Synthesis, characterization, and bioconjugation of fluorescent gold nanoclusters toward biological labeling applications

ACS Nano

Volumn 3, Issue 2, 2009, Pages 395-401

  Lin, Cheng An J ^a   Yang, Ting Ya ^a   Lee, Chih Hsien ^a   Huang, Sherry H ^a   Sperling, Ralph A ^b   Zanella, Marco ^b   Li, Jimmy K ^a,d   Shen, Ji Lin ^a   Wang, Hsueh Hsiao ^c   Yeh, Hung I ^c   Parak, Wolfgang J ^b   Chang, Walter H ^a  

^a CHUNG YUAN CHRISTIAN UNIVERSITY   (Taiwan)

^b PHILIPPS UNIVERSITY MARBURG   (Germany)

^c MACKAY MEMORIAL HOSPITAL   (Taiwan)

^d HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Colloidal gold; Fluorescent nanoparticles; Nanoclusters

Indexed keywords

BIO CONJUGATIONS; BIOLOGICAL LABELING; BIOLOGICAL MOLECULES; COLLOIDAL GOLD; COLLOIDAL STABILITIES; FLUORESCENT NANOPARTICLES; GOLD NANOCLUSTERS; LIVING CELLS; ULTRA SMALLS;

FLUORESCENCE; GOLD; NANOPARTICLES;

NANOCLUSTERS;

BOVINE SERUM ALBUMIN; FLUORESCENT DYE; GOLD; MACROGOL DERIVATIVE; METAL NANOPARTICLE;

ANIMAL; ARTICLE; CATTLE; CELL SURVIVAL; CHEMISTRY; CYTOLOGY; ENDOTHELIUM CELL; ENZYME SPECIFICITY; GEL CHROMATOGRAPHY; HUMAN; METABOLISM; METHODOLOGY; STAINING; SYNTHESIS; TUMOR CELL LINE; ULTRAVIOLET SPECTROPHOTOMETRY;

ANIMALS; CATTLE; CELL LINE, TUMOR; CELL SURVIVAL; CHROMATOGRAPHY, GEL; ENDOTHELIAL CELLS; FLUORESCENT DYES; GOLD; HUMANS; METAL NANOPARTICLES; POLYETHYLENE GLYCOLS; SERUM ALBUMIN, BOVINE; SPECTROPHOTOMETRY, ULTRAVIOLET; STAINING AND LABELING; SUBSTRATE SPECIFICITY;

EID: 63449111279     PISSN: 19360851     EISSN: 1936086X     Source Type: Journal    
DOI: 10.1021/nn800632j     Document Type: Article

References (60)

