Carbon supply and 2-oxoglutarate effects on expression of nitrate reductase and nitrogen-regulated genes in Synechococcus sp. strain PCC 7942

FEMS Microbiology Letters

Volumn 221, Issue 2, 2003, Pages 155-159

  Vázquez Bermúdez, Maria Félix ^a   Herrero, Antonia ^a   Flores, Enrique ^a  

^a UNIVERSITY OF SEVILLE   (Spain)

Author keywords

Cyanobacteria; Nitrogen control; NtcA

Indexed keywords

2 OXOGLUTARIC ACID; BICARBONATE; CARBON DIOXIDE; MESSENGER RNA; NITRATE REDUCTASE; ALPHA-KETOGLUTARIC ACID; BACTERIAL PROTEIN; CARBON; DICARBOXYLATE TRANSPORTER; DNA BINDING PROTEIN; ESCHERICHIA COLI PROTEIN; KGTP PROTEIN, E COLI; NITROGEN; NTCA PROTEIN, SYNECHOCOCCUS; TRANSCRIPTION FACTOR;

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; CARBON NITROGEN RATIO; CARBON SOURCE; ENZYME ACTIVITY; GENE CONTROL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INDUCTION; PSBA1 GENE; SYNECHOCOCCUS; CYANOBACTERIUM; DRUG EFFECT; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; REGULATOR GENE;

CYANOBACTERIA; SYNECHOCOCCUS; SYNECHOCOCCUS SP.; SYNECHOCOCCUS SP. PCC 6301;

BACTERIAL PROTEINS; CARBON; CYANOBACTERIA; DICARBOXYLIC ACID TRANSPORTERS; DNA-BINDING PROTEINS; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REGULATOR; KETOGLUTARIC ACIDS; NITRATE REDUCTASE; NITRATE REDUCTASES; NITROGEN; TRANSCRIPTION FACTORS;

EID: 0142062422     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00208-8     Document Type: Article

References (32)

