Production and characterization of a panel of monoclonal antibodies against native human cellular prion protein

Hybridoma

Volumn 28, Issue 1, 2009, Pages 13-20

  Jones, Michael ^a   McLoughlin, Victoria ^b   Connolly, John G ^b   Farquhar, Christine F ^c   MacGregor, Ian R ^d   Head, Mark W ^a  

^a WESTERN GENERAL HOSPITAL   (United Kingdom)

^b UNIVERSITY OF STRATHCLYDE   (United Kingdom)

^c UNIVERSITY OF EDINBURGH   (United Kingdom)

^d SCOTTISH NATIONAL BLOOD TRANSFUSION SERVICE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; EPITOPE; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; PRION PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ANTIBODY RESPONSE; ARTICLE; CELL CLONE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; HUMAN TISSUE; HYBRID CELL; IMMUNIZATION; IMMUNOPRECIPITATION; MOUSE; MYELOMA CELL; NONHUMAN; PRIORITY JOURNAL; SPLEEN CELL; THROMBOCYTE;

ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; CREUTZFELDT-JAKOB SYNDROME; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; HUMANS; HYBRIDOMAS; IMMUNOPRECIPITATION; MICE; MICE, KNOCKOUT; PRPC PROTEINS; PRPSC PROTEINS;

MUS;

EID: 60849104835     PISSN: 15540014     EISSN: None     Source Type: Journal    
DOI: 10.1089/hyb.2008.0067     Document Type: Article

References (35)

