Complexity in efflux pump control: Cross-regulation by the paralogues TtgV and TtgT

Molecular Microbiology

Volumn 66, Issue 6, 2007, Pages 1416-1428

  Terán, Wilson ^a   Felipe, Antonia ^a   Fillet, Sandy ^a   Guazzaroni, María Eugenia ^a   Krell, Tino ^a   Ruiz, Raquel ^a   Ramos, Juan L ^a   Gallegos, María Trinidad ^a  

^a CSIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; ORGANIC SOLVENT; TTGABC PROTEIN; TTGGH PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; CHROMOSOME ANALYSIS; GENE CONTROL; GENE EXPRESSION; NONHUMAN; OPEN READING FRAME; OPERATOR GENE; OPERON; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN TRANSPORT; PSEUDOMONAS PUTIDA; PUMP;

BACTERIAL PROTEINS; DNA FOOTPRINTING; ELECTROPHORETIC MOBILITY SHIFT ASSAY; GENE EXPRESSION REGULATION, BACTERIAL; MEMBRANE TRANSPORT PROTEINS; MODELS, GENETIC; OPERON; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PSEUDOMONAS PUTIDA; REPRESSOR PROTEINS; STYRENE;

PSEUDOMONAS PUTIDA;

EID: 36549075919     PISSN: 0950382X     EISSN: 13652958     Source Type: Journal    
DOI: 10.1111/j.1365-2958.2007.06004.x     Document Type: Article

References (46)

