Cryoelectron microscopy of vitreous sections: A step further towards the native state

Methods in Molecular Biology

Volumn 464, Issue , 2008, Pages 425-439

  Bouchet Marquis, Cedric ^a   Fakan, Stanislav ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

Author keywords

Cell nucleus; CEMOVIS; Cryoelectron microscopy; Cryoelectron microscopy of vitreous sections; Cryofixation; Vitrified sections

Indexed keywords

EID: 60349116495     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-461-6_24     Document Type: Article

References (20)

1. Monneron, A. and Bernhard, W. (1969) Fine structural organization of the interphase nucleus in some. mammalian cells. J. Ultrastruct. Res. 27, 266-288.
2. Fakan, S. (2004) The functional architecture of the nucleus as analysed by ultrastructural cytochemistry. Histochem. Cell Biol. 122, 83-93.
3. Tokuyasu, K. T. (1973) A technique for ultracryotomy of cell suspensions and tissues. J. Cell Biol. 57, 551-565.
4. McDowall, A. W., Chang, J. J., Freeman, R., Lepault, J., Walter, C. A., and Dubochet, J. (1983) Electron microscopy of frozen hydrated sections of vitreous ice and vitrified biological samples. J. Microsc. 131, 1-9.
5. McDowall, A. W., Hofmann, W., Lepault, J., Adrian, M., and Dubochet, J. (1984) Cryoelectron microscopy of vitrified insect flight muscle. J. Mol. Biol. 178, 105-111.
6. McDowall, A. W., Smith, J. M., and Dubochet, J. (1986) Cryo-electron microscopy of vitrified chromosomes in situ. EMBO J. 5, 1395-1402.
7. Sartori Blanc, N., Senn, A., Leforestier, A., Livolant, F., and Dubochet, J. (2001) DNA in human and stallion spermatozoa forms local hexagonal packing with twist and many defects J. Struct. Biol. 134, 76-81.
8. Al-Amoudi, A., Chang, J. J., Leforestier, A., McDowall, A., Salamin, L. M., Norlen, L. P., Richter, K., Blanc, N. S., Studer, D., and Dubochet, J. (2004) Cryoelectron microscopy of vitreous sections. EMBO J. 23, 3583-3588.
9. Zuber, B., Nikonenko, I., Klauser, P., Muller, D., and Dubochet, J. (2005) The mammalian central nervous synaptic cleft contains a high density of periodically organized complexes. Proc. Natl. Acad. Sci. USA 102, 19192-19197.
10. Eltsov, M. and Dubochet, J. (2005) Fine structure of the Deinococcus radiodurans nucleoid revealed by cryoelectron microscopy of vitreous sections. J. Bacteriol. 187, 8047-8054.
11. Bouchet-Marquis, C., Dubochet, J., and Fakan, S. (2006) Cryoelectron microscopy of vitrified sections: a new challenge for the analysis of functional nuclear architecture. Histochem. Cell Biol. 125, 43-51.
12. Dubochet, J. and Sartori Blanc, N. (2001) The cell in absence of aggregation artifacts. Micron 32, 91-99.
13. Studer, D., Graber, W., Al-Amoudi, A., and Eggli, P. (2001) A new approach for cryofixation by high-pressure freezing. J. Microsc. 203, 285-294.
14. Eisenberg, D. and Kauzmann, W. (1969) The Structure and Properties of Water. Oxford University Press, Oxford.
15. Franks, F. (1982) Water: A comprehensive treatise. In: Water and aqueous solutions at subzero temperatures (Franks, F., Ed.), Plenum Press, New York, pp. 215-338.
16. Dubochet, J., Adrian, M., Chang, J. J., Homo, J. C., Lepault, J., McDowall, A. W., and Schultz, P. (1988) Cryo-electron microscopy of vitrified specimens. Q. Rev. Biophys. 21, 129-228.
17. Dubochet, J., Al-Amoudi, A., Bouchet-Marquis, C., Eltsov, M., and Zuber, B. (in press) CEMOVIS. Cryo-electron microscopy of vitreous sections. In: Modern Cryopreparation methods for electron microscopy (Cavalier, A., Humbel, B. M., and Spehner, A., eds.), CRC.
18. Al-Amoudi, A., Studer, D., and Dubochet, J. (2005) Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy. J. Struct. Biol. 150, 109-121.
19. Al-Amoudi, A., Dubochet, J., Gnaegi, H., Luthi, W., and Studer, D. (2003) An oscillating cryo-knife reduces cutting induced deformation of vitreous ultrathin sections. J. Microsc. 212, 26-33.
20. Sartori Blanc, N., Studer, D., Ruhl, K., and Dubochet, J. (1998) Electron beam-induced changes in vitreous sections of biological samples. J Microsc 192, 194-201.