1. Bigioni, T. P.; Whetten, R. L.; Dag, O. Near-Infrared Luminescence from Small Gold Nanocrystals. J. Phys. Chem. B 2000, 104, 6983-6986.
2. Huang, T.; Murray, R. W. Visible Luminescence of Water-Soluble Monolayer- Protected Gold Clusters, J. Phys. Chem, B 2001, 105, 12498-12502.
3. Peyser, L. A.; Vinson, A. E.; Bartko, A. P.; Dickson, R. M. Photoactivated Fluorescence from Individual Silver Nanoclusters. Science 2001, 291, 103-106.
4. Zheng, J.; Dickson, R. M. Individual Water-Soluble Dendrimer-Encapsulated Silver Nanodot Fluorescence. J. Am. Chem. Soc. 2002, 124, 13982-13983.
5. Link, S.; Beeby, A.; FitzGerald, S.; El-Sayed, M. A.; Schaaff, T. G.; Whetten, R. L. Visible to Infrared Luminescence from a 28-Atom Gold Cluster. J. Phvs. Chem. B 2002, 106, 3410-3415.
6. Yang, Y.; Chen, S. Surface Manipulation of the Electronic Energy of Subnanometer-Sized Gold Clusters: An Electrochemical and Spectroscopic Investigation. Nana Leff. 2003, 3, 75-79.
7. Zheng, J.; Petty, J. T.; Dickson, R. M. High Quantum Yield Blue Emission from Water-Soluble Au-8 Nanodots. J. Am. Chem. Soc. 2003, 125, 7780-7781.
8. Negishi, Y.; Takasugi, Y.; Sato, S.; Yao, H.; Kimura, K.; Tsukuda, T. Magic-Numbered Au-N Clusters Protected by Glutathione Monolayers (N = 18,21, 25, 28,32, 39): Isolation and Spectroscopic Characterization. J. Am. Chem. Soc. 2004, 126, 6518-6519.
9. Schaaff, T. G.; Whetten, R. L. Giant Gold-Glutathione Cluster Compounds: Intense Optical Activity in Metal-Based Transitions. J. Phvs. Chem. B 2000, 104, 2630-2641.
10. Garzon, I. L.; Reyes-Nava, J. A.; Rodriguez-Hernandez, J. I.; Sigal, I.; Beltran, M. R.; Michaelian, K. Chirality in Bare and Passivated Gold Nanoclusters. Phys. Rev. B 2002, 66 , 073403-1-073403-4.
11. Gautier, C.; Bürgi, T. Chiral Inversion of Gold Nanoparticles. J. Am. Chem. Soc. 2008, 130, 7077-7084.
12. Roman-Velazquez, C. E.; Noguez, C.; Garzon, I. L. Circular Dichroism Simulated Spectra of Chiral Gold Nanoclusters: A Dipole Approximation. J. Phys. Chem. B 2003, 107, 12035-12038.
13. Crespo, P.; Litran, R.; Rojas, T. C.; Multigner, M.; de la Fuente, J. M.; Sanchez-Lopez, J. C.; Garcia, M. A.; Hernando, A.; Penades, S.; Fernandez, A. Permanent Magnetism, Magnetic Anisotropy, and Hysteresis of Thiol-Capped Gold Nanoparticles. Phys. Rev. Lett. 2004, 93, 087204-1-087204-4.
14. Chen, S.; Ingram, R. S.; Hostetler, M. J.; Pietron, J. J.; Murray, R. W.; Schaaff, T. G.; Khoury, J. T.; Alvarez, M. M.; Whetten, R. L. Gold Nanoelectrodes of Varied Size: Transition to Molecule-like Charging. Science 1998, 280, 2098-2101.
15. Hicks, J. F.; Templeton, A. C.; Chen, S. W.; Sheran, K. M.; Jasti, R.; Murray, R. W.; Debord, J.; Schaaf, T. G.; Whetten, R. L. The Monolayer Thickness Dependence of Quantized Double-Layer Capacitances of Monolayer-Protected Gold Clusters. Anal. Chem. 1999, 71, 3703-3711.
