Effects of temperature on the detection of methicillin resistance in Staphylococcus aureus using cefoxitin disc diffusion testing with Iso-Sensitest agar

Journal of Antimicrobial Chemotherapy

Volumn 63, Issue 4, 2009, Pages 699-703

  Skov, R ^a   Smyth, R ^b   Yusof, A ^c   Karlsson, A ^c   Mills, K ^c   Frimodt Moller, N ^a   Kahlmeter, G ^b,d  

^a STATENS SERUM INSTITUT   (Denmark)

^b CENTRAL HOSPITAL   (Sweden)

^c AB BIODISK   (Sweden)

^d UPPSALA UNIVERSITY   (Sweden)

Author keywords

MRSA; S. aureus; Susceptibility testing

Indexed keywords

AGAR; CEFOXITIN; METICILLIN; OXACILLIN; PENICILLIN BINDING PROTEIN 2A;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CONTROLLED STUDY; DISK DIFFUSION; INCUBATION TEMPERATURE; INCUBATION TIME; INOCULATION; METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; RELIABILITY; TIME;

AGAR; ANTI-BACTERIAL AGENTS; CEFOXITIN; CULTURE MEDIA; HUMANS; METHICILLIN RESISTANCE; MICROBIAL SENSITIVITY TESTS; STAPHYLOCOCCUS AUREUS; TEMPERATURE; TIME FACTORS;

EID: 62549102470     PISSN: 03057453     EISSN: 14602091     Source Type: Journal    
DOI: 10.1093/jac/dkp019     Document Type: Article

References (14)

1. Grundmann H, Aires-de-Sousa M, Boyce J et al. Emergence and resurgence of methicillin-resistant Staphylococcus aureus as a public-health threat. Lancet 2006; 368: 874-85.
2. Andrews JM. BSAC standardized disc susceptibility testing method (version 7). J Antimicrob Chemother 2007; 60: 20-41.
3. Olsson-Liljequist B, Larsson P, Walder M et al. Antimicrobial susceptibility testing in Sweden. III. Methodology for susceptibility testing. Scand J Infect Dis Suppl 1997; 105: 13-23.
4. Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Disk Susceptibility Tests - Ninth Edition: Approved Standard M2-A9. CLSI, Wayne, PA, USA, 2006.
5. Skov R, Larsen AR, Frimodt-Moller N et al. Evaluation of different disk diffusion/media combinations for detection of methicillin resistance in Staphylococcus aureus and coagulase-negative staphylococci. APMIS 2003; 111: 905-14.
6. Andrews J, Brenwald N, Brown DF et al. Evaluation of a 10 μg cefoxitin disc for the detection of methicillin resistance in Staphylococcus aureus by BSAC methodology. J Antimicrob Chemother 2005; 56: 599-600.
7. Felten A, Grandry B, Lagrange PH et al. Evaluation of three techniques for detection of low-level methicillin-resistant Staphylococcus aureus (MRSA): A disk diffusion method with cefoxitin and moxalactam, the Vitek 2 system, and the MRSA-screen latex agglutination test. J Clin Microbiol 2002; 40: 2766-71.
8. Skov R, Smyth R, Larsen AR et al. Evaluation of cefoxitin 5 and 10 μg discs for the detection of methicillin resistance in staphylococci. J Antimicrob Chemother 2005; 55: 157-61.
9. Skov R, Smyth R, Clausen M et al. Evaluation of a cefoxitin 30 μg disc on Iso-Sensitest agar for detection of methicillin-resistant Staphylococcus aureus. J Antimicrob Chemother 2003; 52: 204-7.
10. Skov R, Smyth R, Larsen AR et al. Phenotypic detection of methicillin resistance in Staphylococcus aureus by disk diffusion testing and Etest on Mueller-Hinton agar. J Clin Microbiol 2006; 44: 4395-9.
11. Swenson JM, Lonsway D, McAllister S et al. Detection of mecA mediated resistance using reference and commercial testing methods in a collection of Staphylococcus aureus expressing borderline oxacillin MICs. Diagn Microbiol Infect Dis 2007; 58: 33-9.
12. The Swedish Reference Group for Antibiotics (SRGA) and its Subcommittee on Methodology (SRGA-M). Methicillin Resistance. www.SRGA.org (8 November 2008, date last accessed).
13. Cauwelier B, Gordts B, Descheemaecker P et al. Evaluation of a disk diffusion method with cefoxitin (30 μg) for detection of methicillin-resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis 2004; 23: 389-92.
14. Skov RL, Pallesen LV, Poulsen RL et al. Evaluation of a new 3-h hybridization method for detecting the mecA gene in Staphylococcus aureus and comparison with existing genotypic and phenotypic susceptibility testing methods. J Antimicrob Chemother 1999; 43: 467-75.