Gin 63 of Rho is deamidated by Escherichia coli cytotoxic necrotizing factor-1

Nature

Volumn 387, Issue 6634, 1997, Pages 725-729

  Schmidt, Gudula ^a   Sehr, Peter ^a   Wilm, Matthias ^b   Selzer, Jörg ^a   Mann, Matthias ^b   Aktories, Klaus ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

^b EMBL   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN FILAMENT; ADENOSINE DIPHOSPHATE RIBOSYLATION; ARTICLE; CELL MIGRATION; CYTOSKELETON; CYTOTOXIC T LYMPHOCYTE; DEAMINATION; ENZYME ACTIVITY; ESCHERICHIA COLI; PRIORITY JOURNAL; PROTEIN ASSEMBLY; AMINO ACID SEQUENCE; ANIMAL; CELL STRAIN 3T3; CHEMISTRY; DRUG ANTAGONISM; DRUG EFFECT; GLYCOSYLATION; MASS SPECTROMETRY; METABOLISM; MICROINJECTION; MOLECULAR GENETICS; MOLECULAR WEIGHT; MOUSE; POLYACRYLAMIDE GEL ELECTROPHORESIS;

2'(3') O (N METHYL)ANTHRANILOYLGUANOSINE 5' TRIPHOSPHATE; 2'(3')-O-(N-METHYL)ANTHRANILOYLGUANOSINE 5'-TRIPHOSPHATE; ACTIN; ADENOSINE DIPHOSPHATE RIBOSE; ANTHRANILIC ACID DERIVATIVE; BACTERIAL TOXIN; CYTOTOXIC NECROTIZING FACTOR 1; CYTOTOXIN; ESCHERICHIA COLI PROTEIN; GLUTAMINE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATASE; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; GUANOSINE TRIPHOSPHATE; HYBRID PROTEIN; RHO GTPASE ACTIVATING PROTEIN; RHO GTPASE-ACTIVATING PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN;

3T3 CELLS; ACTINS; ADENOSINE DIPHOSPHATE RIBOSE; AMINO ACID SEQUENCE; ANIMALS; ANTHRANILIC ACIDS; BACTERIAL TOXINS; CYTOSKELETON; CYTOTOXINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GLUTAMINE; GLYCOSYLATION; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEINS; GTPASE-ACTIVATING PROTEINS; GUANOSINE TRIPHOSPHATE; MASS SPECTROMETRY; MICE; MICROINJECTIONS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; RECOMBINANT FUSION PROTEINS; RHOA GTP-BINDING PROTEIN;

EID: 0030610785     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/42735     Document Type: Article

References (22)

1. rho induces rapid changes in cell morphology. J. Cell Biol. 111, 1001-1007 (1990).
2. Machesky, L. M. & Hall, A. Rho: A connection between membrane receptor signalling and the cytoskeleton. Trends Cell Biol. 6, 304-310 (1996).
3. Lim, L., Manser, E., Leung, T. & Hall, C. Regulation of phosphorylation pathways by p21 GTPases the p21 Ras-related Rho subfamily and its role in phosphorylation signalling pathways. Eur. J. Biochem. 242, 171-185 (1996).
4. Just, I. et al. Glucosylation of Rho proteins by Clostridium difficile toxin B. Nature 375, 500-503 (1995).
5. Oswald, E. et al. Cytotoxic necrotizing factor type 2 produced by virulent Escherichia coli modifies the 'small GTP-binding proteins Rho involved in assembly of actin stress fibers. Proc. Natl Acad. Sci. USA 91, 3814-3818 (1994).
6. Fiorentini, C. et al. Escherichia coli cytotoxic necrotizing factor 1: Evidence for induction of actin assembly by constitutive activation of the p21 Rho GTPase. Infect. Immun. 63, 3936-3944 (1995).
7. Caprioli, A., Falbo, V., Roda, L. G., Ruggeri, F. M. & Zona, C. Partial purification and characterization of an Escherichia coli toxic factor that induces morphological cell alterations. Infect. Immun. 39, 1300-1306 (1983).
8. de Rycke, J. et al. Evidence for two types of cytotoxic necrotizing factor in human and animal clinical isolates of Escherichia coli. J. Clin. Microbiol. 28, 694-699 (1990).
9. Falzano, L. et al. Induction of phagocytic behaviour in human epithelial cells by Escherichia coli cytotoxic necrotizing factor type I. Mol. Microbiol. 9, 1247-1254 (1993).
10. Leonard, D. A., Evans, T., Hart, M., Cerione, R. A. & Manor, D. Investigation of the GTP-binding/ GTPase cycle of Cde42Hs using fluorescence spectroscopy. Biochemistry 33, 12323-12328 (1994).
11. Ridley, A. J. Microinjection of Rho and Rac into quiescent Swiss 3T3 cells. Methods Enzymol. 256, 313-320(1995).
12. Diekmann, D. & Hall, A. In virro binding assay for interactions of Rho and Rac with GTPase-activating proteins and effectors. Methods Enzymol 256, 207-215 (1995).
13. Renshaw, M. W., Toksoz, D. & Schwartz, M. A. Involvement of the small GTPase Rho in integrin-mediated activation of mitogen-activated protein kinase. J. Biol. Chem. 271, 21691-21694 (1996).
14. H genes mutated at codon 61. Cell 44, 167-176 (1986).
15. ras and other GTP-binding proteins. Nature Struct. Biol. 2, 36-44 (1995).
16. Just, I. et al. Clostridium difficile toxin B acts on the GTP-binding protein Rho. J. Biol. Chem. 269, 10706-10712 (1994).
17. Hiratsuka, T. New ribose-modified fluorescent analogs of adenine and guanine nucleotides available as substrates for various enzymes. Biochim. Biophys. Acta 742, 496-508 (1983).
18. Falbo, V., Pace, T, Picci, L., Pizzi, E. & Caprioli, A. Isolation and nucleotide sequence of the gene encoding cytotoxic necrotizing factor 1 of Escherichia coli. Infect. Immun. 61, 4909-4914 (1993).
19. Aktories, K. & Just, I. In vitro ADP-ribosylation of Rho by bacterial ADP-ribosyltransferases. Methods Enzymol. 256, 184-195 (1995).
20. Just, I., Selzer, J., Von Eichel-Streiber, C. & Aktories, K. The low molecular mass GTP-binding protein Rho is affected by toxin A from Clostridium difficile. J. Clin, Invest. 95, 1026-1031 (1995).
21. Horiguchi, Y., Senda, T., Sugimoto, N., Katahira, J. & Matsuda, M. Bordetella bronchiseptica dermonecrotizing toxin stimulates assembly of actin stress fibers and focal adhesions by modifying the small GTP-binding protein rho. J. Cell Sci. 108, 3243-3251 (1995).
22. Wilm, M. & Mann, M. Analytical properties of the nanoelectrospray ion source. Anal. Chem. 68, 1-8 (1996).