The NtcA-dependent P1 promoter is utilized for glnA expression in N2-fixing heterocysts of Anabaena sp. strain PCC 7120

Journal of Bacteriology

Volumn 186, Issue 21, 2004, Pages 7337-7343

  Valladares, Ana ^a   Muro Pastor, Alicia M ^a   Herrero, Antonia ^a   Flores, Enrique ^a,b  

^a UNIVERSITY OF SEVILLE   (Spain)

^b Universidad de Sevilla CSIC   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL RNA; GLUTAMATE AMMONIA LIGASE; NITROGEN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR NTCA; UNCLASSIFIED DRUG; AMMONIA; DNA BINDING PROTEIN;

ANABAENA; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM CULTURE; BINDING SITE; CONTROL SYSTEM; CONTROLLED STUDY; CULTURE MEDIUM; FUSION GENE; GENE EXPRESSION REGULATION; GENE MUTATION; GENE STRUCTURE; GLNA GENE; NITROGEN FIXATION; NITROGEN METABOLISM; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN FUNCTION; REGULATORY MECHANISM; RNA ISOLATION; RNA SYNTHESIS; TRANSCRIPTION REGULATION; CHEMISTRY; GENETIC TRANSCRIPTION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

ANABAENA; ANABAENA SP.; BACTERIA (MICROORGANISMS); CYANOBACTERIA; NEGIBACTERIA;

AMMONIA; ANABAENA; BASE SEQUENCE; BINDING SITES; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GLUTAMATE-AMMONIA LIGASE; MOLECULAR SEQUENCE DATA; NITROGEN FIXATION; PROMOTER REGIONS (GENETICS); TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

EID: 6044252400     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.186.21.7337-7343.2004     Document Type: Article

