Establishment of a real-time fluorescence quantitative RT-PCR for measurement of t-PA mRNA expression in endothelial cells

Chinese Pharmacological Bulletin

Volumn 22, Issue 5, 2006, Pages 633-637

  Zhao, Yan Ting ^a   Zhang, Lian Fen ^a   Jin, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Human microvascular endothelial cells; Real time RT PCR; Tissue plasminogen activator

Indexed keywords

BETA ACTIN; MESSENGER RNA; RETINOIC ACID; TISSUE PLASMINOGEN ACTIVATOR;

ARTICLE; CELL LINE; CONTROLLED STUDY; CORRELATION ANALYSIS; ENDOTHELIUM CELL; FLUORESCENCE; HOUSEKEEPING GENE; HUMAN; HUMAN CELL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; UPREGULATION;

EID: 33747192063     PISSN: 10011978     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Huber D, Cramer E M, Kaufmann J E, et al. Tissue-type plasminogen activator (t-PA) is stored in Weibel-Palade bodies in human endothelial cells both in vitro and in vivo[J]. Blood, 2002, 99 (10):3637-45.
2. Wong M L, Medrano J F. Real-time PCR for mRNA quantitation[J]. Bio Techniques, 2005, 39(1):1-11.
3. Pattyn F, Speleman F, de Paepe A, Vandesompele J. RT Primer-DB: the Real-Time PCR primer and probe database[J]. Nucleic Acids Res, 2003, 31(1):122-3.
4. Baudry D, Faussillon M, Cabanis M O. Changes in WT1 splicing are associated with a specific gene expression profile in Wilms' tumour[J]. Oncogene, 2002, 21(36):5566-73.
5. Ades E W, Candal F J, Swerlick R A, et al. HMEC-1: establishment of an immortalized human microvascular endothelial cell line[J]. J Invest Dermatol, 1992, 99(6):683-90.
6. Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2[-Delta Delta C (T)] method[J]. Methods, 2001, 25(4):402-8.
7. Simpson D A, Feeney S, Boyle C, Stitt A W. Retinal VEGF mRNA measured by SYBR Green I fluorescence: A versatile approach to quantitative PCR[J]. Mol Vis, 2000, 6:178-83.
8. Ririe K M, Rasmussen R P, Wittwer C T. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction[J]. Anal Biochem, 1997, 245(2):154-60.
9. Pfaffl M W. A new mathematical model for relative quantification in real-time RT-PCR[J]. Nucleic Acids Res, 2001, 29(9):2002-7.