Cleavage of Escherichia coli cytotoxic necrotizing factor 1 is required for full biologic activity

Infection and Immunity

Volumn 77, Issue 5, 2009, Pages 1835-1841

  Knust, Zeynep ^a   Blumenthal, Britta ^b   Aktories, Klaus ^a   Schmidt, Gudula ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b UNIVERSITY OF FREIBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOTOXIC NECROTIZING FACTOR 1; SERINE PROTEINASE; BACTERIAL TOXIN; ESCHERICHIA COLI PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTROLLED STUDY; CYTOSOL; ENDOSOME; ESCHERICHIA COLI; HELA CELL; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN TRANSPORT; CELL LINE; CHEMISTRY; ENZYME ACTIVE SITE; HYDROLYSIS; METABOLISM; PATHOGENICITY; PH; PROTEIN TERTIARY STRUCTURE;

BACTERIAL TOXINS; CATALYTIC DOMAIN; CELL LINE; CYTOSOL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; PROTEIN STRUCTURE, TERTIARY;

EID: 65449144434     PISSN: 00199567     EISSN: 10985522     Source Type: Journal    
DOI: 10.1128/IAI.01145-08     Document Type: Article

References (20)

1. Blumenthal, B., C. Hoffmann, K. Aktories, S. Backert, and G. Schmidt. 2007. The cytotoxic necrotizing factors from Yersinia pseudotuberculosis and from Escherichia coli bind to different cellular receptors but take the same route to the cytosol. Infect. Immun. 75:3344-3353.
2. Boquet, P. 2001. The cytotoxic necrotizing factor 1 (CNF1) from Escherichia coli. Toxicon 39:1673-1680.
3. Buetow, L., G. Flatau, K. Chiu, P. Boquet, and P. Ghosh. 2001. Structure of the Rho-activating domain of Escherichia coli cytotoxic necrotizing factor 1. Nat. Struct. Biol. 8:584-588.
4. Caprioli, A., V. Falbo, F. M. Ruggeri, L. Baldassarri, R. Bisicchia, G. Ippolito, E. Romoli, and G. Donelli. 1987. Cytotoxic necrotizing factor production by hemolytic strains of Escherichia coli causing extraintestinal infections. J. Clin. Microbiol. 25:146-149.
5. Contamin, S., A. Galmiche, A. Doye, G. Flatau, A. Benmerah, and P. Boquet. 2000. The p21 Rho-activating toxin cytotoxic necrotizing factor 1 is endocytosed by a clathrin-independent mechanism and enters the cytosol by an acidic-dependent membrane translocation step. Mol. Biol. Cell 11:1775-1787.
6. Flatau, G., E. Lemichez, M. Gauthier, P. Chardin, S. Paris, C. Fiorentini, and P. Boquet. 1997. Toxin-induced activation of the G protein p21 Rho by deamidation of glutamine. Nature 387:729-733.
7. Gerhard, R., G. Schmidt, F. Hofmann, and K. Aktories. 1998. Activation of Rho GTPases by Escherichia coli cytotoxic necrotizing factor 1 increases intestinal permeability in Caco-2 cells. Infect. Immun. 66:5125-5131.
8. Hoffmann, C., M. Pop, J. Leemhuis, J. Schirmer, K. Aktories, and G. Schmidt. 2004. The Yersinia pseudotuberculosis cytotoxic necrotizing factor (CNFY) selectively activates RhoA. J. Biol. Chem. 279:16026-16032. (Pubitemid 38509292)
9. Hoffmann, C., and G. Schmidt. 2004. CNF and DNT. Rev. Physiol. Biochem. Pharmacol. 152:49-63.
10. Khan, N. A., Y. Wang, K. J. Kim, J. W. Chung, C. A. Wass, and K. S. Kim. 2002. Cytotoxic necrotizing factor-1 contributes to Escherichia coli K1 invasion of the central nervous system. J. Biol. Chem. 277:15607-15612.
11. Kim, K. J., J. W. Chung, and K. S. Kim. 2005. 67-kDa laminin receptor promotes internalization of cytotoxic necrotizing factor 1-expressing Escherichia coli K1 into human brain microvascular endothelial cells. J. Biol. Chem. 280:1360-1368.
12. Lemichez, E., G. Flatau, M. Bruzzone, P. Boquet, and M. Gauthier. 1997. Molecular localization of the Escherichia coli cytotoxic necrotizing factor CNF1 cell-binding and catalytic domains. Mol. Microbiol. 24:1061-1070.
13. Lemonnier, M., L. Landraud, and E. Lemichez. 2007. Rho GTPase-activating bacterial toxins: from bacterial virulence regulation to eukaryotic cell biology. FEMS Microbiol. Rev. 31:515-534.
14. Lerm, M., J. Selzer, A. Hoffmeyer, U. R. Rapp, K. Aktories, and G. Schmidt. 1999. Deamidation of Cdc42 and Rac by Escherichia coli cytotoxic necrotizing factor 1: activation of c-Jun N-terminal kinase in HeLa cells. Infect. Immun. 67:496-503.
15. Lockman, H. A., R. A. Gillespie, B. D. Baker, and E. Shakhnovich. 2002. Yersinia pseudotuberculosis produces a cytotoxic necrotizing factor. Infect. Immun. 70:2708-2714.
16. Meysick, K. C., M. Mills, and A. D. O'Brien. 2001. Epitope mapping of monoclonal antibodies capable of neutralizing cytotoxic necrotizing factor type 1 of uropathogenic Escherichia coli. Infect. Immun. 69:2066-2074. (Pubitemid 32239670)
17. Orden, J. A., G. Dominguez-Bernal, S. Martinez-Pulgarin, M. Blanco, J. E. Blanco, A. Mora, J. Blanco, J. Blanco, and R. de la Fuente. 2007. Necrotoxigenic Escherichia coli from sheep and goats produce a new type of cytotoxic necrotizing factor (CNF3) associated with the eae and ehxA genes. Int. Microbiol. 10:47-55. (Pubitemid 46556858)
18. Oswald, E., M. Sugai, A. Labigne, H. C. Wu, C. Fiorentini, P. Boquet, and A. D. O'Brien. 1994. Cytotoxic necrotizing factor type 2 produced by virulent Escherichia coli modifies the small GTP-binding proteins Rho involved in assembly of actin stress fibers. Proc. Natl. Acad. Sci. USA 91:3814-3818.
19. Pei, S., A. Doye, and P. Boquet. 2001. Mutation of specific acidic residues of the CNF1 T domain into lysine alters cell membrane translocation of the toxin. Mol. Microbiol. 41:1237-1247. (Pubitemid 32939498)
20. Schmidt, G., P. Sehr, M. Wilm, J. Selzer, M. Mann, and K. Aktories. 1997. Gln63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor 1. Nature 387:725-729.