Location of functional region at N-terminus of polyhydroxyalkanoate (PHA) synthase by N-terminal mutation and its effects on PHA synthesis

Biochemical Engineering Journal

Volumn 41, Issue 1, 2008, Pages 67-73

  Ye, Ziqiang ^a   Song, Ge ^a   Chen, Guoqiang ^a,c   Chen, Jingyu ^b  

^a TSINGHUA UNIVERSITY   (China)

^b CHINA AGRICULTURAL UNIVERSITY   (China)

^c SHANTOU UNIVERSITY   (China)

Author keywords

Amino acids; Biosynthesis; DNA; PHA synthase; Polyhydroxyalkanoates; Recombinant DNA

Indexed keywords

BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; ESTERS; RHENIUM; RHENIUM ALLOYS;

C TERMINUS; CLASS I; MICROBIAL POLYESTERS; N TERMINALS; PHA SYNTHASES; POLYHYDROXYALKANOATE (PHA); SYNTHASE; SYNTHESIS (OF CHIRAL IONIC LIQUIDS);

ENZYMES;

3 HYDROXYBUTYRIC ACID; 3 HYDROXYHEXANOATE; HEXANOIC ACID; MONOMER; POLYESTER; POLYHYDROXYALKANOATE SYNTHASE; POLYHYDROXYALKANOIC ACID; RECOMBINANT DNA;

AEROMONAS HYDROPHILA; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; CUPRIAVIDUS NECATOR; ENZYME ACTIVITY; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; PSEUDOMONAS; SYNTHESIS;

AEROMONAS HYDROPHILA; CUPRIAVIDUS NECATOR; PSEUDOMONAS STUTZERI;

EID: 45449097050     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2008.03.006     Document Type: Article

References (37)

