Regulation of streptokinase expression by CovR/S in Streptococcus pyogenes: CovR acts through a single high-affinity binding site

Microbiology

Volumn 155, Issue 2, 2009, Pages 566-575

  Churchward, Gordon ^a   Bates, Christopher ^a   Gusa, Asiya A ^a   Stringert, Virginia ^a,b   Scott, June R ^a  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b ATLANTA VA MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; PROTEIN COVR; STREPTOKINASE; UNCLASSIFIED DRUG; COVS PROTEIN, STREPTOCOCCUS PYOGENES; CSRR PROTEIN, STREPTOCOCCUS PYOGENES; REPRESSOR PROTEIN; SIGNAL PEPTIDE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; GENE REPRESSION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; STREPTOCOCCUS PYOGENES; TRANSCRIPTION REGULATION; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION;

STREPTOCOCCUS PYOGENES; STREPTOCOCCUS SP. 'GROUP A';

BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PROTEIN BINDING; REPRESSOR PROTEINS; STREPTOCOCCUS PYOGENES; STREPTOKINASE;

EID: 62249188955     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.024620-0     Document Type: Article

References (47)

1. Ashbaugh, C. D., Moser, T. J., Shearer, M. H., White, G. L., Kennedy, R. C. & Wessels, M. R. (2000). Bacterial determinants of persistent throat colonization and the associated immune response in a primate model of human group A streptococcal pharyngeal infection. Cell Microbiol 2, 283-292.
2. Brinkmann, V., Reichard, U., Goosmann, C., Fauler, B., Uhlemann, Y., Weiss, D. S., Weinrauch, Y. & Zychlinsky, A. (2004). Neutrophil extracellular traps kill bacteria. Science 303, 1532-1535.
3. Buchanan, J. T., Simpson, A. J., Aziz, R. K., Liu, G. Y., Kristian, S. A., Kotb, M., Feramisco, J. & Nizet, V. (2006). DNase expression allows the pathogen group A streptococcus to escape killing in neutrophil extracellular traps. Curr Biol 16, 396-400.
4. Churchward, G. (2007). The two faces of Janus: virulence gene regulation by CovR/S in group A streptococci. Mol Microbiol 64, 34-41.
5. Cole, J. N., McArthur, J. D., McKay, F. C., Sanderson-Smith, M. L., Cork, A. J., Ranson, M., Rohde, M., Itzek, A., Sun, H. & other authors (2006). Trigger for group A streptococcal M1T1 invasive disease. FASEB J 20, 1745-1747.
6. Collin, M. & Olsen, A. (2001). Effect of SpeB and EndoS from Streptococcus pyogenes on human immunoglobulins. Infect Immun 69, 7187-7189.
7. Cunningham, M. W. (2000). Pathogenesis of Group A streptococcal infection. Clin Microbiol Rev 13, 470-511.
8. Dalton, T. L. & Scott, J. R. (2004). CovS inactivates CovR and is required for growth under conditions of general stress in Streptococcus pyogenes. J Bacteriol 186, 3928-3937.
9. Dalton, T. L., Collins, J. T., Barnett, T. C. & Scott, J. R. (2006). RscA, a member of the MDR1 family of transporters, is repressed by CovR and required for growth of Streptococcus pyogenes under heat stress. J Bacteriol 188, 77-85.
10. Federle, M. J. & Scott, J. R. (2002). Identification of binding sites for the group A streptococcal global regulator CovR. Mol Microbiol 43, 1161-1172.
11. Federle, M. J., McIver, K. S. & Scott, J. R. (1999). A response regulator that represses transcription of several virulence operons in the group A streptococcus. J Bacteriol 181, 3649-3657.
12. Frank, C., Steiner, K. & Malke, H. (1995). Conservation of the organization of the streptokinase gene region among pathogenic streptococci. Med Microbiol Immunol 184, 139-146.
13. Gao, J., Gusa, A. A., Scott, J. R. & Churchward, G. (2005). Binding of the global response regulator protein CovR to the sag promoter of Streptococcus pyogenes reveals a new mode of CovR-DNA interaction. J Biol Chem 280, 38948-38956.
