A single residue mutation abolishes attachment of the CBM26 starch-binding domain from Lactobacillus amylovorus α-amylase

Journal of Industrial Microbiology and Biotechnology

Volumn 36, Issue 3, 2009, Pages 341-346

  Rodríguez Sanoja, Romina ^a   Oviedo, N ^a   Escalante, L ^a   Ruiz, B ^a   Sanchez S ^a  

^a UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

Author keywords

Alpha amylase; Carbohydrate binding; CBM26; Starch; Starch binding domain

Indexed keywords

ALPHA-AMYLASES; AMINO ACID SEQUENCE; LACTOBACILLUS ACIDOPHILUS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN BINDING; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; STARCH; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 61349124174     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-008-0502-y     Document Type: Article

References (19)

1. RW Blakesly JA Boezi 1977 A new staining technique for protein in polyacrylamide gels using Coomassie brilliant blue G-250 Anal Biochem 82 580 582
2. AB Boraston DN Bolam HJ Gilbert GJ Davies 2004 Carbohydrate-binding modules: fine-tuning polysaccharide recognition Biochem J 382 769 781
3. AB Boraston M Healey J Klassen E Ficko-Blean BA van Lammerts VA Law 2006 Structural and functional analysis of alpha-glucan recognition by family 25 and 26 carbohydrate-binding modules reveals a conserved mode of starch recognition J Biol Chem 281 587 598
4. C Combet C Blanchet C Geourjon G Deléage 2000 NPS@: network protein sequence analysis Trends Biochem Sci 25 147 150
5. T Giardina AP Gunning N Juge CB Faulds CSM Furniss B Svensson VJ Morris G Williamson 2001 Both binding sites of the starch-binding domain of Aspergillus niger glucoamylase are essential for inducing a conformational change in amylose J Mol Biol 313 1149 1159
6. E Giraud G Cuny 1997 Molecular characterization of the alpha-amylase genes of Lactobacillus plantarum A6 and Lactobacillus amylovorus reveals an unusual 3 end structure with direct tandem repeats and suggests a common evolutionary origin Gene 198 149 157
7. González S (2006) Funciones adicionales del dominio de fijación al almidón de la α-amilasa de Lactobacillus amylovorus. Tesis Facultad de Química, UNAM
8. D Guillén M Santiago L Linares R Pérez J Morlon S Sánchez R Rodríguez-Sanoja 2007 Alpha-amylase starch binding domains: cooperative effects of binding to starch granules of multiple tandemly arranged domains Appl Environ Microbiol 73 3833 3837
9. UK Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685
10. BA van Lammerts AB Boraston 2007 The structural basis of α-glucan recognition by a family 41 carbohydrate-binding module from Thermotoga maritime J Mol Biol 365 555 560
11. HF Lo LL Lin WY Chiang MC Chi WH Hsu CT Chang 2002 Deletion analysis of the C-terminal region of the α-amylase of Bacillus sp. strain TS-23 Arch Microbiol 178 115 123
12. J Morlon-Guyot F Mucciolo-Roux R Rodríguez-Sanoja JP Guyot 2001 Characterization of the L. manihotivorans alpha-amylase gene DNA Seq 12 27 37
13. D Penninga VA van der Veen RMA Knegtel SAFT van Hijum H Rozeboom JKH Kalk BW Dijkstra L Dijkhuizen 1996 The raw starch binding domain of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 J Biol Chem 271 32777 32784
14. R Rodríguez-Sanoja J Morlon-Guyot J Jore J Pintado N Juge JP Guyot 2000 Comparative characterization of complete and truncated forms of Lactobacillus amylovorus alpha-amylase and role of the C-terminal direct repeats in raw-starch binding Appl Environ Microbiol 66 3350 3356
15. R Rodríguez-Sanoja N Oviedo S Sanchez 2005 Microbial starch-binding domain Curr Opin Microbiol 8 260 267
16. M Santiago L Linares S Sánchez R Rodríguez-Sanoja 2005 Functional characteristics of the starch-binding domain of Lactobacillus amylovorus α-amylase Biologia (Bratislava) 60 Suppl 16 111 114
17. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor
18. SM Southall PJ Simpson HJ Gilbert G Williamson MP Williamson 1999 The starch binding domain from glucoamylase disrupts the structure of starch FEBS Lett 447 58 60
19. J Sumitani T Tottori T Kawaguchi M Arai 2000 New type of starch binding domain: the direct repeat motif in the C-terminal region of Bacillus sp. no. 195 α-amylase contributes to starch binding and raw starch degrading Biochem J 350 477 484