Efficient PCR-based random mutagenesis of sub-genic (100 bp) DNA fragments

Yeast

Volumn 14, Issue 1, 1998, Pages 89-91

  Svetlov, Vladimir ^a   Cooper, Terrance G ^a  

^a UNIVERSITY OF TENNESSEE HEALTH SCIENCE CENTER   (United States)

Author keywords

Functional domains; Mutagenesis protocol; PCR mutagenesis; Subgenic DNA fragments

Indexed keywords

DNA FRAGMENT;

CONTROLLED STUDY; MUTAGENESIS; NONHUMAN; NUCLEIC ACID BASE SUBSTITUTION; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SHORT SURVEY; YEAST;

DNA, FUNGAL; GENES, FUNGAL; MUTAGENESIS; POLYMERASE CHAIN REACTION;

EID: 0032518821     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(19980115)14:1<89::AID-YEA194>3.0.CO;2-3     Document Type: Short Survey

References (6)

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3. Hoffman, C. S. and Winston, F. (1987). A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene 57, 267-272.
4. Siderovski, D. P., Matsuyama, T., Frigerio, E., et al. (1992). Random mutagenesis of the human immunodeficiency virus type-1 trans-activator of transcription (HIV-1 Tat). Nucl. Acids Res. 20, 5311-5320.
5. Spee, J. H., de Vos, W. M. and Kuipers, O. P. (1993). Efficient random mutagenesis with adjustable mutation frequency by use of PCR and dITP. Nucl. Acids Res. 21, 777-778.
6. Zhou, Y., Zhang, X. and Ebright, R. H. (1991). Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase. Nucl. Acids Res. 19, 6052.