Evaluation of a new 3-h hybridization method for detecting the mecA gene in Staphylococcus aureus and comparison with existing genotypic and phenotypic susceptibilty testing methods

Journal of Antimicrobial Chemotherapy

Volumn 43, Issue 4, 1999, Pages 467-475

  Skov, Robert L ^a   Pallesen, Lars V ^a   Poulsen, Rikke L ^a   Espersen, Frank ^a  

^a STATENS SERUM INSTITUT   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; METICILLIN; OXACILLIN;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; DNA HYBRIDIZATION; GENOTYPE; INTERMETHOD COMPARISON; NONHUMAN; PENICILLIN RESISTANCE; PHENOTYPE; STAPHYLOCOCCUS AUREUS;

EID: 0032928527     PISSN: 03057453     EISSN: None     Source Type: Journal    
DOI: 10.1093/jac/43.4.467     Document Type: Article

References (33)

1. Hiramatsu, K. (1995). Molecular evolution of MRSA. Microbiology and Immunology 39, 531-43.
2. Tomasz, A., Drugeon, H. B., de Lencastre, H. M., Jabes, D., McDougall, L. & Bille, J. (1989). New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP 2a gene and contain normal penicillin-binding proteins with modified penicillin-binding capacity. Antimicrobial Agents and Chemotherapy 33, 1869-74.
3. Henze, U. U. & Berger-Bachi, B. (1996). Penicillin-binding protein 4 overproduction increases β-lactam resistance in Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 40, 2121-5.
4. Matthews, P. R. & Stewart, P. R. (1984). Resistance heterogeneity in methicillin-resistant Staphylococcus aureus. FEMS Microbiology Letters 22, 161-6.
5. Chambers, H. F. (1997). Methicillin resistance in staphylococci: molecular and biochemical basis and clinical implications. Clinical Microbiology Reviews 10, 781-91.
6. Bignardi, G. E., Woodford, N., Chapman, A., Johnson, A. P. & Speller, D. C. E. (1996). Detection of the mec-A gene and phenotypic detection of resistance in Staphylococcus aureus isolates with borderline or low-level methicillin resistance. Journal of Antimicrobial Chemotherapy 37, 53-63.
7. Hartman, B. J. & Tomasz, A. (1986). Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 29, 85-92.
8. Skulnick, M., Simor, A. E., Gregson, D., Patel, M., Small, G. W., Kreiswirth, B. et al. (1992). Evaluation of commercial and standard methodology for determination of oxacillin susceptibility in Staphylococcus aureus. Journal of Clinical Microbiology 30, 1985-8.
9. Mackenzie, A. M., Richardson, H., Lannigan, R. & Wood, D. (1995). Evidence that the National Committee for Clinical Laboratory Standards disk test is less sensitive than the screen plate for detection of low-expression-class methicillin-resistant Staphylococcus aureus. Journal of Clinical Microbiology 33, 1909-11.
10. Archer, G. L. & Pennell, E. (1990). Detection of methicillin resistance in staphylococci by using a DNA probe. Antimicrobial Agents and Chemotherapy 34, 1720-4.
11. Youmans, G. R., Davis, T. E. & Fuller, D. D. (1993). Use of chemiluminescent DNA probes in the rapid detection of oxacillin resistance in clinically isolated strains of Staphylococcus aureus. Diagnostic Microbiology and Infectious Disease 16, 99-104.
12. Kolbert, C. P., Connolly, J. E., Lee, M. J. & Persing, D. H. (1995). Detection of the staphylococcal mecA gene by chemiluminescent DNA hybridization. Journal of Clinical Microbiology 33, 2179-82.
13. Vannuffel, P., Gigi, J., Ezzedine, H., Vandercam, B., Delmee, M., Wauters, G. et al. (1995). Specific detection of methicillin-resistant Staphylococcus species by multiplex PCR. Journal of Clinical Microbiology 33, 2864-7.
14. Geha, D. J., Uhl, J. R., Gustaferro, C. A. & Persing, D. H. (1994). Multiplex PCR for identification of methicillin-resistant staphylococci in the clinical laboratory. Journal of Clinical Microbiology 32, 1768-72.
15. Hiramatsu, K., Kihara, H. & Yokota, T. (1992). Analysis of borderline-resistant strains of methicillin-resistant Staphylococcus aureus using polymerase chain reaction. Microbiology and Immunology 36, 445-53.
16. Ubukata, K., Nakagami, S., Nitta, A., Yamane, A., Kawakami, S., Sugiura, M. et al. (1992). Rapid detection of the mecA gene in methicillin-resistant staphylococci by enzymatic detection of polymerase chain reaction products. Journal of Clinical Microbiology 30, 1728-33.