1. Merrick, M.J. and Edwards, R.A. (1995) Nitrogen control in bacteria. Microbiol. Rev. 59, 604-622.
2. Jiang, P., Peliska, J.A. and Ninfa, A.J. (1998) Reconstitution of the signal-transduction bicyclic cascade responsible for the regulation of Ntr gene transcription in Escherichia coli. Biochemistry 37, 12795-12801.
3. II signal transduction proteins, pivotal players in microbial nitrogen control. Microbiol. Mol. Biol. Rev. 65, 80-105.
4. Flores, E. and Herrero, A. (1994) Assimilatory nitrogen metabolism and its regulation, in: The Molecular Biology of Cyanobacteria (Bryant, D.A., Ed.), pp. 487-517. Kluwer Academic Publishers, Dordrecht.
5. Herrero, A., Muro-Pastor, A.M. and Flores, E. (2001) Nitrogen control in cyanobacteria. J. Bacteriol. 183, 411-425.
6. Vázquez-Bermúdez, M.F., Paz-Yepes, J., Herrero, A. and Flores, E. (2002) The NtcA-activated amt1 gene encodes a permease required for uptake of low concentrations of ammonium in the cyanobacterium Synechococcus sp. PCC 7942. Microbiology 148, 861-869.
7. Luque, I., Flores, E. and Herrero, A. (1993) Nitrite reductase gene from Synechococcus sp. PCC 7942: homology between cyanobacterial and higher-plant nitrite reductases. Plant Mol. Biol. 21, 1201-1205.
8. Omata, T. (1995) Structure, function and regulation of the nitrate transport system of the cyanobacterium Synechococcus sp. PCC7942. Plant Cell Physiol. 36, 207-213.
9. Rubio, L.M., Herrero, A. and Flores, E. (1996) A cyanobacterial narB gene encodes a ferredoxin-dependent nitrate reductase. Plant Mol. Biol. 30, 845-850.
10. Herrero, A., Flores, E. and Guerrero, M.G. (1981) Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp. strain 7119, and Nostoc sp. strain 6719. J. Bacteriol. 145, 175-180.
11. Smith, A.J., London, J. and Stainer, R.Y. (1967) Biochemical basis of obligate autotrophy in blue-green algae and thiobacilli. J. Bacteriol. 94, 972-983.
12. Vázquez-Bermúdez, M.F., Herrero, A. and Flores, E. (2000) Uptake of 2-oxoglutarate in Synechococcus strains transformed with the Escherichia coli kgtP gene. J. Bacteriol. 182, 211-215.
13. Muro-Pastor, M.I., Reyes, J.C. and Florencio, F.J. (2001) Cyanobacteria perceive nitrogen status by sensing intracellular 2-oxoglutarate levels. J. Biol. Chem. 276, 38320-38328.
14. Vázquez-Bermúdez, M.F., Herrero, A. and Flores, E. (2002) 2-Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus gin A promoter. FEBS Lett. 512, 71-74.
15. Tanigawa, R., Shirokane, M., Maeda, S.-I., Omata, T., Tanaka, K. and Takahashi, H. (2002) Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro. Proc. Natl. Acad. Sci. USA 99, 4251-4255.
16. Rippka, R., Deruelles, J., Waterbury, J.B., Herdman, M. and Stanier, R.Y. (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J. Gen. Microbiol. 111, 1-61.
17. Mackinney, G. (1941) Absorption of light by chlorophyll solutions. J. Biol. Chem. 140, 315-322.
18. Herrero, A., Flores, E. and Guerrero, M.G. (1985) Regulation of nitrate reductase cellular levels in the cyanobacteria Anabaena variabilis and Synechocystis sp., FEMS Microbiol. Lett. 26, 21-25.
19. Shapiro, B.M. and Stadtman, E.R. (1970) Glutamine synthetase (E. coli). Methods Enzymol. 17A, 910-922.
20. Luque, I., Flores, E. and Herrero, A. (1994) Molecular mechanism for the operation of nitrogen control in cyanobacteria. EMBO J. 13, 2862-2869.
21. Ausubel, P.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K. (2001) Currents Protocols in Molecular Biology. Greene Publishing and Wiley Interscience, NY.
22. Banta, A.B., Haas, E.S., Brown, J.W. and Pace, N.R. (1992) Sequence of the ribonuclease P RNA gene from the cyanobacterium Anacystis nidulans. Nucleic Acids Res. 20, 911.
23. 2 limitation in the cyanobacterium Synechococcus sp. PCC7942. Plant Physiol. 107, 791-796.
24. Montesinos, M.L., Herrero, A. and Flores, E. (1997) Amino acid transport in taxonomically diverse cyanobacteria and identification of two genes encoding elements of a neutral amino acid permease putatively involved in recapture of leaked hydrophobic amino acids. J. Bacteriol. 179, 853-862.
25. Golden, S.S., Brusslan, J.A. and Haselkorn, R. (1986) Expression of a family of psbA genes encoding a photosystem II polypeptide in the cyanobacterium Anacystis nidulans R2. EMBO J. 5, 2789-2798.
26. Suzuki, I., Sugiyama, T. and Omata, T. (1996) Regulation by cyanate of the genes involved in carbon and nitrogen assimilation in the cyanobacterium Synechococcus sp. strain PCC 7942. J. Bacteriol. 178, 2688-2694.
27. II (GlnB) and influences its phosphorylation level in response to nitrogen and carbon supplies in the cyanobacterium Synechococcus sp. strain PCC 7942. J. Bacteriol. 181, 2697-2702.
28. Romero, J.M., Lara, C. and Guerrero, M.G. (1985) Dependence of nitrate utilization upon active COi fixation in Anacystis nidulans: a regulatory aspect of the interaction between photosynthetic carbon and nitrogen metabolism. Arch. Biochim. Biophys. 237, 396-401.
29. II protein (glnB gene product) in the cyanobacterium Synechococcus sp. strain PCC 7942: analysis of in vitro kinase activity. J. Bacteriol. 177, 5812-5817.
30. II in Synechococcus PCC 7942: identification of an ATP and 2-oxoglutarate-regulated phosphatase activity. Mol. Microbiol. 26, 81-90.
31. II in the control of nitrate/nitrite uptake in a cyanobacterium. FEBS Lett. 427, 291-295.
32. II-mediated regulation of nitrate and nitrite uptake in the cyanobacterium Synechococcus sp. PCC 7942. Eur. J. Biochem. 267, 591-600.