Characterization of intrinsic efflux activity of enterococcus faecalis ATCC29212 by a semi-automated ethidium bromide method

In Vivo

Volumn 23, Issue 1, 2009, Pages 81-88

  Spengler, Gabriella ^a   Martins, Ana ^a   Schelz, Zsuzsnna ^b   Rodrigues, Liliana ^a   Aagaard, Lone ^c   Martins, Marta ^a   Santos Costa, Sofia ^a   Couto, Isabel ^a,d   Vivieros, Miguel ^a   Fanning, Séamus ^e   Kristiansen, Jette E ^c,f   Molnar, Joseph ^b,f   Amaral, Leonard ^a,f  

^a INSTITUTO DE HIGIENE E MEDICINA TROPICAL   (Portugal)

^b UNIVERSITY OF SZEGED   (Hungary)

^c UNIVERSITY OF SOUTHERN DENMARK   (Denmark)

^d UNIVERSIDADE NOVA DE LISBOA   (Portugal)

^e TRINITY COLLEGE DUBLIN   (Ireland)

^f Cost Action BM0701 ^*  (Portugal)

Author keywords

ABC transporters; Efflux pump inhibitors (EPIs); PH; Proton motive force; Semi automated EB method

Indexed keywords

CARBONYL CYANIDE CHLOROPHENYLHYDRAZONE; ETHIDIUM BROMIDE; PROTON; RESERPINE; THIORIDAZINE;

ANALYTIC METHOD; ANTIBIOTIC RESISTANCE; ARTICLE; CONTROLLED STUDY; EFFLUX ACTIVITY; ENTEROCOCCUS FAECALIS; GLYCOLYSIS; MICROBIAL ACTIVITY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PH;

CARBONYL CYANIDE M-CHLOROPHENYL HYDRAZONE; CELL SURVIVAL; DRUG RESISTANCE, MULTIPLE, BACTERIAL; ENTEROCOCCUS FAECALIS; ETHIDIUM; GLUCOSE; MEMBRANE TRANSPORT PROTEINS; MICROBIAL SENSITIVITY TESTS; RESERPINE; THIORIDAZINE; UNCOUPLING AGENTS;

