TtgV Bound to a Complex Operator Site Represses Transcription of the Promoter for the Multidrug and Solvent Extrusion TtgGHI Pump

Journal of Bacteriology

Volumn 186, Issue 10, 2004, Pages 2921-2927

  Guazzaroni, María Eugenia ^a   Terán, Wilson ^a   Zhang, Xiaodong ^b   Gallegos, María Trinidad ^a   Ramos, Juan L ^a,c  

^a CSIC   (Spain)

^b IMPERIAL COLLEGE LONDON   (United Kingdom)

^c CSIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ALKANOL; ANTIBIOTIC AGENT; CARRIER PROTEIN; GENE PRODUCT; GLYCOPROTEIN P; RNA POLYMERASE; SOLVENT; TTGV PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; GENE MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FUNCTION; PROTEIN SECRETION; PSEUDOMONAS PUTIDA; REPRESSOR GENE; TANDEM REPEAT; TRANSCRIPTION REGULATION; TTGGHI OPERON;

BACTERIAL PROTEINS; BINDING SITES; HEXANOLS; MEMBRANE TRANSPORT PROTEINS; MUTAGENESIS, SITE-DIRECTED; PROMOTER REGIONS (GENETICS); PSEUDOMONAS PUTIDA; REPRESSOR PROTEINS;

NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS PUTIDA;

EID: 2342553880     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.10.2921-2927.2004     Document Type: Article