16. Hicks, J. F.; Miles, D. T.; Murray, R. W. Quantized Double-Layer Charging of Highly Monodisperse Metal Nanoparticles.J. Am. Chem. Soc. 2002, 124, 13322-13328.
17. Quinn, B. M.; Liljeroth, P.; Ruiz, V.; Laaksonen, T.; Kontturi, K. Electrochemical Resolution of 15 Oxidation States for Monolayer Protected Gold Nanoparticles. J. Am. Chem. Soc. 2003, 125, 6644-6645.
18. Lee, T. H.; Gonzalez, J. I.; Zheng, J.; Dickson, R. M. Single-Molecule Optoelectronics. Acc. Chem. Res. 2005, 38, 534-541.
19. Vosch, T.; Antoku, Y.; Hsiang, J. C.; Richards, C. I.; Gonzalez, J. I.; Dickson, R. M. Strongly Emissive Individual DNA-Encapsulated Ag Nanoclusters as Single-Molecule Fluorophores. Proc. Natl. Acad. Sci. U.S.A. 2007, 104, 12616-12621.
20. Huang, C. C.; Yang, Z.; Lee, K. H.; Chang, H. T. Synthesis of Highly Fluorescent Gold Nanoparticles for Sensing Mercury(ll). Angew. Chem., Int. Ed. 2007, 46, 6824-6828.
21. Triulzi, R. C.; Micic, M.; Giordani, S.; Serry, M.; Chiou, W. A.; Leblanc, R. M. Immunoasssay Based on the Antibody-Conjugated Pamam-Dendrimer-Gold Quantum Dot Complex. Chem. Commun. 2006, 5068-5070.
22. Huang, C. C.; Chiang, C. K.; Lin, Z. H.; Lee, K. H.; Chang, H. T. Bioconjugated Gold Nanodots and Nanoparticles for Protein Assays Based on Photoluminescence Quenching. Anal. Chem. 2008, 80, 1497-1504.
23. Zheng, J.; Zhang, C. W.; Dickson, R. M. Highly Fluorescent, Water-Soluble, Size-Tunable Gold Quantum Dots. Phys. Rev. Lett. 2004, 93, 077402-1 -077402-4.
24. Link, S.; El-Sayed, M. A. Optical Properties and Ultrafast Dynamics of Metallic Nanocrystals. Annu. Rev. Phys. Chem. 2003, 54, 331-66.
25. Alvarez, M. M.; Khoury, J. T.; Schaaff, T. G.; Shafigullin, M. N.; Vezmar, I.; Whetten, R. L. Optical Absorption Spectra of Nanocrystal Gold Molecules. J. Phys. Chem. B 1997, 101, 3706-3712.
26. Schaaff, T. G.; Shafigullin, M. N.; Khoury, J. T.; Vezmar, I.; Whetten, R. L.; Cullen, W. G.; First, P. N.; GutierrezWing, C.; Ascensio, J.; JoseYacaman, M.J. Isolation of Smaller Nanocrystal Au Molecules: Robust Quantum Effects in Optical Spectra. J. Phys. Chem. B 1997, 101, 7885-7891.
27. Hostetler, M. J.; Wingate, J. E.; Zhong, C. J.; Harris, J. E.; Vachet, R. W.; Clark, M. R.; Londono, J. D.; Green, S. J.; Stokes, J. J.; Wignall, G. D.; et al Alkanethiolate Gold Cluster Molecules with Core Diameters from 1.5 to 5.2 Nm: Core and Monolayer Properties as a Function of Core Size. Langmuir 1998, 14, 17-30.
28. Schaeffer, N.; Tan, B.; Dickinson, C.; Rosseinsky, M. J.; Laromaine, A.; McComb, D. W.; Stevens, M. M.; Wang, Y.; Petit, L.; Barentin, C.; et al Fluorescent or Not? Size-Dependent Fluorescence Switching for Polymer-Stabilized Gold Clusters in the 1.1 -1.7 nm Size Range. Chem. Comm. 2008, 3986-3988.
29. Apell, P.; Monreal, R.; Lundqvist, S. Photoluminescence of Noble Metals. Phys. Scr. 1988, 38, 174-179.
30. Lee, D.; Donkers, R. L.; Wang, G. L.; Harper, A. S.; Murray, R. W. Electrochemistry and Optical Absorbance and Luminescence of Molecule-like Au-38 Nanoparticles. J. Am. Chem. Soc. 2004, 126, 6193-6199.
