The use of FRET microscopy to elucidate steady state channel conformational rearrangements and G protein interaction with the GIRK channels

Methods in Molecular Biology

Volumn 491, Issue , 2008, Pages 199-212

  Raveh, Adi ^a   Riven, Inbal ^b   Reuveny, Eitan ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b TEL AVIV UNIVERSITY   (Israel)

Author keywords

Anisotropy measurements; Fluorescence spectroscopy; FRET; GIRK; GPCR; TIRF microcopy

Indexed keywords

EID: 60349118066     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-59745-526-8_16     Document Type: Article

References (25)

1. Lakowicz, J. R. (2006) Principles of fluorescence spectroscopy, 3rd Edition. Springer, Berlin.
2. Stryer, L. (1978) Fluorescence energy transfer as a spectroscopic ruler. Annu. Rev. Biochem. 47, 819-846.
3. Wallrabe, H. and Periasamy, A. (2005) Imaging protein molecules using FRET and FLIM microscopy. Curr. Opin. Biotechnol. 16, 19-27.
4. Nguyen, A. W. and Daugherty, P. S. (2005) Evolutionary optimization of fluorescent proteins for intracellular FRET. Nat. Biotechnol. 23, 355-360.
5. Jares-Erijman, E. A. and Jovin, T. M. (2003) FRET imaging. Nat. Biotechnol. 21, 1387-1395.
6. Miyawaki, A. and Tsien, R. Y. (2000) Monitoring protein conformations and interactions by fluorescence resonance energy transfer between mutants of green fluorescent protein. Methods Enzymol. 327, 472-500.
7. Bastiaens, P. I., Majoul, I. V., Verveer, P. J., Soling, H. D., and Jovin, T. M. (1996) Imaging the intracellular trafficking and state of the AB5 quaternary structure of cholera toxin. EMBO J. 15, 4246-4253.
8. Tsien, R. Y. (1998) The green fluorescent protein. Annu. Rev. Biochem. 67, 509-544.
9. Riven, I., Iwanir, S., and Reuveny, E. (2006) GIRK channel activation involves a local rearrangement of a preformed G protein channel complex. Neuron 51, 561-573.
10. Riven, I., Kalmanzon, E., Segev, L., and Reuveny, E. (2003) Conformational rearrangements associated with the gating of the G protein-coupled potassium channel revealed by FRET microscopy. Neuron 38, 225-235.
11. + channels (GIRKs) mediate postsynaptic but not presynaptic transmitter actions in hippocampal neurons. Neuron 19, 687-695.
12. Kuo, A., Gulbis, J. M., Antcliff, J. F., Rahman, T., Lowe, E. D., Zimmer, J., Cuthbertson, J., Ashcroft, F. M., Ezaki, T., and Doyle, D. A. (2003) Crystal structure of the potassium channel KirBac1.1 in the closed state. Science 300, 1922-1926.
13. Nishida, M. and MacKinnon, R. (2002) Structural basis of inward rectification, cytoplasmic pore of the G protein-gated inward rectifier GIRK1 at 1.8 A resolution. Cell 111, 957-965.
14. Pegan, S., Arrabit, C., Zhou, W., Kwiatkowski, W., Collins, A., Slesinger, P. A., and Choe, S. (2005) Cytoplasmic domain structures of Kir2.1 and Kir3.1 show sites for modulating gating and rectification. Nat. Neurosci. 8, 279-287.
15. + conduction and selectivity. Science 280, 69-77.
16. Jiang, Y., Lee, A., Chen, J., Cadene, M., Chait, B. T., and MacKinnon, R. (2002) Crystal structure and mechanism of a calcium-gated potassium channel. Nature 417, 515-522.
17. Nishida, M., Cadene, M., Chait, B. T., and MacKinnon, R. (2007) Crystal structure of a Kir3.1-prokaryotic Kir channel chimera. EMBO J. 26, 4005-4015.
18. + channels. Nat. Rev. Neurosci. 4, 957-967.
19. + channel, subunit stoichiometry and structural domains essential for G protein stimulation. Am. J. Physiol. 271, H379-H385.
20. + channel. J. Biol. Chem. 271, 30524-30528.
21. Sadja, R., Alagem, N., and Reuveny, E. (2002) Graded contribution of the Gbeta gamma binding domains to GIRK channel activation. Proc. Natl Acad. Sci. USA 99, 10783-10788.
22. Heim, R. and Tsien, R. Y. (1996) Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6, 178-182.
23. Axelrod, D. (1989). Total internal reflection fluorescence microscopy. Methods Cell Biol. 30, 245-270.
24. Axelrod, D., Thompson, N. L., and Burghardt, T. P. (1983) Total internal inflection fluorescent microscopy. J. Microsc. 129, 19-28.
25. Reuveny, E., Slesinger, P. A., Inglese, J., Morales, J. M., Iniguez-Lluhi, J. A., Lefkowitz, R. J., Bourne, H. R., Jan, Y. N., and Jan, L. Y. (1994) Activation of the cloned muscarinic potassium channel by G protein beta gamma subunits. Nature 370, 143-146