In situ hybridization study of the distribution of choline acetyltransferase mRNA and its splice variants in the mouse brain and spinal cord

Neuroscience

Volumn 159, Issue 1, 2009, Pages 344-357

  Trifonov, S ^a   Houtani, T ^a   Hamada, S ^a   Kase, M ^a   Maruyama, M ^a   Sugimoto, T ^a  

^a KANSAI MEDICAL UNIVERSITY   (Japan)

Author keywords

5 noncoding region; choline acetyltransferase gene organization; cholinergic gene locus; cholinergic neuron; medial habenular nucleus; noncoding choline acetyltransferase exons

Indexed keywords

CHOLINE ACETYLTRANSFERASE; DIGOXIGENIN; ISOENZYME; MESSENGER RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; AUTONOMIC NERVOUS SYSTEM; BRAIN; BRAIN CELL; BRAIN CORTEX; BRAIN NERVE CELL; BRAIN NUCLEUS; BRAIN REGION; BRAIN STEM; CELLULAR DISTRIBUTION; CENTRAL NERVOUS SYSTEM; CHOLINERGIC SYSTEM; COMPARATIVE STUDY; CORPUS STRIATUM; ENZYME LOCALIZATION; FOREBRAIN; HABENULA; HIPPOCAMPUS; HISTOCHEMISTRY; IN SITU HYBRIDIZATION; MALE; MOTONEURON NUCLEUS; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA PROBE; SPINAL CORD; SPINAL CORD VENTRAL HORN; TEGMENTUM; TISSUE DISTRIBUTION;

ANIMALS; BRAIN; CHOLINE O-ACETYLTRANSFERASE; IN SITU HYBRIDIZATION; MALE; MICE; MICE, INBRED C57BL; PROTEIN ISOFORMS; RNA, MESSENGER; SPINAL CORD;

EID: 60349103335     PISSN: 03064522     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.neuroscience.2008.12.054     Document Type: Article

References (41)