1. Aharoni, A., Gaidukov, L., Khersonsky, O., McQ Gould, S., Roodveldt, C. Tawfik, D.S. (2005) The 'evolvability' of promiscuous protein functions. Nat Genet 37 : 73 76.
2. Alekshun, M.N. Levy, S.B. (1997) Regulation of chromosomally mediated multiple antibiotic resistance: the mar regulon. Antimicrob Agents Chemother 41 : 2067 2075.
3. Ausubel, F.M., Brent, R., Kingston, R.F., Moore, D.D., Seidman, J.G., Smith, J.A. Struhl, K. (1991) Current Protocols in Molecular Biology. New York : Greene Publishing Associated.
4. Dagley, S. (1971) Catabolism of aromatic compounds by micro-organisms. Adv Microb Physiol 6 : 1 46.
5. Duque, E., Segura, A., Mosqueda, G. Ramos, J.L. (2001) Global and cognate regulators control the expression of the organic solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida. Mol Microbiol 39 : 1100 1106.
6. Enderle, P.J. Farwell, M.A. (1998) Electroporation of freshly plated Escherichia coli and Pseudomonas aeruginosa cells. Biotechniques 25 : 954 956.
7. Fellay, R., Frey, J. Krisch, H. (1987) Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. Gene 52 : 147 154.
8. Guazzaroni, M.E., Terán, W., Zhang, X., Gallegos, M.T. Ramos, J.L. (2004) TtgV bound to a complex operator site represses transcription of the promoter for the multidrug and solvent extrusion TtgGHI pump. J Bacteriol 186 : 2921 2927.
9. Guazzaroni, M.E., Krell, T., Felipe, A., Ruiz, R., Meng, C., Zhang, X., et al. (2005) The multidrug efflux regulator TtgV recognizes a wide range of structurally different effectors in solution and complexed with target DNA: evidence from isothermal titration calorimetry. J Biol Chem 280 : 20887 20893.
10. Heipieper, H.J. de Bont, J.A.M. (1994) Adaptation of Pseudomonas putida S12 to ethanol and toluene at the level of fatty acid composition of membranes. App Environ Microbiol 60 : 4440 4444.
11. Heipieper, H.J., Menlenbeld, G., van Oirschot, Q. de Bont, J.A.M. (1996) Effect of environmental factors on the cis/trans ratio of unsaturated fatty acids in Pseudomonas S12. Appl Environ Microbiol 62 : 2773 2777.
12. Hillen, W. Berens, C. (1994) Mechanisms underlying expression of Tn10 encoded tetracycline resistance. Annu Rev Microbiol 48 : 345 369.
13. Inoue, A. Horikoshi, K. (1989) A Pseudomonas thrives in high concentrations of toluene. Nature 338 : 264 266.
14. Isken, S. de Bont, J.A.M. (1996) Active efflux of toluene in a solvent-resistant bacterium. J Bacteriol 178 : 6056 6058.
15. Jude, F., Arpin, C., Brachet-Castang, C., Capdepuy, M., Caumette, P. Quentin, C. (2004) TbtABM, a multidrug efflux pump associated with tributyltin resistance in Pseudomonas stutzeri. FEMS Microbiol Lett 232 : 7 14.
16. Junker, F. Ramos, J.L. (1999) Involvement of the cis-trans isomerase CtiT1 in solvent resistance in Pseudomonas putida DOT T1. J Bacteriol 181 : 5693 5700.
17. Kobayashi, H., Uematsu, K., Hirayama, H. Horikoshi, K. (2000) Novel toluene elimination system in a toluene-tolerant microorganism. J Bacteriol 182 : 6451 6455.
18. Krell, T., Molina-Henares, A.J. Ramos, J.L. (2006) The IclR family of transcriptional activators and repressors can be defined by a single profile. Protein Sci 15 : 1207 1213.
19. Loffeld, B. Keweloh, H. (1996) Cis/trans Isomerization of unsaturated fatty acids as possible control mechanism of membrane fluidity in Pseudomonas putida P8. Lipids 31 : 811 815.
20. Ma, D., Cook, D.N., Alberti, M., Pon, N.G., Nikaido, H. Hearst, J.E. (1995) Genes acrA and acrB encode a stress-induced efflux system of Escherichia coli. Mol Microbiol 16 : 45 55.
21. Ma, D., Alberti, M., Lynch, C., Nikaido, H. Hearst, J.E. (1996) The local repressor AcrR plays a modulating role in the regulation of acrAB genes of Escherichia coli by global stress signals. Mol Microbiol 19 : 101 112.
22. McAdams, H.H. Arkin, A. (1997) Stochastic mechanisms in gene expression. Proc Natl Acad Sci USA 94 : 814 819.
23. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory.
24. Miller, P.F. Sulavik, M.C. (1996) Overlaps and parallels in the regulation of intrinsic multiple-antibiotic resistance in Escherichia coli. Mol Microbiol 21 : 441 448.
25. Molina-Henares, J., Krell, T., Guazzaroni, M.E., Segura, A. Ramos, J.L. (2006) Members of the IclR family of bacterial transcriptional regulators function as activators and/or repressors. FEMS Microbiol Rev 30 : 157 186.