14. Gase, K., Ellinger, T. & Malke, H. (1995). Complex transcriptional control of the streptokinase gene of Streptococcus equisimilis H46A. Mol Gen Genet 247, 749-758.
15. Geist, R. T., Okada, N. & Caparon, M. G. (1993). Analysis of Streptococcus pyogenes promoters by using novel Tn916-based shuttle vectors for the construction of transcriptional fusions to chloramphenicol acetyltransferase. J Bacteriol 175, 7561-7570.
16. Graham, M. R., Smoot, L. M., Migliaccio, C. A., Virtaneva, K., Sturdevant, D. E., Porcella, S. F., Federle, M. J., Adams, G. J., Scott, J. R. & Musser, J. M. (2002). Virulence control in group A streptococcus by a two-component gene regulatory system: global expression profiling and in vivo infection modeling. Proc Natl Acad Sci U S A 99, 13855-13860.
17. Graham, M. R., Virtaneva, K., Porcella, S. F., Barry, W. T., Gowen, B. B., Johnson, C. R., Wright, F. A. & Musser, J. M. (2005). Group A streptococcus transcriptome dynamics during growth in human blood reveals bacterial adaptive and survival strategies. Am J Pathol 166, 455-465.
18. Graham, M. R., Virtaneva, K., Porcella, S. F., Gardner, D. J., Long, R. D., Welty, D. M., Barry, W. T., Johnson, C. A., Parkins, L. D. & other authors (2006). Analysis of the transcriptome of group A streptococcus in mouse soft tissue infection. Am J Pathol 169, 927-942.
19. Gusa, A. A. & Scott, J. R. (2005). The CovR response regulator of group A streptococcus (GAS) acts directly to repress its own promoter. Mol Microbiol 56, 1195-1207.
20. Gusa, A. A., Gao, J., Stringer, V., Churchward, G. & Scott, J. R. (2006). Phosphorylation of the group A streptococcal CovR response regulator causes dimerization and promoter-specific recruitment by RNA polymerase. J Bacteriol 188, 4620-4626.
21. Heath, A., DiRita, V. J., Barg, N. L. & Engleberg, N. C. (1999). A two-component regulatory system, CsrR-CsrS, represses expression of three Streptococcus pyogenes virulence factors, hyaluronic acid capsule, streptolysin S, and pyrogenic exotoxin B. Infect Immun 67, 5298-5305.
22. Husmann, L. K., Yung, D. L., Hollingshead, S. K. & Scott, J. R. (1997). Role of putative virulence factors of Streptococcus pyogenes in mouse models of long-term throat colonization and pneumonia. Infect Immun 65, 1422-1430.
23. Kreikemeyer, B., Boyle, M. D., Buttaro, B. A., Heinemann, M. & Podbielski, A. (2001). Group A streptococcal growth phase-associated virulence factor regulation by a novel operon (Fas) with homologies to two-component-type regulators requires a small RNA molecule. Mol Microbiol 39, 392-406.
24. Kwinn, L. A. & Nizet, V. (2007). How group A streptococcus circumvents host phagocyte defenses. Future Microbiol 2, 75-84.
25. Laub, M. T. & Goulian, M. (2007). Specificity in two-component signal transduction pathways. Annu Rev Genet 41, 121-145.
26. Levin, J. C. & Wessels, M. R. (1998). Identification of csrR/csrS, a genetic locus that regulates hyaluronic acid capsule synthesis in group A streptococcus. Mol Microbiol 30, 209-219.
27. Malke, H., Steiner, K., Gase, K. & Frank, C. (2000). Expression and regulation of the streptokinase gene. Methods 21, 111-124.
28. Martinez-Hackert, E. & Stock, A. M. (1997). The DNA-binding domain of OmpR: crystal structures of a winged helix transcription factor. Structure 5, 109-124.
29. Miller, A. A., Engleberg, N. C. & DiRita, V. J. (2001). Repression of virulence genes by phosphorylation-dependent oligomerization of CsrR at target promoters in S. pyogenes. Mol Microbiol 40, 976-990.
