Activity of ethanol-stressed Oenococcus oeni cells: A flow cytometric approach

Journal of Applied Microbiology

Volumn 106, Issue 5, 2009, Pages 1690-1696

  Da Silveira, M G ^a   Abee, T ^b  

^a UNIVERSITY OF THE AZORES   (Portugal)

^b WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Adaptation; Ethanol; Flow cytometer; Malolactic fermentation; Oenococcus oeni; Stress

Indexed keywords

CELLS; CYTOLOGY; EXTRUSION; FERMENTATION; FLUORESCENCE; WINE;

ADAPTATION; CARBOXY FLUORESCEINS; CYTOMETERS; EXPOSED TO; FLOW CYTOMETER; FLUORESCENT PROBES; MALOLACTIC FERMENTATION; METABOLIC ACTIVITY; OENOCOCCUS OENI; SINGLE-CELL LEVEL;

ETHANOL;

ALCOHOL; FLUORESCEIN; FLUORESCENT DYE; MALIC ACID;

ADAPTATION; BACTERIUM; CELL ORGANELLE; ETHANOL; FERMENTATION; FLOW CYTOMETRY;

ARTICLE; BACTERIAL CELL; CELL ACTIVITY; CELL GROWTH; CELL METABOLISM; CELL SUBPOPULATION; EXPOSURE; FERMENTATION; FLOW CYTOMETRY; OENOCOCCUS OENI;

OENOCOCCUS OENI;

EID: 64149125819     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2008.04136.x     Document Type: Article

References (24)

1. Breeuwer, P., Drocourt, J.L., Rombouts, F.M. Abee, T. (1994) Energy dependent, carrier-mediated extrusion of carboxyfluorescein from Saccharomyces cerevisiae allows rapid assessment of cell viability by Flow cytometry. Appl Environ Microbiol 60, 1467 1472.
2. Breeuwer, P., Drocourt, J.L., Rombouts, F.M. Abee, T. (1996) A novel method for continuous determination of the intracellular pH in bacteria with the internally conjugated fluorescent probe of 5(and 6-)-carboxyfluorescein succinimidyl ester. Appl Environ Microbiol 62, 178 183.
3. Bunthof, C.J., Van Den Braak., S., Breeuwer, P., Rombouts, F.M. Abee, T. (1999) Rapid fluorescence assessment of the viability of stressed Lactococcus lactis. Appl Environ Microbiol 65, 3681 3689.
4. Cavin, J.F., Schmitt, P., Arias, A., Lin, J. Divies, C. (1988) Plasmid profiles in Leuconostoc species. Microbiol Aliment Nutr 6, 55 62.
5. Cox, D.J. Henick-Kling, T. (1989) The malolactic fermentation, a chemiosmotic energy-yielding (ATP) decarboxylation reaction. J Bacteriol 171, 5750 5752.
6. Da Silveira, M.G., San Romão, M.V., Loureiro-Dias, M.C., Rombouts, F.M. Abee, T. (2002) Flow cytometric assessment of membrane integrity of ethanol-stressed Oenococcus oeni cells. Appl Environ Microbiol 68, 6087 6093.
7. Da Silveira, M.G., Golovina, E.A., Hoekstra, F.A., Rombouts, F.M. Abee, T. (2003) Membrane fluidity adjustments in ethanol-stressed Oenococcus oeni cells. Appl Environ Microbiol 69, 5826 5832.
8. Davey, H. Kell, D. (1996) Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analysis. Microbiol Rev 60, 641 696.
9. + across the plasma membrane is not obligatory for bacterial growth. Science 197, 372 373.
10. Haugland, R.P. (1999) Handbook of Fluorescent Probes and Research Chemicals, 7th edn. New York, NY : Molecular Probes Inc.
11. Henick-Kling, T., Cox, D.J. Olsen, E.B. (1991) Prodution de l'énergie durant la fermentation malolactique. Rev Œnol 132, 63 66.
12. Lafon-Lafourcade, S., Carre, E. Ribéreau-Gayon, P. (1983) Occurrence of lactic acid bacteria during the different stages of vinification and conservation of wines. Appl Environ Microbiol 46, 874 880.
13. Lonvaud-Funel, A. (1999) Lactic acid bacteria in the quality improvement and depreciation of wine. Antonie van Leewenhoek 76, 317 331.
14. Loubiere, P., Salou, P., Leroy, M.J., Lindley, N.D. Pareilleux, A. (1992) Eletrogenic malate uptake and improved growth energetics of the malolactic bacterium Leuconostoc oenos grown on glucose-malate mixtures. J Bacteriol 174, 5302 5308.
15. Lowry, O.H., Rosebrough, N.J., Farr, A.L. Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193, 265 275.
16. Miranda, M., Ramos, A., Veiga-da-Cunha, M., Loureiro-Dias, M.C. Santos, H. (1997) Biochemical basis for glucose-induced inhibition of malolactic fermentation in Leuconostoc oenos. J Bacteriol 179, 5347 5354.
17. Molenaar, D., Bolhuis, H., Abee, T., Poolman, B. Konings, W.N. (1992) The efflux of a fluorescent probe is catalysed by an ATP-driven extrusion system in Lactococcus lactis. J Bacteriol 174, 3118 3124.
18. Olsen, E.B., Russell, J.B. Henick-kling, T. (1991) Electrogenic L-malate transport by Lactobacillus plantarum: a basis for energy derivation from malolactic fermentation. J Bacteriol 173, 6199 6206.
19. Poolman, B., Molenaar, D., Smid, E.J., Ubbink, T., Abee, T., Renault, P.P. Konings, W.N. (1991) Malolactic fermentation: electrogenic malate uptake and malate/lactate antiport generate metabolic energy. J Bacteriol 173, 6030 6037.
20. Salema, M., Poolman, B., Lolkema, J.S., Loureiro Dias, M.C. Konings, W.N. (1994) Uniport of monoanionic L-malate in membrane vesicles from Leuconostoc oenos. Eur J Biochem 225, 289 295.
21. Salema, M., Lolkema, J.S., San Romão, M.V. Loureiro Dias, M.C. (1996) The proton motive force generated in Leuconostoc oenos by L-malate fermentation. J Bacteriol 178, 3127 3132.
22. Salou, P., Loubiere, P. Pareilleux, A. (1994) Growth and energetics of Leuconostoc oenos during cometabolism of glucose with citrate or fructose. Appl Environ Microbiol 60, 1459 1466.
23. 13C magnetic resonance to elucidate the unexpected biosynthesis of erythritol by Leuconostoc oenos. Appl Environ Microbiol 58, 2271 2279.
24. Veiga-da-Cunha, M., Santos, H. van Schaftingen, E. (1993) Pathway and regulation of erythritol formation in Leuconostoc oenos. J Bacteriol 175, 3941 3948.