PCR-induced sequence alterations hamper the typing of prehistoric bone samples for diagnostic achondroplasia mutations

Molecular Biology and Evolution

Volumn 21, Issue 11, 2004, Pages 2005-2011

  Pusch, C M ^a   Broghammer, M ^a   Nicholson, G J ^a   Nerlich, A G ^b   Zink, A ^b   Kennerknecht, I ^c   Bachmann, L ^d   Blin, N ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b Acad Teach Hosp Munich B   (Germany)

^c UNIVERSITY OF MÜNSTER   (Germany)

^d UNIVERSITY OF OSLO   (Norway)

Author keywords

Ancient DNA extracts; Ancient nuclear DNA; Diagenesis; Extract induced mutations; Manganese; PCR errors

Indexed keywords

ADENINE; ANCIENT CELL NUCLEUS DNA; ANCIENT DNA EXTRACT; CELL NUCLEUS DNA; CYTOSINE; DNA; DNA GLYCOSYLTRANSFERASE; GUANINE; MANGANESE CHLORIDE; MITOCHONDRIAL DNA; THYMINE; UNCLASSIFIED DRUG; URACIL; URACIL N GLYCOSYLASE;

ACHONDROPLASIA; ARTICLE; AUTOSOMAL DOMINANT DISORDER; BEAR; CASE REPORT; CONTROLLED STUDY; DIAGENESIS; DNA MODIFICATION; DNA TEMPLATE; EGYPT; ERROR; GENE; GENE AMPLIFICATION; GENE MUTATION; GENE TRANSITION; GENETIC SCREENING; GENOTYPE; GERMANY; HUMAN; HUMAN TISSUE; NONHUMAN; NUCLEOTIDE SEQUENCE; PALEOPATHOLOGY; PENETRANCE; PGFR3 GENE; PHYSICAL ANTHROPOLOGY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SHORT STATURE;

ACHONDROPLASIA; CLONING, MOLECULAR; DNA; DNA GLYCOSYLASES; DNA, COMPLEMENTARY; DNA, MITOCHONDRIAL; EGYPT; EVOLUTION; EVOLUTION, MOLECULAR; GERMANY; HUMANS; MUMMIES; MUTATION; PALEOPATHOLOGY; PHENOTYPE; POINT MUTATION; POLYMERASE CHAIN REACTION; PROTEIN-TYROSINE KINASES; RECEPTOR, FIBROBLAST GROWTH FACTOR, TYPE 3; RECEPTORS, FIBROBLAST GROWTH FACTOR; REPRODUCIBILITY OF RESULTS; SEQUENCE ANALYSIS, DNA; SPECIMEN HANDLING; URACIL-DNA GLYCOSIDASE;

URSUS SPELAEUS;

EID: 6344269077     PISSN: 07374038     EISSN: None     Source Type: Journal    
DOI: 10.1093/molbev/msh208     Document Type: Article

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