References (53)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 2003. Current protocols in molecular biology. Greene/Wiley-Interscience, New York, N. Y.
2. Barne, K. A., J. A. Bown, S. J. W. Busby, and S. D. Minchin. 1997. Region 2.5 of the Escherichia coli RNA polymerase σ70 subunit is responsible for the recognition of the "extended -10" motif at promoters. EMBO J. 13:4034-4040.
3. Bergsland, K. J., and R. Haselkorn. 1991. Evolutionary relationships among eubacteria, cyanobacteria, and chloroplasts: evidence from the rpoC1 gene of Anabaena sp. strain PCC 7120. J. Bacteriol. 173:3446-3455.
4. Black, T. A., Y. Cai, Y., and C. P. Wolk. 1993. Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol. Microbiol. 9:77-84.
5. Brahamsha, B., and R. Haselkorn. 1991. Isolation and characterization of the gene encoding the principal sigma factor of the vegetative cell RNA polymerase from the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol. 173:2442-2450.
6. Buikema, W. J., and R. Haselkorn. 1991. Characterization of a gene controlling heterocyst differentiation in the cyanobacterium Anabaena 7120. Genes Dev. 5:321-330.
7. Busby, S., and R. H. Ebright. 1997. Transcription activation at class II CAP-dependent promoters. Mol. Microbiol. 23:853-859.
8. Cai, Y., and C. P. Wolk. 1990. Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences. J. Bacteriol. 172:3138-3145.
9. Curtis, S. E., and J. A. Martin. 1994. The transcription apparatus and the regulation of transcription initiation, p. 613-639. In D. A. Bryant (ed.), The molecular biology of cyanobacteria. Kluwer Academic Publishers, Dordrecht, The Netherlands.
10. deHaseth, P. L., M. L. Zupancic, and M. T. Record, Jr. 1998. RNA polymerase-promoter interactions: the comings and goings of RNA polymerase. J. Bacteriol. 180:3019-3025.
11. Elbai, J., Y. Cai, and C. P. Wolk. 1994. Conduction of pEC22, a plasmid coding for MR.EcoT22I, mediated by a resident Tn3-like transposon, Tn5396. J. Bacteriol. 176:5059-5067.
12. Elhai, J., and C. P. Wolk. 1988. A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers. Gene 68:119-138.
13. Elhai, J., and C. P. Wolk, 1990. Developmental regulation and spatial pattern of expression of the structural genes for nitrogenase in the cyanobacterium Anabaena. EMBO J. 9:3379-3388.
14. Fisher, R., R. Tuli, and R. Haselkorn. 1981. A cloned cyanobacterial gene for glutamine synthetase functions in Escherichia coli, but the enzyme is not adenylylated. Proc. Natl. Acad. Sci. USA 78:3393-3397.
15. Frías, J. E., E. Flores, and A. Herrero. 1994. Requirement of the regulatory protein NtcA for the expression of nitrogen assimilation and heterocyst development genes in the cyanobacterium Anabaena sp. strain PCC 7120. Mol. Microbiol. 14:823-832.
16. Frías, J. E., E. Flores, and A. Herrero. 2000. Activation of the Anabaena nir operon promoter requires both NtcA (CAP family) and NtcB (LysR family) transcription factors. Mol. Microbiol. 38:613-625.
17. Giovannoni, S. J., S. Turner, G. J, Olsen, S. Barns, D. J. Lane, and N. R. Pace. 1988. Evolutionary relationships among cyanobacteria and green chloroplasts. J. Bacteriol. 170:3584-3592.
18. Golden, J. W., L. L. Whorff, and D. R. Wiest. 1991. Independent regulation of nifHDK operon transcription and DNA rearrangement during heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120. J. Bacteriol. 173:7098-7105.
19. Herrero, A., A. M. Muro-Pastor, and E. Flores. 2001. Nitrogen control in cyanobacteria. J. Bacteriol. 183:411-425.
20. Kokotek, W., and W. Lotz. 1989. Construction of a lacZ-kanamycin- resistance cassette, useful for site-directed mutagenesis and as a promoter probe. Gene 84:467-471.
21. Lindell, D., E. Padan, and A. F. Post. 1998. Regulation of ntcA expression and nitrite uptake in the marine Synechococcus sp. strain WH 7803. J. Bacteriol. 180:1878-1886.
22. Luque, I., E. Flores, and A. Herrero. 1994. Molecular mechanism for the operation of nitrogen control in cyanobacteria. EMBO J. 13:2862-2869.
23. Mackinney, G. 1941. Absorption of light by chlorophyll solutions. J. Biol. Chem. 140:315-322.
24. Markwell, M. A. K., S. M. Hass, L. L. Bieber, and N. E. Tolbert. 1978. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem. 87:206-210.
25. Marsh, J. L., M. Erfle, and E. J. Wykes. 1984. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481-485.
26. Mérida, A., L. Leurentop, P. Candau, and F. J. Florencio. 1990. Purification and properties of glutamine synthetases from the cyanobacteria Synechocystis sp. strain PCC 6803 and Calothrix sp. strain PCC 7601. J. Bacteriol. 172:4732-4735.
27. Merrick, M. J., and R. A. Edwards. 1995. Nitrogen control in bacteria. Microbiol. Rev. 59:604-622.
