Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase

Applied and Environmental Microbiology

Volumn 75, Issue 6, 2009, Pages 1758-1763

  Yanai, Hisaaki ^a   Doi, Katsumi ^a   Ohshima, Toshihisa ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DEHYDROGENASE; MEDIUM-CHAIN ALCOHOLS; RECOMBINANT ENZYMES; SULFOLOBUS TOKODAII;

ESCHERICHIA COLI;

ENZYMES;

ALCOHOL; ENZYME ACTIVITY; GENE EXPRESSION; GENOME; PH; PROKARYOTE; RECOMBINATION; TOLERANCE;

ALCOHOL DEHYDROGENASE; ARCHAEAL PROTEINS; CLONING, MOLECULAR; COENZYMES; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; NAD; RECOMBINANT PROTEINS; SULFOLOBUS;

ESCHERICHIA COLI; SULFOLOBUS TOKODAII;

EID: 62149103359     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01392-08     Document Type: Article

References (25)

1. Ajay, A. K., and D. N. Srivastava. 2007. Microtubular conductometric biosensor for ethanol detection. Biosens. Bioelectron. 23:281-284.
2. Antoine, E., J. L. Rolland, J. P. Raffin, and J. Dietrich. 1999. Cloning and over-expression in Escherichia coli of the gene encoding NADPH group 111 alcohol dehydrogenase from Thermococcus hydrothermalis. Characterization and comparison of the native and the recombinant enzymes. Eur. J. Biochem. 264:880-889.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Cannio, R., G. Fiorentino, P. Carpinelli, M. Rossi, and S. Bartolucci. 1996. Cloning and overexpression in Escherichia coli of the genes encoding NAD-dependent alcohol dehydrogenase from two Sulfolobus species. J. Bacteriol. 178:301-305.
5. Davis, B. J, 1964. Disc electrophoresis. II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci. 121:404-427.
6. de Miguel Bouzas, T., J. Barros-Velazquez, and T. G. Villa. 2006. Industrial applications of hyperthermophilic enzymes: a review. Protein Pept. Lett. 13:645-651.
7. Egorova, K., and G. Antranikian. 2005. Industrial relevance of thermophilic archaea. Curr. Opin. Microbiol. 8:649-655.
8. Esposito, L., F. Sica, C. A. Raia, A. Giordano, M. Rossi, L. Mazzarella, and A. Zagari. 2002. Crystal structure of the alcohol dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus at 1.85 A resolution. J. Mol. Biol. 318:463-477.
9. Giordano, A., R. Cannio, F. La Cara, S. Bartolucci, M. Rossi, and C. A. Raia. 1999. Asn249Tyr substitution at the coenzyme binding domain activates Sulfolobus solfataricus alcohol dehydrogenase and increases its thermal stability. Biochemistry 38:3043-3054.
10. Hirakawa, H., N. Kamiya, Y, Kawarabayashi, and T. Nagamune, 2004. Properties of an alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix Kl. J. Biosci. Bioeng. 97:202-206.
11. Hummel, W. 1997. New alcohol dehydrogenases for the synthesis of chiral compounds. Adv. Biochem. Eng. Biotechnol. 58:145-184.
12. Jornvall, H., B. Persson, and J. Jeffery. 1987. Characteristics of alcohol/polyol dehydrogenases. The zinc-containing long-chain alcohol dehydrogenases. Eur. J. Biochem. 167:195-201.
13. Kawarabayasi, Y., Y. Hino, H. Horikawa, K. Jin-no, M. Takahashi, M. Sekine, S, Baba, A. Ankai, H, Kosugi, A, Hosoyama, S. Fukui, Y. Nagai, K. Nishijima, R. Otsuka, H. Nakazawa, M. Takamiya, Y. Kato, T. Yoshizawa, T. Tanaka, Y. Kudoh, J. Yamazaki, N. Kushida, A. Oguchi, K. Aoki, S. Masuda, M. Yanagii, M, Nishimura, A, Yamagishi, T. Oshima, and H, Kikuchi. 2001. Complete genome sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7. DNA Res. 8:123-140.
14. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
15. Larkin, M. A., G. Blackshields, N. P. Brown, R. Chenna, P. A. MeGettigan, H. McWilliam, F. Valentin, I. M. Wallace, A. Wilm, R. Lopez, J. D. Thompson, T. J. Gibson, and D. G. Higgins. 2007. Clustal W and Clustal X version 2.0. Bioinformatics 23:2947-2948.
16. Li, D., and K. J. Stevenson, 1997. Purification and sequence analysis of a novel NADP(H)-dependent type III alcohol dehydrogenase from Thermococcus strain AN1. J. Bacteriol. 179:4433-4437.
17. Ma, K., and M. W. Adams. 1999. An unusual oxygen-sensitive, iron- and zinc-containing alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus. J. Bacteriol. 181:1163-1170.
18. Ma, K., F. T, Robb, and M. W. Adams. 1994. Purification and characterization of NADP-specific alcohol dehydrogenase and glutamate dehydrogenase from the hyperthermophilic archaeon Thermococcus litoralis. Appl. Environ. Microbiol. 60:562-568.
19. Ma, K., H. Loessner, J. Heider, M. K. Johnson, and M. W. Adams. 1995. Effects of elemental sulfur on the metabolism of the deep-sea hyperthermophilic archaeon Thermococcus strain ES-1: characterization of a sulfur-regulated, non-heme iron alcohol dehydrogenase. J. Bacteriol. 177:4748-4756.
20. Machielsen, R., A. R. Uria, S. W. Kengen, and J. van der Oost. 2006. Production and characterization of a thermostable alcohol dehydrogenase that belongs to the aldo-keto reductase superfamily. Appl. Environ. Microbiol. 72:233-238.
21. Raia, C. A., A. Giordano, and M. Rossi. 2001. Alcohol dehydrogenase from Sulfolobus solfataricus. Methods Enzymol. 331:176-195.
22. Raia, C. A., C. Caruso, M. Marino, N. Vespa, and M. Rossi. 1996. Activation of Sulfolobus solfataricus alcohol dehydrogenase by modification of cysteine residue 38 with iodoacetic acid. Biochemistry 35:638-647.
23. +-dependent alcohol dehydrogenase. Purification and properties. Eur. J. Biochem. 167:475-479.
24. Rudge, J., and G. F. Bickerstaff. 1986. Purification and properties of an alcohol dehydrogenase from Sporotrichum pulverulentum. Enzyme Microb. Technol. 8:120-124.
25. van der Oost, J., W. G. Voorhorst, S. W. Kengen, A. C. Geerling, V. Wittenhorst, Y. Gueguen, and W. M. de Vos. 2001. Genetic and biochemical characterization of a short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus. Eur. J. Biochem. 268:3062-3068.