26. Mosqueda, G. Ramos, J.L. (2000) A set of genes encoding a second toluene efflux system in Pseudomonas putida DOT-T1E is linked to the tod genes for toluene metabolism. J Bacteriol 182 : 937 943.
27. Nies, D.H., Rehbein, G., Hoffmann, T., Baumann, C. Grosse, C. (2006) Paralogs of genes encoding metal resistance proteins in Cupriavidus metallidurans strain CH34. J Mol Microbiol Biotechnol 11 : 82 93.
28. Okusu, H., Ma, D. Nikaido, H. (1996) AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic- resistance (Mar) mutants. J Bacteriol 178 : 306 308.
29. Phoenix, P., Keane, A., Patel, A., Bergeron, H., Ghoshal, S. Lau, P.C. (2003) Characterization of a new solvent-responsive gene locus in Pseudomonas putida F1 and its functionalization as a versatile biosensor. Environ Microbiol 5 : 1309 1327.
30. Ramos, J.L., Duque, E., Huertas, M.J. Haidour, A. (1995) Isolation and expansion of the catabolic potential of a Pseudomonas putida strain able to grow in the presence of high concentrations of aromatic hydrocarbons. J Bacteriol 177 : 3911 3916.
31. Ramos, J.L., Duque, E., Godoy, P. Segura, A. (1998) Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-T1E. J Bacteriol 180 : 3323 3329.
32. Ramos, J.L., Duque, E., Gallegos, M.T., Godoy, P., Ramos-González, M.I., Rojas, A., et al. (2002) Mechanisms of solvent tolerance in gram-negative bacteria. Annu Rev Microbiol 56 : 743 768.
33. Ramos, J.L., Martínez-Bueno, M., Molina-Henares, A.J., Terán, W., Watanabe, K., Zhang, X., et al. (2005) The TetR family of transcriptional repressors. Microbiol Mol Biol Rev 69 : 326 356.
34. Ramos-González, M.I., Duque, E. Ramos, J.L. (1991) Conjugational transfer of recombinant DNA in cultures and in soils: host range of Pseudomonas putida TOL plasmids. J Bacteriol 57 : 3020 3027.
35. Rodríguez-Herva, J.J., García, V., Hurtado, A., Segura, A. Ramos, J.L. (2007) The ttgGHI solvent efflux pump operon of Pseudomonas putida DOT-T1E is located on a large self-transmissible plasmid. Environ Microbiol 9 : 1550 1561.
36. Rojas, A., Duque, E., Mosqueda, G., Golden, G., Hurtado, A., Ramos, J.L. Segura, A. (2001) Three efflux pumps are required to provide efficient tolerance to toluene in Pseudomonas putida DOT-T1E. J Bacteriol 183 : 3967 3973.
37. Rojas, A., Segura, A., Guazzaroni, M.E., Terán, W., Hurtado, A., Gallegos, M.T. Ramos, J.L. (2003) In vivo and in vitro evidence shows that TtgV is the local specific regulator of the TtgGHI multidrug and solvent efflux pump of Pseudomonas putida. J Bacteriol 185 : 4755 4763.
38. Rosenfeld, N., Elowitz, M.B. Alon, U. (2002) Negative autoregulation speeds the response times of transcription networks. J Mol Biol 323 : 785 793.
39. Sánchez-Romero, J.M., Díaz-Orejas, R. de Lorenzo, V. (1998) Resistance to tellurite as a selection marker for genetic manipulations of Pseudomonas strains. Appl Environ Microbiol 64 : 4040 4046.
40. Segura, A., Rojas, A., Hurtado, A., Huertas, M.J. Ramos, J.L. (2003) Comparative genomic analysis of solvent extrusion pumps in Pseudomonas strains exhibiting different degrees of solvent tolerance. Extremophiles 7 : 371 376.
41. Sikkema, J., de Bont, J.A.M. Poolman, B. (1995) Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev 59 : 201 222.
42. Spaink, H.P., Okker, R.J.H., Wijffelman, C.A., Pees, E. Lugtenberg, B.J.J. (1987) Promoters in the nodulation region of the Rhizobium leguminosarum Sym plasmid pRL1JI. Plant Mol Biol 9 : 27 39.
43. Terán, W., Felipe, A., Segura, A., Rojas, A., Ramos, J.L. Gallegos, M.T. (2003) Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux pump is mediated by the drug binding repressor TtgR. Antimicrob Agents Chemother 47 : 3067 3072.
44. Terán, W., Krell, T., Ramos, J.L. Gallegos, M.T. (2006) Effector-repressor interactions, binding of a single effector molecule to the operator-bound TtgR homodimer mediates derepression. J Biol Chem 281 : 7102 7109.
45. Wery, J., Hidayat, B., Kieboom, J. de Bont, J.A. (2001) An insertion sequence prepares Pseudomonas putida S12 for severe solvent stress. J Biol Chem 276 : 5700 5706.
46. White, D.G., Goldman, J.D., Demple, B. Levy, S.B. (1997) Role of the acrAB locus in organic solvent tolerance mediated by expression of marA, soxS, or robA in Escherichia coli. J Bacteriol 179 : 6122 6126.