Crystallization and preliminary X-Ray analysis of cholesterol oxidase from Brevibacterium sterolicum containing covalently bound FAD

Journal of Structural Biology

Volumn 116, Issue 2, 1996, Pages 317-319

  Croteau, Nathalie ^a   Vrielink, Alice ^a  

^a MCGILL UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLESTEROL OXIDASE; FLAVINE ADENINE NUCLEOTIDE;

ARTICLE; BREVIBACTERIUM; CRYSTALLIZATION; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL; X RAY ANALYSIS;

BREVIBACTERIUM; BREVIBACTERIUM STEROLICUM;

EID: 0029974599     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsbi.1996.0047     Document Type: Article

References (13)

1. Cavener, D. R. (1992) GMC oxidoreductases. A newly defined family of homologous proteins with diverse catalytic activities, J. Mol. Biol. 223, 811-814.
2. Collaborative Computational Project Number 4. (1994) The CCP4 suite: Programs for protein crystallography, Acta Crystallogr. D50, 760-763.
3. Crowther, R. A. (1972) The fast rotation function, in Rossmann, M. G. (Ed.), The Molecular Replacement Method, pp. 173-178, Gordon & Breach, New York.
4. Fukuda, H., Kawakatni, Y., and Nakamura, S. (1973) A method to screen anticholesterol substances produced by microbes and a new cholesterol oxidase produced by Streptomyces violascens, Chem. Pharm. Bull. 21, 2057-2060.
5. Jancarik, J., and Kim, S.-H. (1991) Sparse matrix sampling: a screening method for crystallization of proteins, J. Appl. Crystallogr. 24, 409-411.
6. Hecht, H. J., Kalisz, H. M., Hendle, J., Schmid, R. D., and Schomburg, D. (1993) Crystal structure of glucose oxidase from Aspergillus niger refined at 2.3 Å resolution, J. Mol. Biol. 229, 153-172.
7. Kaas, I. J., and Sampson, N. S. (1995) The isomerization catalyzed by Brevibacterium sterolicum cholesterol oxidase proceeds stereospecifically with one base, Biochem. Biophys. Res. Commun. 206, 688-693.
8. Li, J., Vrielink, A., Brick, P., and Blow, D. M. (1993) Crystal structure of cholesterol oxidase complexed with a steroid substrate: Implications for flavin adenine dinucleotide dependent alcohol oxidases, Biochemistry 32, 11507-11515.
9. Matthews, B. W. (1968) Solvent content of protein crystals, J. Mol. Biol. 33, 491-497.
10. McPherson, A. (1982) in Preparation and Analysis of Protein Crystals, Wiley, New York.
11. Otwinowski, Z. (1993) Oscillation Data Reduction Program, in Sawyer, L., Isaacs, N., and Bailey, S. (Eds.), Proceedings of the CCP4 Study Weekend: "Data Collection and Processing," January 29-30 1993, pp. 56-62, SERC Daresbury Laboratory, England.
12. Stadtman, T. C., Cherkes, A., and Anfinsen, C. B. (1954) Studies on the microbiological degradation of cholesterol, J. Biol. Chem. 206, 511-523.
13. Vrielink, A., Lloyd, L. F., and Blow, D. M. (1991) Crystal structure of cholesterol oxidase from Brevibacterium sterolicum refined at 1.8 Å resolution, J. Mol. Biol. 219, 533-554.