Purification, characterization, and overexpression of thermophilic pectate lyase of bacillus sp. rn1 isolated from a hot spring in Thailand

Bioscience, Biotechnology and Biochemistry

Volumn 73, Issue 2, 2009, Pages 268-273

  Sukhumsiirchart, Wasana ^a   Kawanishi, Sakiko ^b   Deesukon, Warin ^a   Chansiri, Kosum ^a   Kawasaki, Haruhiko ^b   Sakamoto, Tatsuji ^b  

^a SRINAKHARINWIROT UNIVERSITY   (Thailand)

^b Osaka Prefecture University   (Japan)

Author keywords

Bacillus spp; Overexpression; Pectate lyase; Thermophilic enzyme

Indexed keywords

BACILLUS SP; BACILLUS SPP; CATION EXCHANGES; CULTURE FILTRATES; ELECTROSPRAY-IONIZATION MASS SPECTROMETRIES; GALACTURONIC ACIDS; HIGH TEMPERATURES; HIGH-PERFORMANCE ANION EXCHANGE CHROMATOGRAPHIES; OPTIMUM PH; OVEREXPRESSION; PECTATE LYASE; POLYGALACTURONIC ACIDS; RECOMBINANT ENZYMES; THAILAND; THERMOPHILIC ENZYME;

AMINES; AMINO ACIDS; BACTERIOLOGY; CALCIUM; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; DEGRADATION; ELECTROSPRAY IONIZATION; GENE ENCODING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HOT SPRINGS; MASS SPECTROMETRY; ORGANIC ACIDS; POLYSACCHARIDES; PURIFICATION;

ENZYMES;

BACILLUS SP.;

PECTATE LYASE; POLYSACCHARIDE LYASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; CHEMISTRY; CLASSIFICATION; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; PHYLOGENY; TEMPERATURE; THAILAND; THERMAL SPRING;

AMINO ACID SEQUENCE; BACILLUS; BASE SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION; HOT SPRINGS; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYSACCHARIDE-LYASES; RECOMBINANT PROTEINS; TEMPERATURE; THAILAND;

EID: 65249146105     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.80287     Document Type: Article

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