Detection of PAI-1 mRNA expression induced by TGF-β1 in keloid fibroblasts using SYBR real-time fluorescence quantitative RT-PCR method

Chinese Pharmacological Bulletin

Volumn 25, Issue 1, 2009, Pages 64-67

  He, Shu Fang ^a   Yang, Lin ^a   Huang, Wei Juan ^a   Yang, Yan ^a,b   Zhou, Fu Sheng ^a,b   Fang, Qiao Yun ^a,b   Zhang, An Ping ^a,b   Yang, Sen ^a,b   Zhang, Xue Jun ^a,b  

^a ANHUI MEDICAL UNIVERSITY   (China)

^b BEIJING UNIVERSITY OF POSTS AND TELECOMMUNICATIONS   (China)

Author keywords

Keloid; Plasminogen activator inhibitor 1; Real time fluorescence quantitative RT PCR; Transforming growth factor beta 1

Indexed keywords

BETA ACTIN; MESSENGER RNA; PLASMINOGEN ACTIVATOR INHIBITOR 1; TRANSFORMING GROWTH FACTOR BETA1;

ARTICLE; CELL ISOLATION; CELL STIMULATION; CONTROLLED STUDY; FIBROBLAST; FLUORESCENCE; GENE EXPRESSION REGULATION; IN VITRO STUDY; KELOID; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; UPREGULATION;

EID: 64549124394     PISSN: 10011978     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Yu H, Bock O, Bayat A, et al. Decreased expression of inhibitory SMAD6 and SMAD7 in keloid scarring[J]. J Plast Reconstr Aesthet Surg, 2006, 59(3):221-9.
2. Li H B, Cai J L, Liu Z Z, et al. Application evaluation of comprehensive standard for diagnosis and treatment of keloid [J]. Chin J Aesth Med, 2004, 13(3):361-2.
3. Pfaffl M W. A new mathematical model for relative quantification in real-time RT-PCR[J]. Nucleic Acids Res, 2001, 29(9):E45.
4. Wang X, Wang H M. Methodological classification of real-time quantitative PCR[J]. Chin J Clin Laborat Sci, 2007, 25(1):71-2.
5. Liu H, Wei W. TGF-β signal pathway and anti TGF-β strategies for treatment of liver fibrosis[J]. Chin Pharmacol Bull, 2007,23(5): 561-5.
6. Hasegawa T, Nakao A, Sumiyoshi K, et al. IFN-γ fails to antagonize fibrotic effect of TGF-β on keloid-derived dermal fibroblasts[J]. J Dermad Sci, 2003, 32(1):19-24.
7. 1/Smad signaling in myofibroblasts[J]. J Ethnopharmacol, 2008,118(2): 264-70.
8. Tuan T L, Wu H, Huang E Y, et al. Increased plasminogen activator inhibitor-1 in keloid fibroblasts may account for their elevated collagen accumulation in fibrin gel cultures[J]. Am J Pathol, 2003, 162(5):1579-89.
9. 1-induced PAI-1 gene expression requires MEK activity and cell-to-substrate adhesion[J]. J Cell Sci, 2001, 114(21):3905-14.
10. Wu Y, Zhang Q, Ann D K, et al. Increased vascular endothelial growth factor may account for elevated level of plasminogen activator inhibitor-1 via activating ERK1/2 in keloid fibroblasts[J]. Am J Physiol Cell Physiol, 2001,286(4):C905-12.
11. 1/ MAPK-mediated PAI-1 gene expression by the actin cytoskeleton in human mesangial cells[J]. Exp Cell Res, 2007,313(6):1240-50.
12. Wang L Y, Hong Q H, Zhang Y Z. Real-time quantitative PCR and its applications[J]. Chin J Cell Biol, 2004,26(1):62-6.
13. Zhao Y T, Zhang L F, Jin J. Establishment of a real-time fluorescence qantitative RT-PCR for measurement of t-PA mRNA expression in endothelial cells[J]. Chin Pharmacol Bull, 2006, 22 (5)633-7.