Hairy-root organ cultures for the production of human acetylcholinesterase

BMC Biotechnology

Volumn 8, Issue , 2008, Pages

  Woods, Ryan R ^a   Geyer, Brian C ^a   Mor, Tsafrir S ^a  

^a ARIZONA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL CULTURE; CHEMICAL WARFARE; CHROMATOGRAPHIC ANALYSIS; ENZYMES; MAMMALS; PLANTS (BOTANY);

ACETYLCHOLINESTERASE; AGROBACTERIUM RHIZOGENES; BIPHASIC; ELECTROPHORETIC HOMOGENEITIES; ENZYMATIC PROPERTIES; EXPRESSION LEVELS; HAIRY ROOT CULTURES; HAIRY ROOTS; HUMAN ENZYMES; MAMMALIAN CELL CULTURES; NERVE AGENTS; NICOTIANA BENTHAMIANA; ORGAN CULTURES; PHARMACOKINETIC PROPERTIES; PLANT MATERIALS; PLANT ORGANS; PRODUCTION SYSTEMS; ROOT CULTURES; ROOT-ORGAN CULTURES; STATIONARY PHASE; TOTAL SOLUBLE PROTEINS; TRANSGENIC; TRANSGENIC PLANTS; WILD-TYPE CONTROLS;

CELL CULTURE;

ACETYLCHOLINESTERASE; RECOMBINANT PROTEIN;

ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; ELECTROPHORESIS; ENZYME PURIFICATION; ENZYME SYNTHESIS; HAIRY ROOT CULTURE; HUMAN; MALE; MOUSE; NICOTIANA BENTHAMIANA; NONHUMAN; PLANT CELL CULTURE; PLANT GROWTH; PROTEIN EXPRESSION; RHIZOBIUM RHIZOGENES; TOBACCO; TRANSGENIC PLANT; ANIMAL; BIOSYNTHESIS; CELL CULTURE; ENZYMOLOGY; GENE TRANSFER; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; PLANT ROOT;

AGROBACTERIUM RHIZOGENES; MAMMALIA; NICOTIANA BENTHAMIANA;

ACETYLCHOLINESTERASE; ANIMALS; CELLS, CULTURED; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HUMANS; MALE; MICE; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; TOBACCO;

EID: 61649119662     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-8-95     Document Type: Article

References (24)

