A high-yield and scaleable adenovirus vector production process based on high density perfusion culture of HEK 293 cells as suspended aggregates

Journal of Bioscience and Bioengineering

Volumn 107, Issue 5, 2009, Pages 524-529

  Liu, Hong ^a   Liu, Xing Mao ^a   Li, Shi Chong ^a   Wu, Ben Chuan ^a   Ye, Ling Ling ^a   Wang, Qi Wei ^a   Chen, Zhao Lie ^a  

^a BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

Author keywords

Aggregates; Gene therapy; HEK 293 cells; Perfusion culture; Production; Recombinant adenoviral vector

Indexed keywords

A DENSITIES; ADENOVIRUS VECTORS; CELL AGGREGATES; CELL DENSITIES; GENE THERAPY; HEK 293 CELLS; HEK-293; HIGH DENSITIES; HUMAN EMBRYONIC KIDNEY CELLS; INFECTION PROCESS; MEAN DIAMETERS; PERFUSION CULTURE; PRODUCTION KINETICS; PRODUCTION PROCESS; RECOMBINANT ADENOVIRAL VECTOR; RECOMBINANT ADENOVIRUS; SCALEABLE; SPHERICAL AGGREGATES; SPINNER FLASK CULTURES; SPINNER FLASKS; STIRRED TANK BIOREACTORS; STIRRED VESSELS; VIABLE CELLS; VIRUS CONCENTRATIONS; VOLUMETRIC PRODUCTIVITIES;

AGGREGATES; BIOREACTORS; BOTTLES; CELL IMMOBILIZATION; CELLS; GENETIC ENGINEERING; INDUSTRIAL CHEMICALS; TANKS (CONTAINERS); VECTORS; VIRUSES;

CELL CULTURE;

ADENOVIRUS VECTOR;

ARTICLE; BIOREACTOR; CELL AGGREGATION; CELL CULTURE; CELL DENSITY; CELL GROWTH; CELL STRAIN HEK293; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMOBILIZATION; VOLUMETRY;

ADENOVIRIDAE; CELL CULTURE TECHNIQUES; CELL LINE; GENETIC VECTORS; HUMANS; KIDNEY; PERFUSION; VIRUS CULTIVATION;

ADENOVIRIDAE;

EID: 64749084908     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2009.01.004     Document Type: Article

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