28. Montesinos, M. L., A. M. Muro-Pastor, A. Herrero, and E. Flores. 1998. Ammonium/methylammonium permeases of a cyanobacterium. Identification and analysis of three nitrogen-regulated amt genes in Synechocystis sp. PCC 6803. J. Biol. Chem. 273:31463-31470.
29. Mooney, R. A., I. Artsimovitch, and R. Landick. 1998. Information processing by RNA polymerase: recognition of regulatory signals during RNA chain elongation. J. Bacteriol. 180:3265-3275.
30. Mrázek, J., D. Bhaya, A. R. Grossman, and S. Karlin. 2001. Highly expressed and alien genes of the Synechocystis genome. Nucleic Acids Res. 29:1590-1601.
31. Muro-Pastor, A. M., E. Flores, A. Herrero, and C. P. Wolk. 1992. Identification, genetic analysis and characterization of a sugar-non-specific nuclease from the cyanobacterium Anabaena sp. strain PCC 7120. Mol. Microbiol. 6:3021-3030.
32. Muro-Pastor, A. M., T. Kuritz, E. Flores, A. Herrero, and C. P. Wolk. 1994. Transfer of a genetic marker from a megaplasmid of Anabaena sp. strain PCC 7120 to a megaplasmid of a different Anabaena strain. J. Bacteriol. 176:1093-1098.
33. Muro-Pastor, A. M., A. Valladares, E. Flores, and A. Herrero. 1999. The hetC gene is a direct target of the NtcA transcriptional regulator in cyanobacterial heterocyst development. J. Bacteriol. 181:6664-6669.
34. Muro-Pastor, A. M., A. Valladares, E. Flores, and A. Herrero. 2002. Mutual dependence of the expression of the cell differentiation regulatory protein HetR and the global nitrogen regulator NtcA during heterocyst development. Mol. Microbiol. 44:1377-1385.
35. Muro-Pastor, M. I., J. C. Reyes, and F. J. Florencio. 2001. Cyanobacteria perceive nitrogen status by sensing intracellular 2-oxoglutarate levels. J. Biol. Chem. 276:38320-38328.
36. Prentki, P., and H. M. Krisch. 1984. In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29:303-313.
37. Ramasubramanian, T. S., T.-F. Wei, and J. W. Golden. 1994. Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell-and heterocyst-specific genes. J. Bacteriol. 176:1214-1223.
38. Ramasubramanian, T. S., T.-F. Wei, A. K. Oldham, and J. W. Golden. 1996. Transcription of the Anabaena sp. strain PCC 7120 ntcA gene: multiple transcripts and NtcA binding. J. Bacteriol. 178:922-926.
39. Rippka, R., J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier. 1979. Generic assignments, strain stories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111:1-61.
40. Rojo, F. 1999. Repression of transcription initiation in bacteria. J. Bacteriol. 181:2987-2991.
41. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring harbor Laboratory, Cold Spring Harbor, N.Y.
42. Schneider, G. J., J. D. Lang, and R. Haselkorn. 1991. Promoter recognition by the RNA polymerase from vegetative cells of the cyanobacterium Anabaena 7120. Gene 105:51-60.
43. Schneider, G. J., N. E. Tumer, C. Richaud, G. Borbely, and R. Haselkorn. 1987. Purification and characterization of RNA polymerase from the cyanobacterium Anabaena 7120. J. Biol. Chem. 262:14633-14639.
44. Tanigawa, R., M. Shirokane, S. Maeda, T. Omata, K. Tanaka, and H. Takahashi. 2002. Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro. Proc. Natl. Acad. Sci. USA 99:4251-4255.
45. Turner, N. E., S. J. Robinson, and R. Haselkorn. 1983. Different promoters for the Anabaena glutamine synthetase gene during growth using molecular or fixed nitrogen. Nature 306:1-6.
46. Vázquez-Bermúdez, M. F., A. Herrero, and E. Flores. 2002. 2-Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter. FEBS Lett. 512:71-74.
47. Vázquez-Bermúdez, M. F., A. Herrero, and E. Flores. 2003. Carbon supply and 2-oxoglutarate effects on expression of nitrate reductase and nitrogen-regulated genes in Synechococcus sp. strain PCC 7942. FEMS Microbiol. Lett. 221:155-159.
48. Vázquez-Bermúdez, M. F., J. Paz-Yepes, A. Herrero, and E. Flores. 2002. The NtcA-activated amt1 gene encodes a permease required for uptake of low concentrations of ammonium in the cyanobacterium Synechococcus sp. PCC 7942. Microbiology 148:861-869.
49. Wei, T.-F., T. S. Ramasubramanian, and J. W. Golden. 1994. Anabaena sp. strain PCC 7120 ntcA gene required for growth on nitrate and heterocyst development. J. Bacteriol. 176:4473-4482.
50. Wolk, C. P., A Ernst, and J. Elhai. 1994. Heterocyst metabolism and development, p. 769-823. In D. A. Bryant (ed.), The molecular biology of cyanobacteria. Kluwer Academic Publishers, Dordrecht, The Netherlands.
51. 13N-labeled nitrogen gas by the cyanobacterium, Anabaena cylindrica. J. Biol. Chem. 251:5027-5034.
52. Wolk, C. P., A. Vonshak, P. Kehoe, and J. Elhai. 1984. Construction of shuttle vectors capable of conjugative transfer from Escherichia coli to nitrogen-fixing filamentous cyanobacteria. Proc. Natl. Acad. Sci. USA 81: 1561-1565.
53. Xie, W. Q., K. Jäger, and M. Potts. 1989. Cyanobacterial RNA polymerase genes rpoC1 and rpoC2 correspond to rpoC of Escherichia coli. J. Bacteriol. 171:1967-1973.