Differential expression of the interleukin 5 receptor α isoforms in blood and tissue eosinophils of nasal polyp patients

Allergy: European Journal of Allergy and Clinical Immunology

Volumn 64, Issue 5, 2009, Pages 725-732

  Gevaert, P ^a,b   Hellman, C ^c   Lundblad, L ^d   Lundahl, J ^c   Holtappels, G ^b   Van Cauwenberge, P ^b   Tavernier, J ^b   Bachert, C ^b  

^a GHENT UNIVERSITY HOSPITAL   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

^c Department of Clinical Immunology and Allergy ^*

^d KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Eosinophils; IL 5 receptor alpha; Nasal polyps; Rhinosinusitis

Indexed keywords

BECLOMETASONE DIPROPIONATE; CD69 ANTIGEN; CORTICOSTEROID; GLUCOCORTICOID; INTERLEUKIN 5; INTERLEUKIN 5 RECEPTOR ALPHA; MATRIX METALLOPROTEINASE 14;

ADULT; AGED; ARTICLE; ASTHMA; CLINICAL ARTICLE; CONTROLLED STUDY; DISEASE SEVERITY; DOWN REGULATION; ENZYME LINKED IMMUNOSORBENT ASSAY; EOSINOPHIL COUNT; FEMALE; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO SELECTION; IN VIVO STUDY; MALE; NOSE POLYP; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SECRETION; UPREGULATION;

ADOLESCENT; ADULT; AGED; ANTIGENS, CD; ANTIGENS, DIFFERENTIATION, T-LYMPHOCYTE; ASTHMA; EOSINOPHILS; FEMALE; GENE EXPRESSION; HUMANS; INTERLEUKIN-5; INTERLEUKIN-5 RECEPTOR ALPHA SUBUNIT; MALE; MIDDLE AGED; NASAL POLYPS; PROTEIN ISOFORMS; YOUNG ADULT;

EID: 63849118093     PISSN: 01054538     EISSN: 13989995     Source Type: Journal    
DOI: 10.1111/j.1398-9995.2008.01885.x     Document Type: Article

References (24)

