Development of a novel GLUT4 translocation assay for identifying potential novel therapeutic targets for insulin sensitization

Biochemical Journal

Volumn 418, Issue 2, 2009, Pages 413-420

  Liu, Franklin ^a   Dallas Yang, Qing ^a   Castriota, Gino ^a   Fischer, Paul ^a   Santini, Francesca ^a   Ferrer, Marc ^a   Li, Jing ^a   Akiyama, Taro E ^a   Berger, Joel P ^a   Zhang, Bei B ^a   Jiang, Guoqiang ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Diabetes; Glucose transporter 4 (GLUT4); High throughput screening; Insulin receptor substrate 1 (IRS1); Insulin resistance; Therapeutic target

Indexed keywords

DIABETES; GLUCOSE TRANSPORTER 4 (GLUT4); HIGH-THROUGHPUT SCREENING; INSULIN RECEPTOR SUBSTRATE 1 (IRS1); INSULIN RESISTANCE; THERAPEUTIC TARGET;

AMINO ACIDS; ASSAYS; CELL MEMBRANES; ELECTRIC BATTERIES; ENZYME ACTIVITY; ENZYME INHIBITION; FLUORESCENCE; GENE EXPRESSION; GLUCOSE; HIGH TEMPERATURE SUPERCONDUCTORS; NUCLEIC ACIDS; PHOSPHORYLATION; RNA; SUBSTRATES; SUGAR (SUCROSE); TARGETS;

INSULIN;

ENHANCED GREEN FLUORESCENT PROTEIN; GLUCOSE TRANSPORTER 4; INSULIN; INSULIN RECEPTOR SUBSTRATE 1; MYC PROTEIN; PALMITIC ACID; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHATIDYLINOSITOL 3 KINASE INHIBITOR; PHOSPHATIDYLINOSITOL 3,4,5 TRISPHOSPHATE 3 PHOSPHATASE; PROTEIN KINASE B; PROTEIN KINASE B INHIBITOR; PROTEIN KINASE C THETA; PROTEIN TYROSINE PHOSPHATASE 1B; S6 KINASE; SERINE; SMALL INTERFERING RNA; STRESS ACTIVATED PROTEIN KINASE; ANTIDIABETIC AGENT; GLUCOSE; INSULIN RECEPTOR SUBSTRATE; ISOENZYME; PRKCQ PROTEIN, HUMAN; PROTEIN KINASE C; PTEN PROTEIN, HUMAN; SLC2A4 PROTEIN, HUMAN; STRESS ACTIVATED PROTEIN KINASE 1;

ARTICLE; CELL ASSAY; CELL DENSITY; CONTROLLED STUDY; EMBRYO; ENZYME INHIBITION; GENE EXPRESSION; HUMAN; HUMAN CELL; INSULIN RESISTANCE; INSULIN SENSITIVITY; NON INSULIN DEPENDENT DIABETES MELLITUS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN TRANSPORT; SIGNAL TRANSDUCTION; CELL CULTURE; DRUG SCREENING; GENE SILENCING; GENETIC TRANSFECTION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; VALIDATION STUDY;

ANIMALIA;

1-PHOSPHATIDYLINOSITOL 3-KINASE; CELLS, CULTURED; DIABETES MELLITUS, TYPE 2; DRUG EVALUATION, PRECLINICAL; GENE KNOCKDOWN TECHNIQUES; GLUCOSE; GLUCOSE TRANSPORTER TYPE 4; HUMANS; HYPOGLYCEMIC AGENTS; INSULIN; INSULIN RECEPTOR SUBSTRATE PROTEINS; INSULIN RESISTANCE; ISOENZYMES; MITOGEN-ACTIVATED PROTEIN KINASE 8; PALMITIC ACID; PROTEIN KINASE C; PROTEIN TRANSPORT; PTEN PHOSPHOHYDROLASE; RIBOSOMAL PROTEIN S6 KINASES; TRANSFECTION;

EID: 61449130618     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20082051     Document Type: Article

References (24)

