Pseudomonas aeruginosa-induced infection and degradation of human wound fluid and skin proteins ex vivo are eradicated by a synthetic cationic polymer

Journal of Antimicrobial Chemotherapy

Volumn 54, Issue 4, 2004, Pages 772-779

  Werthen M ^a   Davoudi, M ^b   Sonesson, A ^b   Nitsche, D P ^b   Morgelin M ^b   Blom, K ^a   Schmidtchen, Artur ^b  

^a Molnlycke Health Care   (Sweden)

^b LUND UNIVERSITY   (Sweden)

Author keywords

Antimicrobials; Bacteria; Polyhexamethylenebiguanide; Proteolysis; Wound healing

Indexed keywords

COSMOCIL; ELASTASE; POLY(HEXAMETHYLENEBIGUANIDE);

ARTICLE; BACTERIAL GROWTH; BACTERICIDAL ACTIVITY; BACTERIUM ISOLATE; CONTROLLED STUDY; DIFFUSION; DRUG SCREENING; DRUG STRUCTURE; EXPERIMENTAL INFECTION; GRAM NEGATIVE INFECTION; HUMAN; HUMAN TISSUE; MICROSCOPY; PLASMA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PSEUDOMONAS AERUGINOSA; SCANNING ELECTRON MICROSCOPY; SECRETIONS; SKIN; WOUND;

ANTIMICROBIAL CATIONIC PEPTIDES; BIGUANIDES; COLONY COUNT, MICROBIAL; EXUDATES AND TRANSUDATES; HUMANS; MODELS, BIOLOGICAL; PROTEINS; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; SKIN; WOUND INFECTION;

EID: 6344244516     PISSN: 03057453     EISSN: None     Source Type: Journal    
DOI: 10.1093/jac/dkh407     Document Type: Article

References (28)

1. Steiner, H., Hultmark, D., Engström, A. et al. (1981). Sequence and specificity of two antibacterial proteins involved in insect immunity. Nature 292, 246-8.
2. Selsted, M. E. & Quellette, A. J. (1995). Defensins in granules of phagocytic and non-phagocytic cells. Trends in Cell Biology 5, 114-9.
3. Schröder, J. M. & Harder, J. (1999). Human β-defensin-2. International Journal of Biochemistry and Cell Biology 31, 645-51.
4. Lehrer, R. I. & Ganz, T. (1999). Antimicrobial peptides in mammalian and insect host defence. Current Opinion in Immunology 11, 23-7.
5. Boman, H. G. (2000). Innate immunity and the normal microflora. Immunological Reviews 173, 5-16.
6. Gennaro, R. & Zanetti, M. (2000). Structural features and biological activities of the cathelicidin-derived antimicrobial peptides. Biopolymers 55, 31-49.
7. Zasloff, M. (2002). Antimicrobial peptides of multicellular organisms. Nature 415, 389-95.
8. Boman, H. G. (2003). Antibacterial peptides: basic facts and emerging concepts. Journal of Internal Medicine 254, 197-215.
9. Ong, P. Y., Ohtake, T., Brandt, C. et al. (2002). Endogenous antimicrobial peptides and skin infections in atopic dermatitis. New England Journal of Medicine 347, 1151-60.
10. Pütsep, K., Carlsson, G., Boman, H. G. et al. (2002). Deficiency of antibacterial peptides in patients with morbus Kostmann: an observation study. Lancet 360, 1144-9.
11. Schmidtchen, A., Frick, I. M., Andersson, E. et al. (2002). Proteinases of common pathogenic bacteria degrade and inactivate the antibacterial peptide LL-37. Molecular Microbiology 46, 157-68.
12. Islam, D., Bandholtz, L., Nilsson, J. et al. (2001). Downregulation of bactericidal peptides in enteric infections: a novel immune escape mechanism with bacterial DNA as a potential regulator. Nature Medicine 7, 180-5.
13. Sajjan, U. S., Tran, L. T., Sole, N. et al. (2001). P-113D, an antimicrobial peptide active against Pseudomonas aeruginosa, retains activity in the presence of sputum from cystic fibrosis patients. Antimicrobial Agents and Chemotherapy 45, 3437-44.
14. Fernandez-Lopez, S., Kim, H. S., Choi, E. C. et al. (2001). Antibacterial agents based on the cyclic D,L-α-peptide architecture. Nature 412, 452-5.
15. Broxton, P., Woodcock, P. M., Heatley, F. et al. (1984). Interaction of some polyhexamethylene biguanides and membrane phospholipids in Escherichia coli. Journal of Applied Bacteriology 57, 115-24.
16. Heilborn, J. D., Nilsson, M. F., Kratz, G. et al. (2003). The cathelicidin anti-microbial peptide LL-37 is involved in re-epithelialization of human skin wounds and is lacking in chronic ulcer epithelium. Journal of Investigative Dermatology 120, 379-89.
17. Schmidtchen, A., Holst, E., Tapper, H. et al. (2003). Elastase-producing Pseudomonas aeruginosa degrade plasma proteins and extracellular products of human skin and fibroblasts, and inhibit fibroblast growth. Microbial Pathogenesis 34, 47-55.
18. Schmidtchen, A., Wolff, H. & Hansson, C. (2001). Differential proteinase expression by Pseudomonas aeruginosa derived from chronic leg ulcers. Acta Dermato-Venereologica 81, 406-9.
19. Schmidtchen, A., Frick, I. M. & Björck, L. (2001). Dermatan sulphate is released by proteinases of common pathogenic bacteria and inactivates antibacterial α-defensin. Molecular Microbiology 39, 708-13.
20. Grinnell, F. & Zhu, M. (1996). Fibronectin degradation in chronic wounds depends on the relative levels of elastase, α 1-proteinase inhibitor, and α2-macroglobulin. Journal of Investigative Dermatology 106, 335-41.
21. Lehrer, R. I., Rosenman, M., Harwig, S. S. et al. (1991). Ultrasensitive assays for endogenous antimicrobial polypeptides. Journal of Immunological Methods 137, 167-73.
22. Aszodi, A., Chan, D., Hunziker, E. et al. (1998). Collagen II is essential for the removal of the notochord and the formation of intervertebral discs. Journal of Cell Biology 143 1399-412.
23. Oren, Z., Lerman, J. C., Gudmundsson, G. H. et al. (1999). Structure and organization of the human antimicrobial peptide LL-37 in phospholipid membranes: relevance to the molecular basis for its non-cell-selective activity. Biochemical Journal 341, 501-13.
24. Jones, M. V., Herd, T. M. & Christie, H. J. (1989). Resistance of Pseudomonas aeruginosa to amphoteric and quaternary ammonium biocides. Microbios 58, 49-61.
25. Gilbert, P., Das, J. R., Jones, M. V. et al. (2001). Assessment of resistance towards biocides following the attachment of microorganisms to, and growth on, surfaces. Journal of Applied Microbiology 91, 248-54.
26. Larkin, D. F., Kilvington, S. & Dart, J. K. (1992). Treatment of Acanthamoeba keratitis with polyhexamethylene biguanide. Ophthalmology 99, 185-91.
27. Hansson, C., Hoborn, J., Möller, A. et al. (1995). The microbial flora in venous leg ulcers without clinical signs of infection. Repeated culture using a validated standardised microbiological technique. Acta Dermato-Venereologica 75, 24-30.
28. Davies, C. E., Wilson, M. J., Hill, K. E. et al. (2001). Use of molecular techniques to study microbial diversity in the skin: chronic wounds reevaluated. Wound Repair and Regeneration 9, 332-40.