17. Unal, S., Hoskins, J., Flokowitsch, J. E., Wu, C. Y. E., Preston, D. A. & Skatrud, P. L. (1992). Detection of methicillin-resistant staphylococci by using the polymerase chain reaction. Journal of Clinical Microbiology 30, 1685-91.
18. Ryffel, C., Tesch, W., Birch-Machin, I., Reynolds, P. E., Barberis-Maino, L., Kayser, F. H. et al. (1990). Sequence comparison of mecA genes isolated from methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis. Gene 94, 137-8.
19. Shortle, D. (1983). A genetic system for analysis of staphylococcal nuclease. Gene 22, 181-9.
20. National Committee for Clinical Laboratory Standards. (1997). Performance Standards for Antimicrobial Disk Susceptibility Tests -Sixth Edition: Approved Standard M2-A6. NCCLS, Wayne, PA.
21. National Committee for Clinical Laboratory Standards. (1997). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically - Fourth Edition: Approved Standard M7-A4. NCCLS, Wayne, PA.
22. Olsson-Liljequist, B., Larsson, P., Walder, M. & Miörner, H. (1997). Antimicrobial susceptibility testing in Sweden. III. Methodology for susceptibility testing. Scandinavian Journal of Infectious Diseases Suppl. 105, 13-23.
23. Alfa, M. J., Ongsansoy, E., Hoban, S., Mathew, T. & Greenaway, C. (1994). Methicillin-resistant Staphylococcus aureus. Optimization of the agar screen plate method. Diagnostic Microbiology and Infectious Disease 18, 121-4.
24. Voss, A. & Doebbeling, B. N. (1995). The worldwide prevalence of methicillin-resistant Staphylococcus aureus. International Journal of Antimicrobial Agents 5, 101-6.
25. Jones, R. N., Marshall, S. A., Pfaller, M. A., Wilke, W. W., Hollis, R. J., Erwin, M. E. et al. (1997). Nosocomial enterococcal blood stream infections in the SCOPE program: antimicrobial resistance, species occurrence, molecular testing results, and laboratory testing accuracy. Diagnostic Microbiology and Infectious Disease 29, 95-102.
26. Hiramatsu, K., Hanaki, H., Ino, T., Yabuta, K., Oguri, T. & Tenover, F. C. (1997). Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility. Journal of Antimicrobial Chemotherapy 40, 135-6.
27. Gerberding, J. L., Miick, C., Liu, H. H. & Chambers, H. F. (1991). Comparison of conventional susceptibility tests with direct detection of penicillin-binding protein 2a in borderline oxacillin-resistant strains of Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 35, 2574-9.
28. Frimodt-Moller, N., Rosdahl, V. T., Sorensen, G., Hartzen, S. H. & Bentzon, M. W. (1986). Relationship between penicillinase production and the in-vitro activity of methicillin, oxacillin, cloxacillin, dicloxacillin, flucloxacillin, and cephalothin against strains of Staphylococcus aureus of different phage patterns and penicillinase activity. Journal of Antimicrobial Chemotherapy 18, 27-33.
29. Kotilainen, P., Hyvarinen, J., Jarvinen, H., Linko, L., Eerola, E., Lehtonen, O. P. et al. (1995). Testing of methicillin resistance by in vitro susceptibility and the presence of the mecA gene in clinical Staphylococcus aureus isolates in Finland. Scandinavian Journal of Infectious Diseases 27, 475-9.
30. Huang, M. B., Gay, T. E., Baker, C. N., Banerjee, S. N. & Tenover, F. C. (1993). Two per cent sodium chloride is required for susceptibility testing of staphylococci with oxacillin when using agar-based dilution methods. Journal of Clinical Microbiology 31, 2683-8.
31. de Lencastre, H., Sá Figueiredo, A. M., Urban, C., Rahal, J. & Tomasz, A. (1991). Multiple mechanisms of methicillin resistance and improved methods for detection in clinical isolates of Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 35, 632-9.
32. McMurray, L. W., Kernodle, D. S. & Barg, N. L. (1990). Characterization of a widespread strain of methicillin-susceptible Staphylococcus aureus associated with nosocomial infections. Journal of Infectious Diseases 162, 759-62.
33. Woods, G. L. & Yam, P. (1988). Bactericidal activity of oxacillin against β-lactamase hyperproducing Staphylococcus aureus. Antimicrobial Agents and Chemotherapy 32, 1614-8.