Comparison of the oxime-induced reactivation of rhesus monkey, swine and guinea pig erythrocyte acetylcholinesterase following inhibition by sarin or paraoxon, using a perfusion model for the real-time determination of membrane-bound acetylcholinesterase activity

Toxicology

Volumn 258, Issue 2-3, 2009, Pages 79-83

  Herkert, Nadja M ^a   Lallement, Guy ^b   Clarençon, Didier ^b   Thiermann, Horst ^a   Worek, Franz ^a  

^a BUNDESWEHR INSTITUTE OF PHARMACOLOGY AND TOXICOLOGY   (Germany)

^b CENTRE DE RECHERCHES   (France)

Author keywords

Acetylcholinesterase; Erythrocyte; Inhibition; Kinetic analysis; Organophosphorus compounds; Reactivation

Indexed keywords

1 (4 CARBAMOYLPYRIDINIO) 1' (2 HYDROXYIMINOMETHYLPYRIDINIO)DIMETHYL ETHER; 5,5' DITHIOBIS(2 NITROBENZOIC ACID); ACETYLCHOLINESTERASE; ACETYLTHIOCHOLINE; OBIDOXIME; PARAOXON; PHOSPHATE BUFFERED SALINE; SARIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME KINETICS; ERYTHROCYTE; EXPERIMENTAL MODEL; FLOW MEASUREMENT; GUINEA PIG; MALE; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; RHESUS MONKEY; SWINE;

ACETYLCHOLINESTERASE; ANIMALS; CHOLINESTERASE INHIBITORS; CHOLINESTERASE REACTIVATORS; ENZYME ACTIVATION; ERYTHROCYTES; GUINEA PIGS; KINETICS; MACACA MULATTA; MALE; OXIMES; PARAOXON; PERFUSION; SARIN; SUS SCROFA;

ANIMALIA; CAVIA; MACACA MULATTA; SUIDAE;

EID: 61349135388     PISSN: 0300483X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tox.2009.01.014     Document Type: Article

References (22)

1. Aurbek N., Thiermann H., Szinicz L., Eyer P., and Worek F. Analysis of inhibition, reactivation and aging kinetics of highly toxic organophosphorus compounds with human and pig acetylcholinesterase. Toxicology 224 (2006) 91-99
2. Dawson R.M. Review of oximes available for treatment of nerve agent poisoning. J. Appl. Toxicol. 14 (1994) 317-331
3. Eckert, S.T., 2006. Entwicklung eines dynamischen Modells zum Studium der Schutzeffekte reversibler Acetylcholinesterase-Hemmstoffe vor der irreversiblen Hemmung durch hochtoxische Organophosphate. Thesis. Ludwigs-Maximilians-Universität München.
4. Eckert S., Eyer P., Mückter H., and Worek F. Development of a dynamic model for real-time determination of membrane-bound acetylcholinesterase activity upon perfusion with inhibitors and reactivators. Biochem. Pharmacol. 72 (2006) 358-365
5. Eckert S., Eyer P., Mückter H., and Worek F. Kinetic analysis of the protection afforded by reversible inhibitors against irreversible inhibition of acetylcholinesterase by highly toxic organophosphorus compounds. Biochem. Pharmacol. 72 (2006) 344-357
6. Eckert S., Eyer P., Herkert N., Bumm R., Weber G., Thiermann H., and Worek F. Comparison of the oxime-induced reactivation of erythrocyte and muscle acetylcholinesterase following inhibition by sarin and paraoxon, using a perfusion model for the real-time determination of membrane-bound acetylcholinesterase activity. Biochem. Pharmacol. 75 (2008) 698-703
7. Eyer P. The role of oximes in the management of organophosphorus pesticide poisoning. Toxicol. Rev. 22 (2003) 165-190
8. Eyer P., and Worek F. Oximes. In: Marrs T.C., Maynard R.L., and Sidell F.R. (Eds). Chemical Warfare Agents: Toxicology and Treatment (2007), John Wiley & Sons Ltd., Chichester 305-329
9. Herkert N., Eckert S., Eyer P., Bumm R., Weber G., Thiermann H., and Worek F. Identical kinetics of human erythrocyte and muscle acetylcholinesterase with respect to carbamate pre-treatment, residual activity upon soman challenge and spontaneous reactivation after withdrawal of the inhibitors. Toxicology 246 (2008) 188-192
10. Kiderlen D., Eyer P., and Worek F. Formation and disposition of diethylphosphoryl-obidoxime, a potent anticholinesterase that is hydrolysed by human paraoxonase (PON1). Biochem. Pharmacol. 69 (2005) 1853-1867
11. Luo C., Tong M., Chilukuri N., Brecht K., Maxwell D.M., and Saxena A. An in vitro comparative study on the reactivation of nerve agent-inhibited guinea pig and human acetylcholinesterases by oximes. Biochemistry 46 (2007) 11771-11779
12. Luo C., Tong M., Maxwell D.M., and Saxena A. Comparison of oxime reactivation and aging of nerve agent-inhibited monkey and human acetylcholinesterases. Chem. Biol. Interact. 175 (2008) 261-266
13. MacIlwain C. Study proves Iraq used nerve gas. Nature 363 (1993) 3
14. 2-terminal and active center sequences. J. Biol. Chem. 260 (1985) 12185-12189
15. Marrs T.C. Toxicology of organophosphate nerve agents. In: Marrs T.C., Maynard R.L., and Sidell F.R. (Eds). Chemical Warfare Agents: Toxicology and Treatment (2007), John Wiley & Sons Ltd., Chichester 191-221
16. Nozaki H., Aikawa N., Fujishima S., Suzuki M., Shinozawa Y., Hori S., and Nogawa S. A case of VX poisoning and the difference from sarin. Lancet 346 (1995) 698-699
17. Okumura T., Takasu N., Ishimatsu S., Miyanoki S., Mitsuhashi A., Kumuda K., Tanaka K., and Hinohara S. Report of 640 victims of the Tokyo subway sarin attack. Ann. Emerg. Med. 28 (1996) 129-135
18. Suzuki J., Kohno T., Tsukagosi M., Furuhata T., and Yamazaki K. Eighteen cases exposed to sarin in Matsumoto, Japan. Intern. Med. 36 (1997) 466-470
19. Taylor P., Radic Z., Hosca N.A., Camp S., Marchot P., and Berman H.A. Structural basis for the specificity of cholinesterase catalysis and inhibition. Toxicol. Lett. 82-83 (1995) 453-458
20. Worek F., Mast U., Kiderlen D., Diepold C., and Eyer P. Improved determination of acetylcholinesterase activity in human whole blood. Clin. Chim. Acta 288 (1999) 73-90
21. Worek F., Reiter G., Eyer P., and Szinicz L. Reactivation kinetics of acetylcholinesterase from different species inhibited by highly toxic organophosphates. Arch. Toxicol. 76 (2002) 523-529
22. Worek F., Aurbek N., Wetherell J., Pearce P., Mann T., and Thiermann H. Inhibition, reactivation and aging kinetics of highly toxic organophosphorus compounds: pig versus minipig acetylcholinesterase. Toxicology 244 (2008) 35-41