EID: 63049100884     PISSN: 0258851X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. Fernández Guerrero ML, Goyenechea A, Verdejo C, Roblas RF and de Górgolas M: Enterococcal endocarditis on native and prosthetic valves: a review of clinical and prognostic factors with emphasis on hospital-acquired infections as a major determinant of outcome. Medicine 86: 363-377, 2007.
2. Linden PK and Miller CB: Vancomycin-resistant enterococci: the clinical effect of a common nosocomial pathogen. Diagn Microbiol Infect Dis 33: 113-120, 1999.
3. Rice LB: Antimicrobial resistance in gram-positive bacteria. Am J Med 119: S11-19; S62-70, 2006.
4. Moellering RC Jr: Vancomycin-resistant enterococci. Clin Infect Dis 26: 1196-1199, 1998.
5. Shepard BD and Gilmore MS: Antibiotic-resistant enterococci: the mechanisms and dynamics of drug introduction and resistance. Microbes Infect 4: 215-224, 2002.
6. Franz CM, Stiles ME, Schleifer KH and Holzapfel WH: Enterococci in foods - a conundrum for food safety. Int J Food Microbiol 88: 105-122, 2003.
7. Levy SB: Active efflux mechanisms for antimicrobial resistance. Antimicrob Agents Chemother 36: 695-703, 1992.
8. Lewis K: In search of natural substrates and inhibitors of MDR pumps. J Mol Microbiol Biotechnol 3: 247-254, 2001.
9. Saier MH Jr, Tam R, Reizer A and Reizer J: Two novel families of bacterial membrane proteins concerned with nodulation, cell division and transport. Mol Microbiol 11: 841-847, 1994.
10. Kristiansen JE, Hendricks O, Delvin T, Butterworth TS, Aagaard L, Christensen JB, Flores VC and Keyzer H: Reversal of resistance in microorganisms by help of non-antibiotics. Antimicrob Chemother 59: 1271-1279, 2007.
11. Davis DR, McAlpine JB, Pazoles CJ, Talbot MK, Alder EA, White C, Jonas BM, Murray BE, Weinstock GM and Rogers BL: Enterococcus faecalis multi-drug resistance transporters: application for antibiotic discovery. J Mol Microbiol Biotechnol 3: 179-184, 2001.
12. Jonas BM, Murray BE and Weinstock GM: Characterization of eme A, a NorA homolog and multidrug resistance efflux pump, in Enterococcus faecalis. Antimicrob Agents Chemother 45: 3574-3579, 2001.
13. Lee EW, Chen J, Huda MN, Kuroda T, Mizushima T and Tsuchiya T: Functional cloning and expression of eme A, and characterization of EmeA, a multidrug efflux pump from Enterococcus faecalis. Biol Pharm Bull 26: 266-270, 2003.
14. Lee EW, Huda MN, Kuroda T, Mizushima T and Tsuchiya T: EfrAB, an ABC multidrug efflux pump in Enterococcus faecalis. Antimicrob Agents Chemother 47: 3733-3738, 2003.
15. Putman M, van Veen HW and Konings WN: Molecular properties of bacterial multidrug transporters. Microbiol Mol Biol Rev 64: 672-693, 2000.
16. Viveiros M, Martins A, Paixão L, Rodrigues L, Martins M, Couto I, Fähnrich E, Kern WV and Amaral L: Demonstration of intrinsic efflux activity of Escherichia coli K-12 AG100 by an automated ethidium bromide method. Int J Antimicrob Agents 31: 458-462, 2008.
17. Sharples D, Spengler G, Molnár J, Antal Z, Molnár A, Kiss JT, Szabó JA, Hilgeroth A, Gallo S, Mahamoud A and Barbe J: The interaction between resistance modifiers such as pyrido[3,2-γ]quinoline, aza-oxafluorene and pregnane derivatives with DNA, plasmid DNA and tRNA. Eur J Med Chem 40: 195-202, 2005.
18. Viveiros M, Dupont M, Rodrigues L, Couto I, Davin-Regli A, Martins M, Pagès JM and Amaral L: Antibiotic stress, genetic response and altered permeability of E. coli. PLOS ONE 2(4): e365, 2007.
19. Kristiansen MM, Leandro C, Ordway D, Martins M, Viveiros M, Pacheco T, Molnar J, Kristiansen JE and Amaral L: Thioridazine reduces resistance of methicillin-resistant Staphylococcus aureus by inhibiting a reserpine-sensitive efflux pump. In Vivo 20: 361-366, 2006.
20. Martins A, Couto I, Aagaard L, Martins M, Viveiros M, Kristiansen JE and Amaral L: Prolonged exposure of methicillin-resistant Staphylococcus aureus (MRSA) COL strain to increasing concentrations of oxacillin results in a multidrug-resistant phenotype. Int J Antimicrob Agents 29: 302-305, 2007.
21. Amaral L, Engi H, Viveiros M and Molnar J: Comparison of multidrug-resistant efflux pumps of cancer and bacterial cells with respect to the same inhibitory agents. In Vivo 21: 237-244, 2007.
22. Khan S and Macnab RN: Proton chemical potential, proton electrical potential and bacterial motility. J Mol Biol 138: 599-614, 1980.
23. Giraud E, Cloeckaert A, Kerboeuf D and Chaslus-Dancla E: Evidence for active efflux as the primary mechanism of resistance to ciprofloxacin in Salmonella enterica serovar typhimurium. Antimicrob Agents Chemother 44: 1223-1228, 2000.
24. Magnet S, Courvalin P and Lambert T: Resistance-nodulation-cell division-type efflux pump involved in aminoglycoside resistance in Acinetobacter baumannii strain BM4454. Antimicrob Agents Chemother 45: 3375-3380, 2001.
25. Piddock LJ, Jin YF, Webber MA and Everett MJ: Novel ciprofloxacin-resistant, nalidixic acid-susceptible mutant of Staphylococcus aureus. Antimicrob Agents Chemother 46: 2276-2278, 2002.
26. Couto I, Costa SS, Viveiros M, Martins M and Amaral L: Efflux-mediated response of Staphylococcus aureus exposed to ethidium bromide. J Antimicrob Chemother 62(3): 504-13, 2008.
27. Rodrigues L, Wagner D, Viveiros M, Sampaio D, Couto I, Vavra M, Kern WV and Amaral L: Thioridazine and chlorpromazine inhibition of ethidium bromide efflux in Mycobacterium avium and Mycobacterium smegmatis. J Antimicrob Chemother 61: 1076-1082, 2008.
28. Worley MJ, Stojiljkovic I and Heffron F: The identification of exported proteins with gene fusions to invasin. Mol Microbiol 29: 1471-1480, 1998.
29. Kaatz GW, Moudgal VV, Seo SM and Kristiansen JE: Phenothiazines and thioxanthenes inhibit multidrug efflux pump activity in Staphylococcus aureus. Antimicrob Agents Chemother 47: 719-726, 2003.
30. Schrier RW: Diseases of the Kidney and Urinary Tract. 8th ed. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wilkins, 2006.
31. Zhu H, Hart CA, Sales D and Roberts NB: Bacterial killing in gastric juice - effect of pH and pepsin on Escherichia coli and Helicobacter pylori. J Med Microbiol 55: 1265-1270, 2006.