Collagen ultrastructural changes during stromal wound healing in organ cultured bovine corneas

Experimental Eye Research

Volumn 88, Issue 5, 2009, Pages 953-959

  Kamma Lorger, Christina S ^a   Boote, Craig ^a   Hayes, Sally ^a   Albon, Julie ^a   Boulton, Michael E ^a   Meek, Keith M ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

collagen; cornea; organ culture; stromal flaps; stromal swelling; trephine wounds; wound healing; X ray scattering

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; COLLAGEN; FORMALDEHYDE; WAX;

ANIMAL TISSUE; ARTICLE; CELL COUNT; CELL CULTURE; CELL DIFFERENTIATION; CELL SWELLING; CELL ULTRASTRUCTURE; CONTROLLED STUDY; CORNEA; CORNEA INJURY; COW; EMBEDDING; EPITHELIUM CELL; FROZEN SECTION; IMMUNOHISTOCHEMISTRY; MYOFIBROBLAST; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RADIATION SCATTERING; SKULL SURGERY; STROMA; WOUND HEALING;

ANIMALS; CATTLE; CELL DIFFERENTIATION; COLLAGEN; CORNEAL STROMA; FIBROBLASTS; ORGAN CULTURE TECHNIQUES; WOUND HEALING; X-RAY DIFFRACTION;

EID: 64849106654     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.exer.2008.12.005     Document Type: Article

References (50)