1. Ashani Y Prospective of human butyrylcholinesterase as a detoxifying antidote and potential regulator of controlled-release drugs Drug Dev Res 2000, 50(3-4):298-308. 10.1002/1098-2299(200007/08)50:3/ 4<298::AID-DDR13>3.0.CO;2-X
2. Doctor BP Saxena A Bioscavengers for the protection of humans against organophosphate toxicity Chem Biol Interact 2005, 157-158:167-171. 10.1016/j.cbi.2005.10.024
3. Grunwald J Marcus D Papier Y Raveh L Pittel Z Ashani Y Large-scale purification and long-term stability of human butyrylcholinesterase: A potential bioscavenger drug J Biochem Biophys Methods 1997, 34(2):123-135. 10.1016/S0165-022X(97)01208-6 9178088
4. Lenz DE Maxwell DM Koplovitz I Clark CR Capacio BR Cerasoli DM Federko JM Luo C Saxena A Doctor BP et al.: Protection against soman or VX poisoning by human butyrylcholinesterase in guinea pigs and cynomolgus monkeys Chem Biol Interact 2005, 157-158:205-210. 10.1016/ j.cbi.2005.10.025
5. Velan B Kronman C Grosfeld H Leitner M Gozes Y Flashner Y Sery T Cohen S Ben-Aziz R Seidman S et al.: Recombinant human acetylcholinesterase is secreted from transiently transfected 293 cells as a soluble globular enzyme Cellular & Molecular Neurobiology 1991, 11(1):143-156. 10.1007/BF00712806
6. Kronman C Velan B Gozes Y Leitner M Flashner Y Lazar A Marcus D Sery T Papier Y Grosfeld H et al.: Production and secretion of high levels of recombinant human acetylcholinesterase in cultured cell lines: microheterogeneity of the catalytic subunit Gene 1992, 121(2):295-304. 10.1016/0378-1119(92)90134-B 1446827
7. Saxena A Ashani Y Raveh L Stevenson D Patel T Doctor BP Role of oligosaccharides in the pharmacokinetics of tissue-derived and genetically engineered cholinesterases Mol Pharmacol 1998, 53(1):112-122. 9443938
8. Cerasoli DM Griffiths EM Doctor BP Saxena A Fedorko JM Greig NH Yu QS Huang Y Wilgus H Karatzas CN In vitro and in vivo characterization of recombinant human butyrylcholinesterase (Protexia(TM)) as a potential nerve agent bioscavenger Chemico-Biological Interactions 2005, 157-158:362. 10.1016/j.cbi.2005.10.052
9. Mor TS Sternfeld M Soreq H Arntzen CJ Mason HS Expression of recombinant human acetylcholinesterase in transgenic tomato plants Biotechnol Bioeng 2001, 75(3):259-266. 10.1002/bit.10012 11590598
10. Geyer BC Fletcher SP Griffin TA Lopker MJ Soreq H Mor TS Translational control of recombinant human acetylcholinesterase accumulation in plants BMC Biotechnol 2007, 7:27. 1913049 17537261 10.1186/1472-6750-7-27
11. Geyer BC Muralidharan M Cherni I Doran J Fletcher SP Evron T Soreq H Mor TS Purification of Transgenic Plant-Derived Recombinant Human Acetylcholinesterase-R Chem Biol Interact 2005, 157-158:331-334. 10.1016/ j.cbi.2005.10.097
12. Evron T Geyer BC Cherni I Muralidharan M Kilbourne J Fletcher SP Soreq H Mor TS Plant-derived human acetylcholinesterase-R provides protection from lethal organophosphate poisoning and its chronic aftermath Faseb J 2007, 21(11):2961-2969. 10.1096/fj.07-8112com 17475919
13. Streatfield SJ Regulatory issues for plant-made pharmaceuticals and vaccines Expert Rev Vaccines 2005, 4(4):591-601. 10.1586/ 14760584.4.4.591 16117714
14. Sivakumar G Bioreactor technology: A novel industrial tool for high-tech production of bioactive molecules and biopharmaceuticals from plant roots Biotechnol J 2006, 1(12):1419-1427. 10.1002/biot.200600117 17136730
15. Srivastava S Srivastava AK Hairy root culture for mass-production of high-value secondary metabolites Crit Rev Biotechnol 2007, 27(1):29-43. 10.1080/07388550601173918 17364688
16. Guillon S Tremouillaux-Guiller J Pati PK Rideau M Gantet P Hairy root research: Recent scenario and exciting prospects Curr Opin Plant Biol 2006, 9(3):341-346. 10.1016/j.pbi.2006.03.008 16616871
17. Ellman GL Courtney KD Andres V Jr Feather-Stone RM A new and rapid colorimetric determination of acetylcholinesterase activity Biochem Pharmacol 1961, 7:88-95. 10.1016/0006-2952(61)90145-9 13726518
18. Soreq H Seidman S Acetylcholinesterase - new roles for an old actor Nat Rev Neurosci 2001, 2(4):294-302. 10.1038/35067589 11283752
19. Sternfeld M Ming G Song H Sela K Timberg R Poo M Soreq H Acetylcholinesterase enhances neurite growth and synapse development through alternative contributions of its hydrolytic capacity, core protein, and variable C termini J Neurosci 1998, 18(4):1240-1249. 9454834
20. Ordentlich A Barak D Kronman C Ariel N Segall Y Velan B Shafferman A Contribution of aromatic moieties of tyrosine 133 and of the anionic subsite tryptophan 86 to catalytic efficiency and allosteric modulation of acetylcholinesterase J Biol Chem 1995, 270(5):2082-2091. 10.1074/ jbc.270.5.2082 7836436
21. Sharp JM Doran PM Strategies for enhancing monoclonal antibody accumulation in plant cell and organ cultures Biotechnol Prog 2001, 17(6):979-992. 10.1021/bp010104t 11735430
22. Su WW Guan P Bugos RC High-level secretion of functional green fluorescent protein from transgenic tobacco cell cultures: characterization and sensing Biotechnology & Bioengineering 2004, 85(6):610-619. 10.1002/bit.10916
23. Becerra-Arteaga A Mason HS Shuler ML Production, secretion, and stability of human secreted alkaline phosphatase in tobacco NT1 cell suspension cultures Biotechnol Prog 2006, 22(6):1643-1649. 17137313
24. Cohen O Kronman C Raveh L Mazor O Ordentlich A Shafferman A Comparison of polyethylene glycol-conjugated recombinant human acetylcholinesterase and serum human butyrylcholinesterase as bioscavengers of organophosphate compounds Mol Pharmacol 2006, 70(3):1121-1131. 10.1124/ mol.106.026179 16801396