31. Lin, Z. Y.; Kanters, R. P. F.; Mingos, D. M. P. Closed-Shell Electronic Requirements for Condensed Clusters of the Group-11 Elements. Inorg. Chem. 1991, 30, 91-95.
32. Fedrigo, S.; Harbich, W.; Buttet, J. Optical-Response of Ag2, Ag3, Au2, and Au3 in Argon Matrices. J. Chem. Phys. 1993, 99, 5712-5717.
33. Konig, L.; Rabin, I.; Schulze, W.; Ertl, G. Chemiluminescence in the Agglomeration of Metal Clusters. Science 1996, 274, 1353-1355.
34. Wilcoxon, J. P.; Martin, J. E.; Parsapour, F.; Wiedenman, B.; Kelley, D. F. Photoluminescence from Nanosize Gold Clusters. J. Chem. Phys. 1998, 108, 9137-9143.
35. Mooradian, A. Photoluminescence of Metals. Phys. Rev. Lett. 1969, 22, 185-187.
36. Boyd, G. T.; Yu, Z. H.; Shen, Y. R. Photoinduced Luminescence from the Noble-Metals and Its Enhancement on Roughened Surfaces. Phys. Rev. B 1986, 33, 7923-7936.
37. Sperling, R. A.; Gil, P. R.; Zhang, F.; Zanella, M.; Parak, W. J. Biological Applications of Gold Nanoparticles. Chem. Soc. Rev. 2008, 37, 1896-1908.
38. Alivisatos, A. P. Semiconductor Clusters, Nanocrystals, and Quantum Dots. Science 1996, 271, 933-937.
39. Kirchner, C.; Liedl, T.; Kudera, S.; Pellegrino, T.; Javier, A. M.; Gaub, H. E.; Stolzle, S.; Fertig, N.; Parak, W. J. Cytotoxicity of Colloidal Cdse and Cdse/Zns Nanoparticles. Nano Lett. 2005, 5, 331-338.
40. Pan, Y.; Neuss, S.; Leifert, A.; Fischler, M.; Wen, F.; Simon, U.; Schmid, G.; Brandau, W.; Jahnen-Dechent, W. Size-Dependent Cytotoxicity of Gold Nanoparticles. Small 2007, 3, 1941-1949.
41. Negishi, Y.; Tsukuda, T. Visible Photoluminescence from Nearly Monodispersed Au-12 Clusters Protected by Meso-2,3-Dimercaptosuccinic Acid. Chem. Phys. Lett. 2004,383, 161-165.
42. Negishi, Y.; Nobusada, K.; Tsukuda, T. Glutathione-Protected Gold Clusters Revisited: Bridging the Gap between Gold(l)-Thiolate Complexes and Thiolate-Protected Gold Nanocrystals. J. Am, Chem. Soc. 2005, 127, 5261-5270.
43. Wang, G. L.; Huang, T.; Murray, R. W.; Menard, L.; Nuzzo, R. G. Near-lr Luminescence of Monolayer-Protected Metal Clusters. J. Am, Chem. Soc. 2005, 127, 812-813.
44. Cha, S. H.; Kim, J. U.; Kim, K. H.; Lee, J. C. Preparation and Photoluminescent Properties of Gold(l)-Alkanethiolate Complexes Having Highly Ordered Supramolecular Structures. Chem. Mater. 2007, 19, 6297-6303.
45. Scolari, M.; Vogel, E. K.; Awh, E. Perceptual Expertise Enhances the Resolution but Not the Number of Representations in Working Memory. Psychonom. Bull. Rev. 2008, 15, 215-222.
46. Sperling, R. A.; Liedl, T.; Duhr, S.; Kudera, S.; Zanella, M.; Lin, C.-A. J.; Chang, W. H.; Braun, D.; Parak, W. J. Size Determination of (Bio-)conjugated Water-Soluble Colloidal Nanoparticles - A Comparison of Different Techniques. J. Phys. Chem. C 2007, 111, 11552-11559.
47. Ballou, B.; Lagerholm, B. C.; Ernst, L A.; Bruchez, M. P.; Waggoner, A. S. Noninvasive Imaging of Quantum Dots in Mice. Bioconjugate Chem. 2004, 15, 79-86.