1. Birk D.E., and Trelstad R.L. Extracellular compartments in matrix morphogenesis: collagen fibril, bundle, and lamellar formation by corneal fibroblasts. J. Cell. Biol. 99 (1984) 2024-2033
2. Birk D.E., Fitch J.M., and Linsenmayer T.F. Organization of collagen types I and V in the embryonic chicken cornea. Investig. Ophthalmol. Vis. Sci. 27 (1986) 1470-1477
3. Birk D.E., Fitch J.M., Babiarz J.P., Doane K.J., and Linsenmayer T.F. Collagen fibrillogenesis in vitro: interaction of types I and V collagen regulates fibril diameter. J. Cell Sci. 95 (1990) 649-657
4. Boote C., Dennis S., Newton R.H., Puri H., and Meek K.M. Collagen fibrils appear more closely packed in the pre-pupillary cornea: optical and biomechanical implications. Investig. Ophthalmol. Vis. Sci. 44 7 (2003) 2941-2948
5. Boote C., Dennis S., and Meek K. Spatial mapping of collagen fibril organisation in primate cornea. Investig. Ophthalmol. Vis. Sci. 45 (2004) E-Abstract 3821
6. Carrington L.M., and Boulton M. Hepatocyte growth factor and keratinocyte growth factor regulation of epithelial and stromal corneal wound healing. J. Cataract. Refract. Surg. 31 (2005) 412-423
7. Carrington L.M., Albon J., Anderson I., Kamma C., and Boulton M. Differential regulation of key stages in early corneal wound healing by TGF-ß isoforms and their inhibitors. Investig. Ophthalmol. Vis. Sci. 47 (2006) 1886-1894
8. Chakravarti S., Magnuson T., Lass J., Jepsen K., LaMantia C., and Carroll H. Lumican regulates collagen fibril assembly: skin fragility and corneal opacity in the absence of lumican. J. Cell Biol. 141 (1998) 1277-1286
9. Chakravarti S., Petroll W., Hassell J., Jester J., Lass J., Paul J., and Birk D. Corneal opacity in lumican-null mice: defects in collagen fibril structure and packing in the posterior stroma. Investig. Ophthalmol. Vis. Sci. 41 (2000) 3365-3373
10. Cintron C., and Hong B.S. Heterogeneity of collagens in rabbit cornea: type VI collagen. Investig. Ophthalmol. Vis. Sci. 29 (1988) 760-766
11. Cintron C., Hassinger L.C., Kublin C.L., and Cannon D.J. Biochemical and ultrastructural changes in collagen during corneal wound healing. J. Ultrastruct. Res. 65 (1978) 13-22
12. Connon C.J., and Meek K.M. The structure and swelling of corneal scar tissue in penetrating full-thickness wounds. Cornea 23 (2004) 165-171
13. Dawson D.G., Kramer T.R., Grossniklaus H.E., Waring G.O., and Edelhauser H.F. Histologic, ultrastructural, and immunofluorescent evaluation of human laser-assisted in situ keratomileusis corneal wounds. Arch. Ophthalmol. 123 (2005) 741-756
14. Edelhauser H. The resiliency of the corneal endothelium to refractive and intraocular surgery. Cornea 19 (2000) 263-273
15. Foreman D.M., Pancholi S., Jarvis-Evans J., McLeod D., and Boulton M.E. A simple organ culture model for assessing the effects of growth factors on corneal re-epithelization. Exp. Eye Res. 62 (1996) 555-564
16. Freund D.E., McCally R.L., and Farrell R.A. Effects of fibril orientations on light scattering in the cornea. J. Opt. Soc. Am. A 3 (1986) 1970-1982
17. Fullwood N.J., and Meek K.M. An ultrastructural, time-resolved study of freezing in the corneal stroma. J. Mol. Biol. 236 (1994) 749-758
18. Funderburgh J.L., Hevelone N.D., Roth M.R., Funderburgh M.L., Rodrigues M.R., Nirankari V.S., and Conrad G.W. Decorin and biglycan of normal and pathologic human corneas. Investig. Ophthalmol. Vis. Sci. 39 (1998) 1957-1964
19. Hassell J.R., Cintron C., Kublin C., and Newsome D.A. Proteoglycan changes during restoration of transparency in corneal scars. Arch. Biochem. Biophys. 222 (1983) 362-369
20. Helena M.C., Baerveldt F., Kim W.J., and Wilson S.E. Keratocyte apoptosis after corneal surgery. Investig. Ophthalmol. Vis. Sci. 39 (1998) 276-283
21. Hogan M.J., Alvarado J.A., and Weddell J.E. The Cornea: Histology of the Human Eye (1971), WB Saunders, Philadelphia
22. Ishizaki M., Wakamatsu K., Matsunami T., Yamanaka N., Saiga T., Shimizu Y., Zhu G., and Kao W.W. Dynamics of the expression of cytoskeleton components and adherens molecules by fibroblastic cells in alkali-burned and lacerated corneas. Exp. Eye Res. 59 (1994) 537-549
23. Ivarsen A., Laurberg T., and Møller-Pedersen T. Role of keratocyte loss on corneal wound repair after LASIK. Investig. Ophthalmol. Vis. Sci. 45 (2004) 3499-3506
24. Jester J.V., Petroll W.M., Barry P.A., and Cavanagh H.D. Expression of α-smooth muscle (α-SM) actin during corneal stromal wound healing. Investig. Ophthalmol. Vis. Sci. 36 (1995) 809-827
25. Jester J.V., Petroll W.M., and Cavanagh H.D. Corneal stromal wound healing in refractive surgery: the role of myofibroblasts. Prog. Retin Eye Res. 18 (1999) 311-356
26. Kim K.S., Jean S.J., and Edelhauser H.F. Corneal endothelial morphology and barrier function following excimer laser photorefractive keratectomy. In: Lass J.H. (Ed). Advances in Corneal Research. Selected Transactions of the World Congress on the Cornea IV (1997), Plenum Press, New York 329-342
