Purification and characterization of Chromobacterium sp. DS-1 cholesterol oxidase with thermal, organic solvent, and detergent tolerance

Applied Microbiology and Biotechnology

Volumn 80, Issue 1, 2008, Pages 59-70

  Doukyu, Noriyuki ^a   Shibata, Kanpei ^a   Ogino, Hiroyasu ^b   Sagermann, Martin ^c  

^a TOYO UNIVERSITY   (Japan)

^b Osaka Prefecture University   (Japan)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Cholesterol oxidase; Chromobacterium; Detergent; Organic solvent; Protein purification; Thermal stability

Indexed keywords

ADSORPTION; DIELECTRIC RELAXATION; ENZYMES; FOOD ADDITIVES; KETONES; ORGANIC COMPOUNDS; ORGANIC SOLVENTS; PURIFICATION;

ADSORPTION SPECTRUM; CHOLESTEROL OXIDASE; CHROMOBACTERIUM; CULTURE SUPERNATANT; DETERGENT; EXTRACELLULAR; ORGANIC SOLVENT; PROTEIN PURIFICATION; PURIFIED ENZYMES; SCREENING METHODS; THERMAL STABILITY;

CHOLESTEROL;

3(OR 17)BETA HYDROXYSTEROID DEHYDROGENASE; CHOLESTANOL; CHOLESTEROL OXIDASE; DETERGENT; FLAVOPROTEIN; HYDROGEN PEROXIDE; ORGANIC SOLVENT; OXYGEN; PREGNENOLONE;

ADSORPTION; BACTERIUM; DETERGENT; ENZYME ACTIVITY; IDENTIFICATION METHOD; OXYGEN; PH; PROTEIN; STEROL;

ADSORPTION; ALKALINITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CHROMOBACTERIUM; ENZYME PURIFICATION; HEAT TOLERANCE; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; OXYGEN CONSUMPTION; PH MEASUREMENT; SCREENING; SPECTROSCOPY; SUPERNATANT; THERMOSTABILITY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CHOLESTEROL; CHOLESTEROL OXIDASE; CHROMOBACTERIUM; DRUG COMBINATIONS; ENZYME STABILITY; HEAT; KINETICS; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PEROXIDES; PHYLOGENY; SOLVENTS; SUBSTRATE SPECIFICITY; UREA;

CHROMOBACTERIUM; CHROMOBACTERIUM SP;

EID: 47149083247     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1526-y     Document Type: Article

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