation. J. Biol. Chem., 277, 31663-31672.
8. Liu,J., Qian,N. and Morrical,S.W. (2006) Dynamics of bacterio-phage T4 presynaptic filament assembly from extrinsic fluorescence measurements of Gp32-single-stranded DNA interactions. J. Biol. Chem., 281 26308-26319.
9. Bleuit,J.S., Xu,H., Ma,Y., Wang,T., Liu,J. and Morrical,S.W. (2001) Mediator proteins orchestrate enzyme-ssDNA assembly during T4 recombination-dependent DNA replication and repair. Proc. Natl Acad. Sci. USA, 98, 8298-8305.
10. Formosa,T. and Alberts,B.M. (1986) Purification and characterization of the T4 bacteriophage uvsX protein. J. Biol. Chem., 261, 6107-6118.
11. Farb,J.N. and Morrical,S.W. (2009) Role of allosteric switch residue histidine-195 in maintaining active site asymmetry in presynaptic filaments of bacteriophage T4 UvsX recombinase. J. Mol. Biol., 385, 393-404.
12. Cunningham,R.P. and Berger,H. (1977) Mutations affecting genetic recombination in bacteriophage T4D. I. Pathway analysis. Virology, 80, 67-82.
13. Melamede,R.J. and Wallace,S.S. (1977) Properties of the nonlethal recombinational repair x and y mutants of bacteriophage T4. II. DNA synthesis. J. Virol., 24, 28-40.
14. Melamede,R.J. and Wallace,S.S. (1980) Phenotypic differences among the alleles of the T4 recombination defective mutants. Mol. Gen. Genet. 179, 327-330.
15. Melamede,R.J. and Wallace,S.S. (1980) Properties of the nonlethal recombinational repair deficient mutants of bacteriophage T4. III. DNA replicative intermediates and T4w. Mol. Gen. Genet., 177, 501-509.
16. Sweezy,M.A. and Morrical,S.W. (1999) Biochemical interactions within a ternary complex of the bacteriophage T4 recombination proteins uvsY and gp32 bound to single-stranded DNA. Biochemistry, 38, 936-944.
17. Beernink,H.T. and Morrical,S.W. (1998) The uvsY recombination protein of bacteriophage T4 forms hexamers in the presence and absence of single-stranded DNA. Biochemistry, 37, 5673-5681.
18. Liu,J., Bond,J.P. and Morrical,S.W. (2006) Mechanism of presynaptic filament stabilization by the bacteriophage T4 UvsY recombination mediator protein. Biochemistry, 45, 5493-5502.
19. Bleuit,J.S., Ma,Y., Munro,J. and Morrical,S.W. (2004) Mutations in a conserved motif inhibit single-stranded DNA binding and recombination mediator activities of bacteriophage T4 UvsY protein. J. Biol. Chem. 279, 6077-6086.
20. Pant,K., Shokri,L., Karpel,R.L., Morrical,S.W. and Williams,M.C. (2008) Modulation of T4 gene 32 protein DNA binding activity by the recombination mediator protein UvsY. J. Mol. Biol., 380, 799-811.
21. Sambrook,J., Fritsch,E.F. and Maniatis,T. (1989) Molecular cloning: A laboratory manual. 2nd edn. Cold Spring Harbor Laboratory Press, Plainview, NY.
22. Ando,R.A. and Morrical,S.W. (1998) Single-stranded DNA binding properties of the UvsX recombinase of bacteriophage T4: Binding parameters and effects of nucleotides. J. Mol. Biol., 283, 785-796.
23. Sweezy,M.A. and Morrical,S.W. (1997) Single-stranded DNA binding properties of the uvsY recombination protein of bacteriophage T4. J. Mol. Biol., 266, 927-938.
24. Gill,S.C. and von Hippel,P.H. (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal. Biochem., 182 319-326.
25. Yassa,D.S., Chou,K.M. and Morrical,S.W. (1997) Characterization of an amino-terminal fragment of the bacteriophage T4 uvsY recombination protein. Biochimie, 79, 275-285.
26. Morrical,S.W. and Alberts,B.M. (1990) The UvsY protein of bacteriophage T4 modulates recombination-dependent DNA synthesis in vitro. J. Biol. Chem., 265, 15096-15103.
27. Kodadek,T., Wong,M.L. and Alberts,B.M. (1988) The mechanism of homologous DNA strand exchange catalyzed by the bacteriophage T4 uvsX and gene 32 proteins. J. Biol. Chem., 263, 9427-9436.
28. Kodadek,T., Gan,D.C. and Stemke-Hale,K. (1989) The phage T4 uvsY recombination protein stabilizes presynaptic filaments. J. Biol. Chem., 264, 16451-16457.
29. Jiang,H., Salinas,F. and Kodadek,T. (1997) The gene 32 single-stranded DNA-binding protein is not bound stably to the phage T4 presynaptic filament. Biochem. Biophys. Res. Commun., 231, 600-605.
30. Lopper,M., Boonsombat,R., Sandler,S.J. and Keck,J.L. (2007) A hand-off mechanism for primosome assembly in replication restart. Mol. Cell 26, 781-793.
31. Liu,Y., Prasad,R., Beard,W.A., Kedar,P.S., Hou,E.W., Shock,D.D. and Wilson,S.H. (2007) Coordination of steps in single-nucleotide base excision repair mediated by apurinic/apyrimidinic endonuclease 1 and DNA polymerase beta. J. Biol. Chem., 282, 13532-13541.
32. Hitomi,K., Iwai,S. and Tainer,J.A. (2007) The intricate structural chemistry of base excision repair machinery: Implications for DNA damage recognition, removal, and repair. DNA Repair (Amst), 6, 410-428.
33. Bugreev,D.V. and Mazin,A.V. (2004) Ca2+ activates human homologous recombination protein Rad51 by modulating its ATPase activity. Proc. Natl Acad. Sci. USA, 101, 9988-9993.