References (31)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1994. Current protocols in molecular biology. John Wiley & Sons, Inc., New York, N.Y.
2. Duque, E., A. Segura, G. Mosqueda, and J. L. Ramos. 2001. Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida. Mol. Microbiol. 39:1100-1106.
3. 54-dependent tandem promoters according to the aromatic compound used for growth. J. Bacteriol. 178:2356-2361.
4. Geanacopoulos, M., and S. Adhya. 2002. Genetic analysis of GalR tetramerization in DNA looping during repressosome assembly. J. Biol. Chem. 277:33148-33152.
5. González-Pérez, M. M., J. L. Ramos, M. T. Gallegos, and S. Marqués. 1999. Critical nucleotides in the upstream region of the XylS-dependent TOL meta-cleavage pathway operon promoter as deduced from analysis of mutants. J. Biol. Chem. 274:2286-2290.
6. Gottesman, M. M., and I. Pastam. 1993. Biochemistry of multidrug resistance mediated by the multidrug transporter. Annu. Rev. Biochem. 62:385-427.
7. Heldwein, E. E., and R. G. Brennan. 2001. Crystal structure of the transcription activator BmrR bound to DNA and a drug. Nature 409:378-382.
8. Koronakis, V., A. Sharff, E. Koronakis, B. Luisi, and C. Hughes. 2001. Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature 405:914-919.
9. Lisser, S., and H. Margalit. 1993. Compilation of E. coli mRNA promoter sequences. Nucleic Acids Res. 21:1507-1516.
10. Maloy, S. R., and W. D. Dunn. 1982. Genetic regulation of the glyoxylate shunt in Escherichia coli K-12. J. Bacteriol. 149:173-180.
11. Marqués, S., J. L. Ramos, and K. N. Timmis. 1993. Analysis of the mRNA structure of the Pseudomonas putida TOL meta-fission pathway operon. Biochim. Biophys. Acta 1216:227-236.
12. Mosqueda, G., and J. L. Ramos. 2000. A set of genes encoding a second toluene efflux system in Pseudomonas putida DOT-TIE is linked to the tod genes for toluene metabolism. J. Bacteriol. 182:937-943.
13. Murakami, S., R. Nakashima, E. Yamashita, and A. Yamaguchi. 2002. Crystal structure of bacterial multidrug efflux transporter AcrB. Nature 419:587-593.
14. Nasser, W., S. Reverchon, and J. Robert-Baudouy. 1992. Purification and functional characterization of the KdgR protein, a major repressor of pectinolysis genes of Erwinia chrysanthemi. Mol. Microbiol. 6:257-265.
15. Nikaido, H. 1996. Multidrug efflux pumps of gram-negative bacteria. J. Bacteriol. 178:5853-5859.
16. Nikaido, H. 1998. Multiple antibiotic resistance and efflux. Curr. Opin. Microbiol. 1:516-523.
17. Poole, K. 2000. Efflux-mediated resistance to fluoroquinolones in gram-negative bacteria. Antimicrob. Agents Chemother. 44:2233-2241.
18. Poole, K. 2000. Efflux-mediated resistance to fluoroquinolones in gram-positive bacteria and the mycobacteria. Antimicrob. Agents Chemother. 44:2595-2599.
19. Ramos, J. L., E. Duque, M. T. Gallegos, P. Godoy, M. I. Ramos-González A. Rojas, W. Terán, and A. Segura. 2002. Mechanisms of solvent tolerance in gram-negative bacteria. Annu. Rev. Microbiol. 56:743-768.
20. Ramos, J. L., E. Duque, P. Godoy, and A. Segura. 1998. Efflux pumps involved in toluene tolerance in Pseudomonas putidu DOT-TIE. J. Bacteriol. 180:3323-3329.
21. Ramos, J. L., E. Duque, M.-J. Huertas, A. Haïdour. 1995. Isolation and expansion of the catabolic potential of a Pseudomonas putida strain able to grow in the presence of high concentrations of aromatic hydrocarbons. J. Bacteriol. 177:3911-3916.
22. Rojas, A., A. Segura, M. E. Guazzaroni, W. Terán, A. Hurtado, M. T. Gallegos, and J. L. Ramos. 2003. In vivo and in vitro evidence show that TtgV is the specific regulator of the TtgGHI multidrug and solvent efflux pump of Pseudomonas putida. J. Bacteriol. 185:4755-4763.
23. Rojas, A., E. Duque, G. Mosqueda, G. Golden, A. Hurtado, J. L. Ramos, and A. Segura. 2001. Three efflux pumps are required to provide efficient tolerance to toluene in Pseudomonas putida DOT-TIE. J. Bacteriol. 183:3967-3973.
24. Rojo, F. 1999. Repression of transcription initiation in bacteria. J. Bacteriol. 181:2987-2991.
25. Saier, J., and I. Paulsen. 2001. Phylogeny of multidrug transporters. Cell Dev. Biol. 12:205-213.
26. Spaink, H. P., R. J. H. Okker, C. A., Wijffelman, E. Pees, and B. Lugtenberg. 1987. Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI. Plant Mol. Biol. 9:27-39.
27. Sunnaborg, A., D. Klumpp, T. Chung, and D. C. LaPorte. 1990. Regulation of the glyoxylate bypass operon: cloning and characterization of iclR. J. Bacteriol. 172:2642-2649.
28. Terán, W., A. Felipe, A. Segura, A. Rojas, J. L. Ramos, and M. T. Gallegos. 2003. Antibiotic-dependent induction of Pseudomonas putida DOT-TIE TtgABC efflux pump is mediated by the drug binding repressor TtgR. Antimicrob. Agents Chemother. 47:3067-3072.
29. Yu, E. W., J. R. Aires, and H. Nikaido. 2003. AcrB multidrug efflux pump of Escherichia coli: composite substrate-binding cavity of exceptional flexibility generates its extremely wide substrate specificity. J. Bacteriol. 185:5657-5664.
30. Yu, E. W., G. McDermott, H. I. Zgurskaya, H. Nikaido, and D. E. Koshland, Jr. 2003. Structural basis of multiple drug-binding capacity of the AcrB multidrug efflux pump. Science 300:976-980.
31. Zgurskaya, H. I., and H. Nikaido. 1999. AcrA is a highly asymmetric monomeric protein capable of spanning the periplasm. J. Mol. Biol. 285:409-420.