48. Bentzen, E. L.; Tomlinson, I. D.; Mason, J.; Gresch, P.; Warnement, M. R.; Wright, D.; Sanders-Bush, E.; Blakely, R.; Rosenthal, S. J. Surface Modification to Reduce Nonspecific Binding of Quantum Dots in Live Cell Assays. Bioconjugate Chem. 2005, 16, 1488-1494.
49. Dubertret, B.; Skourides, P.; Norris, D. J.; Noireaux, V.; Brivanlou, A. H.; Libchaber, A. In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles. Science 2002, 298, 1759-1762.
50. Parak, W. J.; Gerion, D.; Zanchet, D.; Woerz, A. S.; Pellegrino, T.; Micheel, C.; Williams, S. C.; Seitz, M.; Bruehl, R. E.; Bryant, Z.; et al. Conjugation of DNA to Silanized Colloidal Semiconductor Nanocrystaline Quantum Dots. Chem. Mater. 2002, 14, 2113-2119.
51. Nikolic, M. S.; Krack, M.; Aleksandrovic, V.; Kornowski, A.; Forster, S.; Weller, H. Tailor-Made Ligands for Biocompatible Nanoparticles. Angew. Chem., Int. Ed. 2006, 45, 6577-6580.
52. Mei, B. C.; Susumu, K.; Medintz, I. L.; Delehanty, J. B.; Mountziaris, T. J.; Mattoussi, H. Modular Poly(Ethylene Glycol) Ligands for Biocompatible Semiconductor and Gold Nanocrystals with Extended Ph and Ionic Stability. J. Mater. Chem. 2008, 18, 4949-4958.
53. Sperling, R. A.; Pellegrino, T.; Li, J. K.; Chang, W. H.; Parak, W. J. Electrophoretic Separation of Nanoparticles with a Discrete Number of Functional Groups. Adv. Funct. Mater. 2006, 16, 943-948.
54. Lin, C. A. J.; Sperling, R. A.; Li, J. K.; Yang, T. Y.; Li, P. Y.; Zanella, M.; Chang, W. H.; Parak, W. G. J. Design of an Amphiphilic Polymer for Nanoparticle Coating and Functionalization. Small 2008, 4, 334-341.
55. Wang, H.; Pevsner, J. Detection of Endogenous Biotin in Various Tissues: Novel Functions in the Hippocampus and Implications for Its Use in Avidin-Biotin Technology. Cell Tissue Res. 1999, 296, 511-516.
56. Parak, W.; Boudreau, R.; Le Gros, M.; Gerion, D.; Zanchet, D.; Micheel, C.; Williams, S.; Alivisatos, A.; Larabell, C. Cell Motility and Metastatic Potential Studies Based on Quantum Dot Imaging of Phagokinetic Tracks. Adv. Mater. 2002, 74, 882-885.
57. Jadzinsky, P. D.; Calero, G.; Ackerson, C. J.; Bushnell, D. A.; Kornberg, R. D. Structure of a Thiol Monolayer-Protected Gold Nanoparticle at 1.1 Angstrom Resolution. Science 2007, 318, 430-433.
58. Jana, N. R.; Peng, X. G. Single-Phase and Gram-Scale Routes toward Nearly Monodisperse Au and Other Noble Metal Nanocrystals. J. Am. Chem. Soc. 2003, 125, 14280-14281.
59. Kruger, D.; Fuchs, H.; Rousseau, R.; Marx, D.; Parrinello, M. Interaction of Short-Chain Alkane Thiols and Thiolates with Small Gold Clusters: Adsorption Structures and Energetics. J. Chem. Phys. 2001, 115, 4776-4786.
60. Mattoussi, H.; Mauro, J.; Goldman, E.; Anderson, G.; Sundar, V.; Mikulec, F.; Bawendi, M. Self-Assembly of Cdse-Zns Quantum Dot Bioconjugates Using an Engineered Recombinant Protein. J. Am. Chem. Soc. 2000, 122, 12142-12150.