30. Munson, G. P. & Scott, J. R. (1999). Binding site recognition by Rns, a virulence regulator in the AraC family. J Bacteriol 181, 2110-2117.
31. Musser, J. M. & DeLeo, F. R. (2005). Toward a genome-wide systems biology analysis of host-pathogen interactions in group A streptococcus. Am J Pathol 167, 1461-1472.
32. Nordstrand, A., McShan, W. M., Ferretti, J. J., Holm, S. E. & Norgren, M. (2000). Allele substitution of the streptokinase gene reduces the nephritogenic capacity of group A streptococcal strain NZ131. Infect Immun 68, 1019-1025.
33. Opdyke, J. A., Scott, J. R. & Moran, C. P., Jr (2001). A secondary RNA polymerase sigma factor from Streptococcus pyogenes. Mol Microbiol 42, 495-502.
34. Rhee, J. E., Sheng, W., Morgan, L. K., Nolet, R., Liao, X. & Kenney, L. J. (2008). Amino acids important for DNA recognition by the response regulator OmpR. J Biol Chem 283, 8664-8677.
35. Roberts, S. A. & Scott, J. R. (2007). RivR and the small RNA RivX: the missing links between the CovR regulatory cascade and the Mga regulon. Mol Microbiol 66, 1506-1522.
36. Roberts, S. A., Churchward, G. G. & Scott, J. R. (2007). Unraveling the regulatory network in Streptococcus pyogenes: the global response regulator CovR represses rivR directly. J Bacteriol 189, 1459-1463.
37. Salim, K. Y., de Azavedo, J. C., Bast, D. J. & Cvitkovitch, D. G. (2007). Role for sagA and siaA in quorum sensing and iron regulation in Streptococcus pyogenes. Infect Immun 75, 5011-5017.
38. Scott, J. R. (1972). A new gene controlling lysogeny in phage P1. Virology 48, 282-283.
39. Scott, J. R., Guenthner, P. C., Malone, L. M. & Fischetti, V. A. (1986). Conversion of an M- group A streptococcus to M+ by transfer of a plasmid containing an M6 gene. J Exp Med 164, 1641-1651.
40. Stock, A. M., Robinson, V. L. & Goudreau, P. N. (2000). Two-component signal transduction. Annu Rev Biochem 69, 183-215.
41. Sumby, P., Barbian, K. D., Gardner, D. J., Whitney, A. R., Welty, D. M., Long, R. D., Bailey, J. R., Parnell, M. J., Hoe, N. P. & other authors (2005). Extracellular deoxyribonuclease made by group A streptococcus assists pathogenesis by enhancing evasion of the innate immune response. Proc Natl Acad Sci U S A 102, 1679-1684.
42. Sumby, P., Whitney, A. R., Graviss, E. A., DeLeo, F. R. & Musser, J. M. (2006). Genome-wide analysis of group A streptococci reveals a mutation that modulates global phenotype and disease specificity. PLoS Pathog 2, e5.
43. Sun, H., Ringdahl, U., Homeister, J. W., Fay, W. P., Engleberg, N. C., Yang, A. Y., Rozek, L. S., Wang, X., Sjöbring, U. & Ginsburg, D. (2004). Plasminogen is a critical host pathogenicity factor for group A streptococcal infection. Science 305, 1283-1286.
44. Tart, A. H., Walker, M. J. & Musser, J. M. (2007). New understanding of the group A streptococcus pathogenesis cycle. Trends Microbiol 15, 318-325.
45. Virtaneva, K., Porcella, S. F., Graham, M. R., Ireland, R. M., Johnson, C. A., Ricklefs, S. M., Babar, I., Parkins, L. D., Romero, R. A. & other authors (2005). Longitudinal analysis of the group A streptococcus transcriptome in experimental pharyngitis in cynomolgus macaques. Proc Natl Acad Sci U S A 102, 9014-9019.
46. Walker, M. J., Hollands, A., Sanderson-Smith, M. L., Cole, J. N., Kirk, J. K., Henningham, A., McArthur, J. D., Dinkla, K., Aziz, R. K. & other authors (2007). DNase Sda1 provides selection pressure for a switch to invasive group A streptococcal infection. Nat Med 13, 981-985.
47. Wessels, M. R. & Bronze, M. S. (1994). Critical role of the group A streptococcal capsule in pharyngeal colonization and infection in mice. Proc Natl Acad Sci U S A 91, 12238-12242.