27. Maguen E., Maguen B., Regev L., and Ljubimov A.V. Immunohistochemical evaluation of two corneal buttons with post-LASIK keratectasia. Cornea 26 (2007) 983-991
28. Maurice D.M. The structure and transparency of the cornea. J. Physiol. 136 2 (1957) 263-286
29. May C.A., Priglinger S.G., Neubauer A.S., Alge C.S., Ludwig K., Kampik A., and Welge-Lu U. Laser in situ keratomileusis in human corneas: new organ culture model. J. Cataract. Refract. Surg. 30 (2004) 179-186
30. McCally R.L., Freund D.E., Zorn A., Bonney-Ray J., Grebe R., de la Cruz Z., and Green W.R. Light-scattering and ultrastructure of healed penetrating corneal wounds. Investig. Ophthalmol. Vis. Sci. 48 (2007) 157-165
31. Meek K.M., and Leonard D.W. Ultrastructure of the corneal stroma: a comparative study. Biophys. J. 64 (1993) 273-280
32. Meek K.M., and Quantock A.J. The use of X-ray scattering techniques to determine corneal ultrastructure. Prog. Retin. Eye Res. 20 (2001) 95-137
33. Meek K.M., Fullwood N.J., Cooke P.H., Elliott G.F., Maurice D.M., Quantock A.J., Wall R.S., and Worthington C.R. Synchrotron X-ray diffraction studies of the cornea with implications for stromal hydration. Biophys. J. 60 (1991) 467-474
34. Mohan R.R., Hutcheon A.E., Choi R., Hong J., Lee J., Mohan R.R., Ambrosio Jr. R., Zieske J.D., and Wilson S.E. Apoptosis, necrosis, proliferation, and myofibroblast generation in the stroma following LASIK and PRK. Exp. Eye Res. 76 (2003) 71-78
35. Määttä M., Väisänen T., Väisänen M.R., Pihlajaniemi T., and Tervo T. Altered expression of type XIII collagen in keratoconus and scarred human cornea: increased expression in scarred cornea is associated with myofibroblast transformation. Cornea 25 (2006) 448-453
36. Netto M.V., Mohan R.R., Ambrósio Jr. R., Hutcheon A.E., Zieske J.D., and Wilson S.E. Wound healing in the cornea: a review of refractive surgery complications and new prospects for therapy. Cornea 24 (2005) 509-522
37. Priglinger S.G., May C.A., Alge C.S., Wolf A., Neubauer A.S., Haritoglou C., Kampik A., and Welge-Lussen U. Immunohistochemical findings after LASIK confirm in vitro LASIK model. Cornea 25 (2006) 331-335
38. Quantock A.J., Meek K.M., and Thonar E.J. Analysis of high angle synchrotron x-ray diffraction patterns obtained from macular dystrophy corneas. Cornea 11 (1992) 185-190
39. Richard N.R., Anderson J.A., Weiss J.L., and Binder P.S. Air/liquid corneal organ culture: a light microscopic study. Curr. Eye Res. 10 (1991) 739-749
40. Saika S. Alterations in extracellular matrix in experimental alkali bum of the cornea. In: Nishida T. (Ed). Santen International Symposium ProceedingsCorneal Healing Responses to Injuries and Refractive Surgeries (1998), Kugler Publications, The Hague 89-101
41. Schwarz W., and Keyserlingk D.G. Electron microscopy of normal and opaque human cornea. In: Langham M.E. (Ed). The Cornea. Macromolecular Organisation of a Connective Tissue (1967), Johns Hopkins Press, Baltimore/London 123-132
42. Sonigo B., Iordanidou V., Chong-Sit D., Auclin F., Ancel J.M., Labbé A., and Baudouin C. In vivo corneal confocal microscopy comparison of intralase femtosecond laser and mechanical microkeratome for laser in situ keratomileusis. Investig. Ophthalmol. Vis. Sci. 47 (2006) 2803-2811
43. Vainshtein E.S., and Urmakher L.S. A new method of x-ray determination of foreign body localization in the eye. Vestn. Oftal'mol. 79 (1966) 64-70
44. Vesaluoma M.H., Petroll W.M., Perez-Santonja J.J., Valle T.U., Alio J.L., and Tervo T.M. Laser in situ keratomileusis flap margin: wound healing and complications imaged by in vivo confocal microscopy. Am. J. Ophthalmol. 130 (2000) 564-573
45. Vij N., Roberts L., Joyce S., and Chakravarti S. Lumican suppresses cell proliferation and aids Fas-Fas ligand mediated apoptosis: implications in the cornea. Exp. Eye Res. 78 (2004) 957-971
46. Wilson S.E. Analysis of the keratocyte apoptosis, keratocyte proliferation, and myofibroblast transformation responses after photorefractive keratectomy and laser in situ keratomileusis. Trans. Am. Ophthalmol. Soc. 100 (2002) 411-433
47. Wilson S.E., and Kim W.J. Keratocyte apoptosis: implications on corneal wound healing, tissue organisation and disease. Investig. Ophthalmol. Vis. Sci. 39 (1998) 220-226
48. Wilson S.E., Mohan R.R., Hutcheon A.E., Mohan R.R., Ambrosio R., Zieske J.D., Hong J., and Lee J. Effect of ectopic epithelial tissue within the stroma on keratocyte apoptosis, mitosis and myofibroblast transformation. Exp. Eye Res. 76 (2003) 193-201
49. Worthington C.R., and Inouye H. X-ray diffraction study of the cornea. Int. J. Biol. Macromol. 7 (1985) 2-8
50. Zhao B., Cooper L.J., Brahma A., MacNeil S., Rimmer S., and Fullwood N.J. Development of a three-dimensional organ culture model for corneal wound healing and corneal transplantation. Investig. Ophthalmol. Vis. Sci. 47 (2006) 2840-2846