1. Anderson A.J., and Dawes E.A. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Mol. Biol. Rev. 54 (1990) 450-472
2. Jendrossek D., Schirmer A., and Schlegel H.G. Biodegradation of polyhydroxyalkanoic acids. Appl. Microbiol. Biotechnol. 46 (1996) 451-463
3. Williams S.F., Martin D.P., and Horowitz D.H. PHA applications: addressing the price performance issue I: tissue engineering. Int. J. Biol. Macromol. 25 (1999) 111-121
4. Zheng Z., Bei F.F., Tian H.L., and Chen G.Q. Effects of crystallization of polyhydroxyalkanoate blend on surface physicochemical properties and interactions with rabbit articular cartilage chondrocytes. Biomaterials 26 (2005) 3537-3548
5. Madison L.L., and Huisman G.W. Metabolic engineering of poly(3-hydroxyalkanoates): from DNA to plastic. Microbiol. Mol. Biol. Rev. 63 (1999) 21-32
6. Rehm B.H.A., and Steinbüchel A. Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis. Int. J. Biol. Macromol. 25 (1999) 3-19
7. Sudesh K., Abe H., and Doi Y. Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters. Prog. Polym. Sci. 25 (2000) 1503-1555
8. Park S.J., Ahn W.S., Green P.R., and Lee S.Y. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains. Biomacromolecules 2 (2001) 248-254
9. Rehm B.H.A. Polyester synthases: natural catalysts for plastics. Biochem. J. 376 (2003) 15-33
10. Schubert P., Steinbüchel A., and Schlegel H.G. Cloning of the Alcaligenes eutrophus genes for synthesis of poly-beta-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli. J. Bacteriol. 170 (1988) 5837-5847
11. Peoples O.P., and Sinskey A.J. Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J. Biol. Chem. 264 (1989) 15298-15303
12. Solaiman D.K.Y., Ashby R.D., and Foglia T.A. Rapid and specific identification of medium-chain-length polyhydroxyalkanoate synthase gene by polymerase chain reaction. Appl. Microbiol. Biotechnol. 53 (2000) 690-694
13. Jia Y., Yuan W., Wodzinska J., Park C., Sinskey A.J., and Stubbe J. Mechanistic studies on class I polyhydroxybutyrate (PHB) synthase from Ralstonia eutropha: Class I and III synthases share a similar catalytic mechanism. Biochemistry 40 (2001) 1011-1019
14. McCool G.J., and Cannon M.C. PhaC and PhaR are required for polyhydroxyalkanoic acid synthase activity in Bacillus megaterium. J. Bacteriol. 183 (2001) 4235-4243
15. Steinbüchel A., and Silke H. Biochemical and molecular basis of microbial synthesis of polyhydroxyalkanoates in microorganisms. Adv. Biochem. Eng/Biotechnol. 71 (2001) 81-123
16. Tsuge T., Saito Y., Kikkawa Y., Hiraishi T., and Doi Y. Biosynthesis and compositional regulation of poly [(3-hydroxybutyrate)-co-(3-hydroxyhexanoate)] in recombinant Ralstonia eutropha expressing mutated polyhydroxyalkanoate synthase genes. Macromol. Biosci. 4 (2004) 238-242
17. Zheng Z., Li M., Xue X.J., Tian H.L., Li Z., and Chen G.Q. Mutation on N-terminus of polyhydroxybutyrate synthase of Ralstonia eutropha enhanced PHB accumulation. Appl. Microbiol. Biotechnol. 72 (2006) 896-905
18. Nomura C.T., and Taguchi S. PHA synthase engineering towards super-biocatalysts for custom-made biopolymers. Appl. Microbiol. Biotechnol. 73 (2007) 969-979
19. Taguchi S., Maehara A., Takase K., Nakahara M., Nakamura H., and Doi Y. Analysis of mutational effects of a polyhydroxybutyrate (PHB) polymerase on bacterial PHB accumulation using an in vivo assay system. FEMS Microbiol. Lett. 198 (2001) 65-71
20. Hoppensack A., Rehm B.H.A., and Steinbüchel A. Analysis of 4-phosphopantetheinylation of polyhydroxybutyrate synthase from Ralstonia eutropha: generation of beta-alanine auxotrophic Tn5 mutants and cloning of the panD gene region. J. Bacteriol. 181 (1999) 1429-1435
21. Rehm B.H.A., Antonio R.V., Spiekermann P., Amara A.A., and Steinbüchel A. Molecular characterization of the poly(3-hydroxybutyrate) (PHB) synthase from Ralstonia eutropha: in vitro evolution, site-specific mutagenesis and development of a PHB synthase protein model. Biochim. Biophys. Acta-Pro. Struct. Mol. Enzymol. 1594 (2002) 178-190
22. Amara A.A., and Rehm B.H.A. Replacement of the catalytic nucleophile cysteine-296 by serine in class II polyhydroxyalkanoate synthase from Pseudomonas aeruginosa-mediated synthesis of a new polyester: identification of catalytic residues. Biochem. J. 374 (2003) 413-421
23. Lu X.Y., Zhang W.J., Wu Q., and Chen G.Q. Molecular cloning and functional analysis of two polyhydroxyalkanoate synthases from two strains of Aeromonas hydrophila spp. FEMS Microbiol. Lett. 243 (2005) 149-155
24. Chen J.Y., Liu T., Zheng Z., Chen J.C., and Chen G.Q. Polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas stutzeri 1317 had different substrate specificities. FEMS Microbiol. Lett. 234 (2004) 231-237
25. Chen J.Y., Song G., and Chen G.Q. A lower specificity PhaC2 synthase from Pseudomonas stutzeri catalyses the production of copolyesters consisting of short-chain-length and medium-chain-length 3-hydroxyalkanoates. Antonie van Leeuwenhoek 89 (2006) 157-167
26. Simon R., Priefer U., and Puhler A. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Nat. Biotech. 1 (1983) 784-791
27. Kovach M.E., Elzer P.H., Hill D.S., Robertson G.T., Farris M.A., Roop II R.M., and Peterson K.M. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166 (1995) 175-176
28. Ramsay B.A., Lomaliza K., Chavarie C., Bubé B., Bataille P., and Ramsay J.A.B. Production of poly-(β-hydroxybutyric-co-β-hydroxyvaleric) acids. Appl. Environ. Microbiol. 56 (1990) 2093-2098
29. Sambrook J. Molecular Cloning: A Laboratory Manual. third ed. (2001), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
30. Brandl H.R.A., Gross R.W.L., and Fuller R.C. Pseudomonas oleovorans as a source of poly({beta}-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl. Environ. Microbiol. 54 (1988) 1977-1982
31. Pal S., and Paul A.K. Physico-chemical characteristics of poly(3-hydroxybutyric acid) isolated from Azotobacter chroococcum MAL-201. Curr. Sci. 83 (2002) 1565-1568
32. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
33. Doi Y., Kitamura S., and Abe H. Microbial synthesis and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate). Macromolecules 28 (1995) 4822-4828
34. Kawaguchi Y., and Doi Y. Kinetics and mechanism of synthesis and degradation of poly(3-hydroxybutyrate) in Alcaligenes eutrophus. Macromolecules 25 (1992) 2324-2329
35. Segrest J.P., de Loof H., Dohlman J.G., Brouillette C.G., and Anantharamaiah G.M. Amphipathic helix motif: classes and properties. Proteins 8 (1990) 103-117
36. Chou P., and Fasman G. Prediction of the secondary structure of proteins from their amino acid sequence. Adv. Enzymol. Relat. Areas Mol. Biol. 47 (1978) 45-148
37. Niamsiri N., Delamarre S.C., Kim Y.R., and Batt C.A. Engineering of chimeric class II polyhydroxyalkanoate synthases. Appl. Environ. Microbiol. 70 (2004) 6789-6799