1. Huang, S. and Czech, M. P. (2007) The GLUT4 glucose transporter. Cell Metab. 5, 237-252
2. Wallberg-Henriksson, H. and Zierath, J. R. (2001) GLUT4: a key player regulating glucose homeostasis? Insights from transgenic and knockout mice (review). Mol. Membr. Biol. 18, 205-211
3. Carvalho, E., Kotani, K., Peroni, O. D. and Kahn, B. B. (2005) Adipose-specific overexpression of GLUT4 reverses insulin resistance and diabetes in mice lacking GLUT4 selectively in muscle. Am. J. Physiol. Endocrinol. Metab. 289, E551-E561
4. Zisman, A., Peroni, O. D., Abel, E. D., Michael, M. D., Mauvais-Jarvis, F., Lowell, B. B., Wojtaszewski, J. F., Hirshman, M. F., Virkamaki, A., Goodyear, L. J. et al. (2000) Targeted disruption of the glucose transporter 4 selectively in muscle causes insulin resistance and glucose intolerance. Nat. Med. 6, 924-928
5. Kotani, K., Peroni, O. D., Minokoshi, Y., Boss, O. and Kahn, B. B. (2004) GLUT4 glucose transporter deficiency increases hepatic lipid production and peripheral lipid utilization. J. Clin. Invest. 114, 1666-1675
6. Watson, R. T. and Pessin, J. E. (2007) GLUT4 translocation: the last 200 nanometers. Cell Signalling 19, 2209-2217
7. Bryant, N. J., Govers, R. and James, D. E. (2002) Regulated transport of the glucose transporter GLUT4. Nat. Rev. Mol. Cell Biol. 3, 267-277
8. Wang, Q., Khayat, Z., Kishi, K., Ebina, Y. and Klip, A. (1998) GLUT4 translocation by insulin in intact muscle cells: detection by a fast and quantitative assay. FEBS Lett. 427, 193-197
9. Kanai, F., Nishioka, Y., Hayashi, H., Kamohara, S., Todaka, M. and Ebina, Y. (1993) Direct demonstration of insulin-induced GLUT4 translocation to the surface of intact cells by insertion of a c-Myc epitope into an exofacial GLUT4 domain. J. Biol. Chem. 268, 14523-14526
10. Kanai, F., Ito, K., Todaka, M., Hayashi, H., Kamohara, S., Ishii, K., Okada, T., Hazeki, O., Ui, M. and Ebina, Y. (1993) Insulin-stimulated GLUT4 translocation is relevant to the phosphorylation of IRS-1 and the activity of PI3-kinase. Biochem. Biophys. Res. Commun. 195, 762-768
11. De Fea, K. and Roth, R. A. (1997) Modulation of insulin receptor substrate-1 tyrosine phosphorylation and function by mitogen-activated protein kinase. J. Biol. Chem. 272, 31400-31406
12. Jiang, G., Li, Z., Liu, F., Ellsworth, K., Dallas-Yang, Q., Wu, M., Ronan, J., Esau, C., Murphy, C., Szalkowski, D., Bergeron, R., Doebber, T. and Zhang, B. B. (2005) Prevention of obesity in mice by antisense oligonucleotide inhibitors of stearoyl-CoA desaturase-1. J. Clin. Invest. 115, 1030-1038
13. DeFeo-Jones, D., Barnett, S. F., Fu, S., Hancock, P. J., Haskell, K. M., Leander, K. R., McAvoy, E., Robinson, R. G., Duggan, M. E., Lindsley, C. W. et al. (2005) Tumor cell sensitization to apoptotic stimuli by selective inhibition of specific Akt/PKB family members. Mol. Cancer Ther. 4, 271-279
14. Aguirre, V., Werner, E. D., Giraud, J., Lee, Y. H., Shoelson, S. E. and White, M. F. (2001) Phosphorylation of SER307 in IRS-1 blocks interactions with the insulin receptor and inhibits insulin action. J. Biol. Chem. 277, 1531-1517
15. 307. J. Biol. Chem. 275, 9047-9054
16. Bossenmaier, B., Mosthaf, L., Mischak, H., Ullrich, A. and Haring, H. U. (1997) Protein kinase C isoforms beta 1 and beta 2 inhibit the tyrosine kinase activity of the insulin receptor. Diabetologia 40, 863-866
17. De Fea, K. and Roth, R. A. (1997) Protein kinase C modulation of insulin receptor substrate-1 tyrosine phosphorylation requires serine 612. Biochemistry 36, 12939-12947
18. Hotamisligil, G. S., Peraldi, P., Budavari, A., Ellis, R., White, M. F. and Spiegelman, B. M. (1996) IRS-1-mediated inhibition of insulin receptor tyrosine kinase activity in TNF-alpha- and obesity-induced insulin resistance. Science 271, 665-668
19. White, M. F. (2002) IRS proteins and the common path to diabetes. Am. J. Physiol. Endocrinol. Metab. 283, E413-E422
20. Werner, E. D., Lee, J., Hansen, L., Yuan, M. and Shoelson, S. E. (2004) Insulin resistance due to phosphorylation of IRS-1 at serine 302. J. Biol. Chem. 279, 35298-35305
21. Qiao, L. Y., Goldberg, J. L., Russell, J. C. and Sun, X. J. (1999) Identification of enhanced serine kinase activity in insulin resistance. J. Biol. Chem. 274, 10625-10632
22. Lee, Y. H. and White, M. F. (2004) Insulin receptor substrate proteins and diabetes. Arch. Pharm. Res. 27, 361-370
23. Boden, G. and Shulman, G. I. (2002) Free fatty acids in obesity and type 2 diabetes: defining their role in the development of insulin resistance and beta-cell dysfunction. Eur. J. Clin. Invest. 32 (Suppl. 3), 14-23
24. Jiang, G. and Zhang, B. B. (2002) PI 3-kinase and its up- and down-stream modulators as potential targets for the treatment of type II diabetes. Front. Biosci. 7, d903-d907