Suspended aggregates as an immobilization mode for high-density perfusion culture of HEK 293 cells in a stirred tank bioreactor

Applied Microbiology and Biotechnology

Volumn 72, Issue 6, 2006, Pages 1144-1151

  Liu, X M ^a   Liu, H ^a   Wu, B C ^a   Li, S C ^a   Ye, L L ^a   Wang, Q W ^a   Huang, P T ^a   Chen, Z L ^a  

^a BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

IMMOBILIZATION SYSTEMS; PERFUSION SYSTEMS; STIRRED TANK BIOREACTORS; STIRRED VESSELS;

BIOLOGICAL ORGANS; BIOREACTORS; CELL IMMOBILIZATION; DISSOCIATION; GROWTH KINETICS;

CELL CULTURE;

AGGREGATE SIZE; BIOREACTOR; BIOTECHNOLOGY; CELL ORGANELLE; IMMOBILIZATION;

ARTICLE; CD 293 MEDIUM; CELL AGGREGATION; CELL CULTURE; CELL DENSITY; CELL GROWTH; CELL POPULATION; CELL STRAIN HEK293; CELL SUSPENSION; CELL VIABILITY; CULTURE MEDIUM; DULBECCO MODIFIED EAGLE MEDIUM; EMBRYO; HAM E 12 MEDIUM; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; INOCULATION; LARGE SCALE PRODUCTION; MONOLAYER CULTURE; PERFUSION; PERFUSION CULTURE; SERUM; STIRRED REACTOR;

BIOREACTORS; CELL AGGREGATION; CELL COUNT; CELL CULTURE TECHNIQUES; CELL LINE; CELL SURVIVAL; CELLS, IMMOBILIZED; CULTURE MEDIA; HUMANS; MICROSCOPY; MICROSCOPY, ELECTRON, SCANNING;

EID: 33749828589     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0409-3     Document Type: Article

References (27)

1. Baldi L, Muller N, Picasso S, Jacquet R, Girard P, Thanh HP, Derow E, Wurm FM (2005) Transient gene expression in suspension HEK-293 cells: application to large-scale protein production. Biotechnol Prog 21(1):148-153
2. Butler M (2005) Animal cell cultures: recent achievement and perspectives in the production of biopharmaceuticals. Appl Microbiol Biotechnol 68:283-291
3. Chevalot I, Visvikis A, Nabet P, Engasser JM, Marc A (1994) Production of a membrane-bound protein, the human gamma-glutamyl transferase, by CHO cells cultivated on microcarriers, in aggregates and in suspension. Cytotechnology 16:121-129
4. Chu L, Robinson DK (2001) Industrial choices for protein production by large-scale cell culture. Curr Opin Biotechnol 12:180-187
5. Garham FL, Smiley J, Russel WC, Nairn R (1977) Characteristics of human cell line transformed by DNA from human adenovirus 5. J Gen Virol 36:59-72
6. Goetgheheur S, Hu WS (1991) Cultivation of anchorage-dependent animal cells in microsphere induced aggregate culture. Appl Microbiol Biotechnol 34:735-741
7. Grunberg J, Knogle K, Waibel R, Novak-Hofer I (2003) High-yield production of recombinant antibody fragments in HEK-293 cells using sodium butyrate. Biotechniques 34(5):968-972
8. Iyer P, Ostrove JM, Vacante D (1999) Comparison of manufacturing techniques for adenovirus production. Cytotechnology 30:169-172
9. Jones J, Nivitchanyong T, Giblin C, Ciccarone V, Judd D, Gorfien S, Krag SS, Betenbaugh MJ (2005) Optimization of tetracycline-responsive recombinant protein production and effect on cell growth and ER stress in mammalian cells. Biotechnol Bioeng 91(6):722-732
10. Kadouri A, Spier RE (1997) Some myths and messages concerning the batch and continuous culture of animal cells. Cytotechnology 24:89-98
11. Kretzmer G (2002) Industrial processes with animal cells. Appl Microbiol Biotechnol 59:135-142
12. Moreira JL, Alves PM (1995) Hydrodynamic effects on BHK cells grown as suspended natural aggregates. Biotechnol Bioeng 46:351-360
13. Moreira JL, Alves PM, Rodrigues JM, Cruz PE, Aunins JG, Carrondo MJT (1994) Studies of baby hamster kidney (BHK) natural cell aggregates in suspended batch cultures. Ann N Y Acad Sci 745:122-133
14. Nadeau I, Kemen A (2003) Production of adenovirus vector for gene therapy. Biotechnol Adv 20:475-489
15. Nikolai TJ, Hu WS (1992) Cultivation of mammalian cell on macroporous microcarriers. Enzyme Microb Technol 14:203-208
16. Peshwa MV, Kyung YS, McClure DB, Hu WS (1993) Cultivation of mammalian cells as aggregates in bioreactors: effect of calcium concentration on spatial distribution of viability. Biotechnol Bioeng 41:179-187
17. Reiter M, Hoenwarter O, Gaida T, Zach N, Schmatz C, Cluml G, Weigan F, Nilsson K, Katinger H (1990) The use of macroporous gelatin carriers for the cultivation of mammalian cells in fluidized bed reactors. Cytotechnology 3:271-277
18. Renner WA, Jordan M, Eppenberger HM, Leist C (1993) Cell-cell adhesion and aggregation: influence on the growth behavior of CHO cells. Biotechnol Bioeng 41:188-193
19. Ryu JH, Oh DJ, Choi CY, Kim BS (2003) Suspension culture of anchorage-dependent animal cells using nanospheres of the biodegradable polymer, poly (lactic-co-glycolic acid). Biotechnol Lett 25:1363-1367
20. Ryu JH, Kim MS, Lee GM, Choi CY, Kim BS (2005) The enhancement of recombinant protein production by polymer nanospheres in cell suspension culture. Biomaterials 26:2173-2181
21. Sinacore MS, Drapeau D, Adamson SR (2000) Adaptation of mammalian cells to growth in serum-free media. Mol Biotechnol 15:249-257
22. Tolbert WR, Hitt MM, Feder J (1980) Cell aggregate suspension culture for large-scale production of biomolecules. In Vitro 16:486-490
23. Voisard D, Meuwlt F, Ruffieux PA, Baer G, Kadouri A (2003) Potential of cell retention techniques for large scale highdensity perfusion culture of suspended mammalian cells. Biotechnol Bioeng 82(7):751-765
24. Wu N, Ataai MM (2000) Production of viral vectors for gene therapy applications. Curr Opin Biotechnol 11:205-208
25. Wurm FM (2004) Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 22:1393-1398
26. Yamada K, Kamihira M, Iijima S (1999) Enhanced cell aggregation and liver functions using polymers modified with a cell-specific ligand in primary hepatocyte culture. J Biotechnol Bioeng 88(5):557-562
27. Yamamoto S, Matsuda H, Yakahashi T, Xing XH, Tanji Y, Unno H (2000) Aggregate formation of rCHO cells and its maintenance in repeated batch culture in the absence of cell adhesion materials. J Biosci Bioeng 89:534-538