Investigation of the pathway for inter-copper electron transfer in peptidylglycine α-amidating monooxygenase

Journal of the American Chemical Society

Volumn 126, Issue 41, 2004, Pages 13168-13169

  Francisco, Wilson A ^a,d   Wille, Georg ^a,e   Smith, Alan J ^b   Merkler, David J ^c   Klinman, Judith P ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b STANFORD UNIVERSITY MEDICAL CENTER   (United States)

^c UNIVERSITY OF SOUTH FLORIDA   (United States)

^d ARIZONA STATE UNIVERSITY   (United States)

^e MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA AMIDATING ENZYME; COPPER; DEUTERIUM; GLYCINE DERIVATIVE; N ACETYLDIIODOTYROSYLGLYCINE; PROTON; UNCLASSIFIED DRUG; WATER;

ARTICLE; CATALYSIS; ELECTRON TRANSPORT; ENZYME ACTIVE SITE; ENZYME KINETICS; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROPHOBICITY; HYDROXYLATION; REACTION ANALYSIS; STEREOCHEMISTRY;

COPPER; DOPAMINE BETA-HYDROXYLASE; KINETICS; MIXED FUNCTION OXYGENASES; MODELS, MOLECULAR; MULTIENZYME COMPLEXES; PROTEIN CONFORMATION; THERMODYNAMICS;

EID: 6044235540     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja046888z     Document Type: Article

References (18)

1. Stewart, L. C.; Klinman, J. P. Annu. Rev. Biochem. 1988, 57, 551-592.
2. Eipper, B. A.; Stoffers, D. A.; Mains, R. E. Annu. Rev. Neurosci. 1992, 15, 57-85.
3. Prigge, S. T.; Mains, R. E.; Eipper, B. A.; Amzel, L. M. Cell. Mol. Life Sci. 2000, 57, 1236-1259.
4. Prigge, S. T.; Kolhekar, A. S.; Eipper, B. A.: Mains, R. E.; Amzel, L. M. Science 1997, 278, 1300-1305.
5. Prigge, S. T.; Kolhekar, A. S.; Eipper, B. A.; Mains, R. E.; Amzel, L. M. Nat. Struct. Biol. 1999, 6, 976-983.
6. Brenner, M. C.; Klinman, J. P. Biochemistry 1989, 28, 4664-4670.
7. Freeman, J. C.; Villafranca, J. J.; Merkler, D. J. J. Am. Chem. Soc. 1993 115, 4923-4924.
8. Jaron, S.; Blackburn, N. J. Biochemistry 1999, 38, 15086-15096.
9. Bell, J.; Meskini, R. E.; D'Amato, D.; Mains, R. E.; Eipper, B. A. Biochemistry 2003 42, 7133-7142.
10. Evans, J. P.; Ahn, K.; Klinman, J. P. J. Biol. Chem. 2003, 278, 49691-49698.
11. 2]-glycine (Cambridge Isotope Laboratories, Inc., Andover, MA) and 9-fluorenylmethyl oxycarbonyl N-hydroxysuccinimide following the procedure in ref 12. The N-dansylated peptides were purified by semipreparative HPLC and analyzed for isotopic composition by mass spectrometry.
12. Lapatsanis, L.; Milias, G.; Froussios, K.; Kolovos, M. Synthesis 1983, 671-673.
13. 14 The extended peptide 2 shows a preferred ordered steady-state kinetic pathway in which the substrate binds first (this study).
14. Francisco, W. A.; Merkler, D. J.; Blackburn, N. J.; Klinman, J. P. Biochemistry 1998, 37, 8244-8252.
15. Ponce, A.; Gray, H. B.; Winkler, J. R. J. Am. Chem. Soc. 2000, 122, 8187-8191.
16. Owen, T. C.; Merkler, D. J. Med. Hypotheses 2004, 62, 392-400.
17. Chen, P.; Solomon, E. I. J. Am. Chem. Soc. 2004, 126, 4991-5000.
18. Supported by GM grants from the NIH to JPK (25765), DJM (67257) and WAF (17026). We thank J. Evans and C. Hess (UCB) and H. Gray (Cal Tech) for valuable input.