Role of protease-activated receptor-1 in endothelial nitric oxide synthase-Thr495 Phosphorylation

Experimental Biology and Medicine

Volumn 234, Issue 2, 2009, Pages 132-139

  Watts, Vabren L ^a   Motley, Evangeline D ^a  

^a MEHARRY MEDICAL COLLEGE   (United States)

Author keywords

Endothelial NO synthase; Nitric oxide; Protease activated receptor; Rho; ROCK; Signaling; Thrombin

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; CALCIUM; CALPAIN; ENDOTHELIAL NITRIC OXIDE SYNTHASE; ENZYME INHIBITOR; EXOENZYME C3; G PROTEIN COUPLED RECEPTOR; NITRIC OXIDE; NITRITE; PEPTIDE; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE C DELTA; PROTEINASE ACTIVATED RECEPTOR 1; PROTEINASE ACTIVATED RECEPTOR 2; RHO KINASE INHIBITOR; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; SCH 79797; SERINE; THREONINE; THREONYLPHENYLALANYLLEUCYLLEUCYLARGININE; THROMBIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENDOTHELIAL DYSFUNCTION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME REGULATION; HUMAN; HUMAN CELL; IMMUNOBLOTTING; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

ADP RIBOSE TRANSFERASES; AMIDES; BOTULINUM TOXINS; CELLS, CULTURED; ENDOTHELIAL CELLS; GENES, DOMINANT; HUMANS; MODELS, BIOLOGICAL; NITRIC OXIDE SYNTHASE TYPE III; NITRITES; OLIGOPEPTIDES; PHOSPHORYLATION; PHOSPHOTHREONINE; PYRIDINES; PYRROLES; QUINAZOLINES; RECEPTOR, PAR-1; RECEPTOR, PAR-2; RHO-ASSOCIATED KINASES; RHOA GTP-BINDING PROTEIN; THROMBIN; UMBILICAL VEINS;

EID: 60549107030     PISSN: 15353702     EISSN: 15353699     Source Type: Journal    
DOI: 10.3181/0807-RM-233     Document Type: Article

References (17)

1. Hirano K. The roles of proteinase-activated receptors in the vascular physiology and pathophysiology. Arterioscler Thromb Vasc Biol 27: 27-36, 2007.
2. Macfarlane SR, Seatter MJ, Kanke T, Hunter GD, Plevin R. Proteinase-activated receptors. Pharmacol Rev 53:245-282, 2001.
3. O'Brien PJ, Prevost N, Molino M, Holinger MK, Woolkalis MJ, Woulfe DS, Brass LF. Thrombin responses in human endothelial cells. Contributions from receptors other than PARI include the trans-activation of PAR2 by thrombin-cleaved PAR1. J Biol Chem 275: 13502-13509, 2000.
4. Minami T, Sugiyama A, Wu SQ, Abid R, Kodama T, Aird WC. Thrombin and phenotypic modulation of the endothelium. Arterioscler Thromb Vasc Biol 24:41-53, 2004.
5. Fulton D, Gratton JP, Sessa WC. Post-translational control of endothelial nitric oxide synthase: why isn't calcium/calmodulin enough? J Pharmacol Exp Ther 299:818-824, 2001.
6. Long C, Cook LG, Hamilton SL, Wu G, Mitchell BM. FK506 binding protein 12/12.6 depletion increases endothelial nitric oxide synthase threonine 495 phosphorylation and blood pressure. Hypertension 49: 569-576, 2007.
7. Motley ED, Eguchi K, Patterson M, Palmer P, Suzuki H, Eguchi S. Mechanism of endothelial nitric oxide synthase phosphorylation and activation by Thrombin. Hypertension 49:429-431, 2007.
8. Bayless KJ, Davis GE. The Cdc42 and Rac1 GTPases are required for capillary lumen formation in three-dimensional extracellular matrices. J Cell Sci 115:1123-1136, 2002.
9. Palmer RM, Ferrige AG, Moncada S. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature 327:524-526, 1987.
10. Fleming I, Fisslthaler B, Dimmeler S, Kemp BE, Busse R. Phosphorylation of Thr495 regulates Ca2+/Calmodulin-dependent endothelial nitric oxide synthase activity. Circ Res 88:E68-E75, 2001.
11. Korkmaz Y, Bloch W, Steinritz D, Baumann MA, Addicks K, Schneider K, Raab WH. Bradykinin mediates phosphorylation of eNOS in odontoblasts. J Dent Res 85:536-541, 2006.
12. Eto M, Barandiér C, Rathgeb L, Kozai T, Joch H, Yang Z, Lüscher TF. Thrombin suppresses endothelial nitric oxide synthase and upregulates endothelin-converting enzyme-1 expression by distinct pathways: role of Rho/ROCK and mitogen-activated protein kinase. Circ Res 89:583-590, 2001.
13. Ming XF, Viswambharan H, Barandier C, Ruffieux J, Kaibuchi K, Rusconi S, Yang Z. Rho GTPase/Rho kinase negatively regulates endothelial nitric oxide synthase phosphorylation through the inhibition of protein kinase B/Akt in human endothelial cells. Mol Cell Biol 22: 8467-8477, 2002.
14. Sugimoto M, Nakayama M, Goto TM, Amano M, Komori K, Kaibuchi K. Rho-kinase phosphorylates eNOS at threonine 495 in endothelial cells. Biochem Biophys Res Commun 361:462-467, 2007.
15. Steinberg SF. The cardiovascular actions of protease-activated receptors. Mol Pharmacol 67:2-11, 2004.
16. Trebicka J, Hennenberg M, Laleman W, Shelest N, Biecker E, Schepke M, Nevens F, Sauerbruch T, Heller J. Atorvastatin lowers portal pressure in cirrhotic rats by inhibition of RhoA/Rho-kinase and activation of endothelial nitric oxide synthase. Hepatology 46:242-253, 2007.
17. Bernier SG, Haldar S, Michel T. Bradykinin-regulated interactions of the mitogen-activated protein kinase pathway with the endothelial nitric-oxide synthase. J Biol Chem 275:30707-30715, 2000.