1. Prusiner SB. Prions. Brain Pathol 1998;8:499-513.
2. Brown P, Brandel JP, Preece M, and Sato T: Iatrogenic Creutzfeldt-Jakob disease: the waning of an era. Neurology 2006;67:389-393.
3. Gajdusek DC, and Zigas V: Degenerative disease of the central nervous system in New Guinea. The endemic occurrence of "kuru" in the native population. N Engl J Med 1957;257:974-978.
4. Will RG, Ironside JW, Zeidler M, Cousens SN, Estibeiro K, Alperovitch A, Poser S, Pocchiari M, Hofman A, and Smith PG: A new variant of Creutzfeldt-Jakob disease in the UK. Lancet 1996;347:921-925.
5. Bruce ME, Will RG, Ironside JW, McConnell I, Drummond D, Suttie A, McCardle l, Chree A, Hope J, Birkett C, Cousens S, Fraser H, and Bostock CJ: Transmissions to mice indicate that "new variant" CJD is caused by the BSE agent. Nature 1997;389:498-501.
6. Stahl N, Borchelt DR and, Prusiner SB: Differential release of cellular and scrapie prion proteins from cellular membranes by phosphatidylinositol- specific phospholipase. Biochemistry 1990;29:5405-5412.
7. Prusiner SB: Molecular biology of prion diseases. Science 1991;252:1515-1522.
8. Prusiner SB. Prions. Proc Natl Acad Sci USA 1998;95:13363-13383.
9. Morel N, Simon S, Frobett Y, Volland H, Mourton-Gilles C, Negro A, Sorgato MC, Creminon C, and Grassi J: Selective and efficient immunoprecipitation of the disease-associated form of the prion protein can be mediated by non-specific interactions between monoclonal antibodies and scrapie-associated fibrils. J Biol Chem 2004;29:30143-30149.
10. Parchi P, Capellari S, Chen SG, Petersen RB, Gambetti P, Kopp N, Brown P, Kitamoto T, Tatieshi J, Giese A, and Kretzschamr H: Typing prion isoforms. Nature 1997;386:232-234.
11. Parchi P, Chen SG, Brown P, Zou W, Capellari S, Budka H, Hainfellner J, Reyes PF, Golden GT, Hauw JJ, Gajdusek CD, and Gambetti P: Different patterns of truncated prion protein fragments correlate with distinct phenotypes in P102L Gerstmann-Straussler-Scheinker disease. Proc Natl Acad Sci USA 1998;95:8322-8327.
12. Head MW, Bunn TP, Bishop MT, McLoughlin V, Lowrie S, McKimmie CS, Williams MC, McCardle L, MacKenzie J, Knight R, Will RG, and Ironside JW: Prion protein heterogeneity in sporadic but not variant Creutzfeldt-Jakob disease: UK cases 1991-2002. Ann Neurol 2004;55:851-859.
13. Yakovleva O, Janiak A, McKenzie C, McShane L, Brown P, and Cervenakova L: Effect of protease treatment on plasma infectivity in variant Creutzfeldt-Jakob disease. Transfusion 2004;44:1700-1705.
14. Safar J, Geschwind MD, Deering C, Didorenko S, Sattavat M, Sanchez H, Serban A, Vey M, Baron H, Giles K, Miller BL, DeArmond SJ, and Prusiner SB: Diagnosis of human prion disease. Proc Natl Acad Sci USA 2005;102:3501-3506.
15. Sc fraction. Biochemistry 2006;45:15710-15717.
16. Thackray AM, Hopkins L, and Bujdoso R: Proteinase K-sensitive disease- associated ovine prion protein revealed by conformation-dependent immunoassay. Biochem J 2007;401:475-483.
17. Kascsak RJ, Rubenstein R, Merz PA, Tonna-DeMasi M, Fersko R, Carp RI, Wisniewski HM, and Diringer H: Mouse polyclonal and monoclonal antibody to scrapie-associated fibril protein. J Virol 1987;61:3688-3693.
18. Sc)-specific epitope defined by a monoclonal antibody. Nature 1997;390:74-77.
19. Sc. Biochim Biophys Acta 2007;1774:1438-1450.
20. Sc of transmissible spongiform encephalopathies using specific monoclonal antibodies to PrP. Immunol Cell Biol 2005;83:632-637.
21. Vranac T, Hartman KP, Popovic M, Venturini A, Zerovnik E, and Serbec VC: A single prion protein peptide can elicit a panel of isoform specific monoclonal antibodies. Peptides 2006;27:2695-2705.
22. Jones M, Head MW, Connolly JG, Farquhar CF, Hornsey VS, Pepper DS, and MacGregor IR: Purification of normal cellular prion protein from human platelets and the formation of a high molecular weight prion protein complex following platelet activation. Biochem Biophys Res Commun 2005;335:48-56.
23. Manson JC, Clarke AR, Hoper ML, Aitchison L, McConnell I, and Hope J: 129/Ola mice carrying a null mutation in PrP that abolishes messenger-RNA production are developmentally normal. Mol Neurobiol 1994;8:121-127.
24. Harlow E, and Lane D. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1988.
25. Sc) from human brain specimens. Brain Path 2008:doi:10.111=j. 1750-3639.2008.00181.x
26. van Rheede T, Smolenaars MM, Madsen O, and de Jong WW: Molecular evolution of the mammalian prion protein. Mol Biol Evol 2003;20:111-121.
27. Prusiner SB, Groth D, Serban A, Koehler R, Foster D, Torchoa M, Burton D, Yang SL, and DeArmond SJ: Ablation of the prion protein (PrP) gene in mice prevents scrapie and facilitates production of anti-PrP antibodies. Proc Natl Acad Sci USA 1993;90:10608-10612.
28. Williamson RA, Peretz D, Smorodinsky N, Bastidas R, Serban H, Mehlhorn I, DeArmond SJ, Prusiner SB, and Burton DR: Circumventing tolerance to generate autologous monoclonal antibodies to the prion protein. Proc Natl Acad Sci USA 1996;93:7279-7282.
29. Rubenstein R, Kascsak RJ, Papini M, Kascsak R, Carp RI, LaFauci G, Meloen R, and Langeveld J: Immune surveillance and antigen conformation determines humoral immune response to the prion protein immunogen. J Neurovirol 1999;5:401-413.
30. Andrievskaia O, McRae H, Elmgren C, Huang H, Balachandran A, and Nilsen K: Generation of antibodies against bovine recombinant prion protein in various strains of mice. Clin Vaccine Immunol 2006;13:98-105.
31. Kim JI, Kuizon S, and Rubenstein R: Comparison of PrP transcription and translation in two murine hybridoma cell lines. J Neuroimmunol 2003;140:137-141.
32. Sellrie F, Schenk JA, Behrsing O, Drechsel O, and Micheel B: Cloning and characterization of a single chain antibody to glucose oxidase from a murine hybridoma. J Biochem Mol Biol 2007;40:875-880.
33. Paramithiotis E, Pinard M, Lawton T, LaBoissiere S, Leathers VL, Zou WQ, Estay LA, Lamontagne J, Lehto MT, Kondejewski LH, Francoeur GP, Papadopoulos M, Haghighat A, Spatz SJ, Head M, Will R, Ironside J, O'Rourke K, Tonelli Q, Ledebur HC, Chakrabartty A, and Cashman NR: A prion protein epitope selective for the pathologically misfolded conformation. Nature Medicine 2003;9:893-899.
34. Serbec VC, Bresjanac M, Popovic M, Hartman KP, Galvani V, Rupreht R, Cernilec M, Vranac T, Hafner I, and Jerala R: Monoclonal antibody against a peptide of human prion protein discriminates between Creutzeldt-Jakob's disease-affected and normal brain tissue. J Biol Chem 2004;279:3694-3698.
35. Bodemer W: The use of monoclonal antibodies in human prion disease. Naturwissenschaften 1999;86:z212-220.