1. Denburg JA, Sehmi R, Upham J. Regulation of IL-5 receptor on eosinophil progenitors in allergic inflammation: role of retinoic acid. Int Arch Allergy Immunol 2001 124 : 246 248.
2. Sanderson CJ. Interleukin-5, eosinophils, and disease. Blood 1992 79 : 3101 3109.
3. Cameron L, Christodoulopoulos P, Lavigne F, Nakamura Y, Eidelman D, McEuen A et al. Evidence for local eosinophil differentiation within allergic nasal mucosa: inhibition with soluble IL-5 receptor. J Immunol 2000 164 : 1538 1545.
4. Simon HU, Yousefi S, Schranz C, Schapowal A, Bachert C, Blaser K. Direct demonstration of delayed eosinophil apoptosis as a mechanism causing tissue eosinophilia. J Immunol 1997 158 : 3902 3908.
5. Leckie MJ, ten Brinke A, Khan J, Diamant Z, O'Connor BJ, Walls CM et al. Effects of an interleukin-5 blocking monoclonal antibody on eosinophils, airway hyper-responsiveness, and the late asthmatic response. Lancet 2000 356 : 2144 2148.
6. Gevaert P, Lang-Loidolt D, Lackner A, Stammberger H, Staudinger H, Van ZT et al. Nasal IL-5 levels determine the response to anti-IL-5 treatment in patients with nasal polyps. J Allergy Clin Immunol 2006 118 : 1133 1141.
7. Flood-Page PT, Menzies-Gow AN, Kay AB, Robinson DS. Eosinophil's role remains uncertain as anti-interleukin-5 only partially depletes numbers in asthmatic airway. Am J Respir Crit Care Med 2003 167 : 199 204.
8. Kay AB, Menzies-Gow A. Eosinophils and interleukin-5: the debate continues. Am J Respir Crit Care Med 2003 167 : 1586 1587.
9. Tavernier J, Devos R, Cornelis S, Tuypens T, Van der Heyden J, Fiers W et al. A human high affinity interleukin-5 receptor (IL5R) is composed of an IL5-specific alpha chain and a beta chain shared with the receptor for GM-CSF. Cell 1991 66 : 1175 1184.
10. Martinez-Moczygemba M, Huston DP, Lei JT. JAK kinases control IL-5 receptor ubiquitination, degradation, and internalization. J Leukoc Biol 2007 81 : 1137 1148.
11. Tavernier J, Van der Heyden J, Verhee A, Brusselle G, Van Ostade X, Vandekerckhove J et al. Interleukin 5 regulates the isoform expression of its own receptor alpha-subunit. Blood 2000 95 : 1600 1607.
12. Wang P, Wu P, Cheewatrakoolpong B, Myers JG, Egan RW, Billah MM. Selective inhibition of IL-5 receptor alpha-chain gene transcription by IL-5, IL-3, and granulocyte-macrophage colony-stimulating factor in human blood eosinophils. J Immunol 1998 160 : 4427 4432.
13. Liu LY, Sedgwick JB, Bates ME, Vrtis RF, Gern JE, Kita H et al. Decreased expression of membrane IL-5 receptor alpha on human eosinophils: II. IL-5 down-modulates its receptor via a proteinase- mediated process. J Immunol 2002 169 : 6459 6466.
14. Liu LY, Sedgwick JB, Bates ME, Vrtis RF, Gern JE, Kita H et al. Decreased expression of membrane IL-5 receptor alpha on human eosinophils: I. Loss of membrane IL-5 Receptor alpha on airway eosinophils and increased soluble IL-5 receptor alpha in the airway after allergen challenge. J Immunol 2002 169 : 6452 6458.
15. Gregory B, Kirchem A, Phipps S, Gevaert P, Pridgeon C, Rankin SM et al. Differential regulation of human eosinophil IL-3, IL-5, and GM-CSF receptor alpha-chain expression by cytokines: IL-3, IL-5, and GM-CSF down-regulate IL-5 receptor alpha expression with loss of IL-5 responsiveness, but up-regulate IL-3 receptor alpha expression. J Immunol 2003 170 : 5359 5366.
16. Gevaert P, Bachert C, Holtappels G, Novo CP, Van der Heyden J, Fransen L et al. Enhanced soluble interleukin-5 receptor alpha expression in nasal polyposis. Allergy 2003 58 : 371 379.
17. Bachert C, Gevaert P, Holtappels G, Cuvelier C, van Cauwenberge P. Nasal polyposis: from cytokines to growth. Am J Rhinol 2000 14 : 279 290.
18. Hansel TT, De Vries IJ, Iff T, Rihs S, Wandzilak M, Betz S et al. An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils. J Immunol Methods 1991 145 : 105 110.
19. Terstappen LW, de Grooth BG, Visscher K, van Kouterik FA, Greve J. Four-parameter white blood cell differential counting based on light scattering measurements. Cytometry 1988 9 : 39 43.
20. Perez C, Vandesompele J, Vandenbroucke I, Holtappels G, Speleman F, Gevaert P et al. Quantitative Real Time Polymerase Chain Reaction for measurement of human Interleukin-5 receptor alpha spliced isoforms mRNA. BMC Biotechnol 2003 3 : 17.
21. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002 3 : 34.
22. Julius P, Hochheim D, Boser K, Schmidt S, Myrtek D, Bachert C et al. Interleukin-5 receptors on human lung eosinophils after segmental allergen challenge. Clin Exp Allergy 2004 34 : 1064 1070.
23. Bachert C, Gevaert P, Holtappels G, Johansson SG, van Cauwenberge P. Total and specific IgE in nasal polyps is related to local eosinophilic inflammation. J Allergy Clin Immunol 2001 107 : 607 614.
24. Hellman C, Halldén G, Hylander B, Lundahl J. Regulation of the interleukin-5 receptor alpha-subunit on peripheral blood eosinophils from healthy subjects. Clin Exp Immunol 2003 131 : 75 81.