1. Aid T., Kazantseva A., Piirsoo M., Palm K., and Timmusk T. Mouse and rat BDNF gene structure and expression revisited. J Neurosci Res 85 (2007) 525-535
2. Bejanin S., Cervini R., Mallet J., and Berrard S. A unique gene organization for two cholinergic markers, choline acetyltransferase and a putative vesicular transporter of acetylcholine. J Biol Chem 269 (1994) 21944-21947
3. Bellier J., and Kimura H. Acetylcholine synthesis by choline acetyltransferase of a peripheral type as demonstrated in adult rat dorsal root ganglion. J Neurochem 101 (2007) 1607-1618
4. Braissant O., and Wahli W. A simplified in situ hybridization protocol using non-radioactively labeled probes to detect abundant and rare mRNAs on tissue sections. Biochemica 1 (1998) 10-16
5. Butcher L., and Woolf N. Cholinergic neurons and networks revisited. In: Paxinos G. (Ed). The rat nervous system. 3rd ed (2004), Academic Press, San Diego 1257-1268
6. Eiden L.E. The cholinergic gene locus. J Neurochem 70 (1998) 2227-2240
7. Ferrante R.J., Beal M.F., Kawall N.W., Richardson Jr. E.P., and Martin J.B. Sparing of acetylcholinesterase-containing striatal neurons in Huntington's disease. Brain Res 411 (1987) 162-166
8. Hahm S., Chen L., Patel C., Erickson J., Bonner T.I., Weihe E., Schäfer M.K., and Eiden L.E. Upstream sequencing and functional characterization of the human cholinergic gene locus. J Mol Neurosci 9 (1997) 223-236
9. Härtig W., Bauer A., Brauer K., Grosche J., Hortobágyi T., Penke B., Schliebs R., and Harkany T. Functional recovery of cholinergic basal forebrain neurons under disease conditions: old problems, new solutions?. Rev Neurosci 13 (2002) 95-165
10. Houser C.R., Crawford G.D., Barber R.P., Salvaterra P.M., and Vaughn J.E. Organization and morphological characteristics of cholinergic neurons: an immunocytochemical study with a monoclonal antibody to choline acetyltransferase. Brain Res 266 (1983) 97-119
11. Ibanez C.F., Ernfors P., and Persson H. Developmental and regional expression of choline acetyltransferase mRNA in the rat central nervous system. J Neurosci Res 29 (1991) 163-171
12. Ichikawa T., and Hirata Y. Organization of choline acetyltransferase-containing structures in the forebrain of the rat. J Neurosci 6 (1986) 281-292
13. Ishii K., Oda Y., Ichikawa T., and Deguchi T. Complementary DNAs for choline acetyltransferase from spinal cords of rat and mouse: nucleotide sequences, expression in mammalian cells, and in situ hybridization. Brain Res Mol Brain Res 7 (1990) 151-159
14. Kása P., Rakonczay Z., and Gulya K. The cholinergic system in Alzheimer's disease. Prog Neurobiol 52 (1997) 511-535
15. Kasashima S., Muroishi Y., Futakuchi H., Nakanishi I., and Oda Y. In situ mRNA hybridization study of the distribution of choline acetyltransferase in the human brain. Brain Res 806 (1998) 8-15
16. Kasashima S., Kawashima A., Muroishi Y., Futakuchi H., Nakanishi I., and Oda Y. Neurons with choline acetyltransferase immunoreactivity and mRNA are present in the human cerebral cortex. Histochem Cell Biol 111 (1999) 197-207
17. Kato T. Choline acetyltransferase activities in single spinal motor neurons from patients with amyotrophic lateral sclerosis. J Neurochem 52 (1989) 636-640
18. Kawashima K., Yoshikawa K., Fujii Y., Moriwaki Y., and Misawa H. Expression and function of genes encoding cholinergic components in murine immune cells. Life Sci 80 (2007) 2314-2319
19. Kengaku M., Misawa H., and Deguchi T. Multiple mRNA species of choline acetyltransferase from rat spinal cord. Brain Res Mol Brain Res 18 (1993) 71-76
20. Lauterborn J.C., Isackson P.J., Montalvo R., and Gall C.M. In situ hybridization localization of choline acetyltransferase mRNA in adult rat brain and spinal cord. Brain Res Mol Brain Res 17 (1993) 59-69
21. Mesulam M., Mufson E., Wainer B., and Levey A. Central cholinergic pathways in the rat: an overview based on an alternative nomenclature (Ch 1-Ch 6). Neuroscience 10 (1983) 1185-1201
22. Mesulam M., Geula C., Bothwell M., and Hersh L. Human reticular formation: cholinergic neurons of the pedunculopontine and laterodorsal tegmental nuclei and some cytochemical comparisons to forebrain cholinergic neurons. J Comp Neurol 281 (1989) 611-633
23. Misawa H., Ishii K., and Deguchi T. Gene expression of mouse choline acetyltransferase. Alternative splicing and identification of a highly active promoter region. J Biol Chem 267 (1992) 20392-20399
24. Oda Y., Yamashita N., Muroishi Y., and Nakanishi I. Localization of choline acetyltransferase and acetylcholine in the chorion of early human pregnancy. Histochem Cell Biol 105 (1996) 93-99
25. Oda Y. Choline acetyltransferase: the structure, distribution and pathologic changes in the central nervous system. Pathol Int 49 (1999) 921-937
26. Oda Y., and Nakanishi I. The distribution of cholinergic neurons in the human central nervous system. Histol Histopathol 15 (2000) 825-834
27. Oda Y., Muroishi Y., Misawa H., and Suzuki S. Comparative study of gene expression of cholinergic system-related molecules in the human spinal cord and term placenta. Neuroscience 128 (2004) 39-49
28. Ogawa H., Fujii T., Watanabe Y., and Kawashima K. Expression of multiple mRNA species for choline acetyltransferase in human T-lymphocytes. Life Sci 72 (2003) 2127-2130
29. Oh J.D., Woolf N.J., Roghani A., Edwards R.H., and Butcher L.L. Cholinergic neurons in the rat central nervous system demonstrated by in situ hybridization of choline acetyltransferase mRNA. Neuroscience 47 (1992) 807-822
30. Paxinos G., and Franklin K.B.J. The mouse brain in stereotaxic coordinates. 2nd ed. (2001), Academic Press, San Diego
31. Pfeil U., Vollerthun R., Kummer W., and Lips K. Expression of the cholinergic gene locus in the rat placenta. Histochem Cell Biol 122 (2004) 121-130
32. Schliebs R., and Arendt T. The significance of the cholinergic system in the brain during aging and in Alzheimer's disease. J Neural Transm 113 (2006) 1625-1644
33. Schutz B., Weihe E., and Eiden L.E. Independent patterns of transcription for the products of the rat cholinergic gene locus. Neuroscience 104 (2001) 633-642
34. Strada O., Vyas A., Hirsch E., Ruberg M., Brice A., Agid Y., and Javoy-Agid F. Decreased choline acetyltransferase mRNA expression in the nucleus basalis of Meynert in Alzheimer's disease: an in situ hybridization study. Proc Natl Acad Sci U S A 89 (1992) 9549-9553
35. Timmusk T., Lendahl U., Funakoshi H., Arenas E., Persson H., and Metsis M. Identification of brain-derived neurotrophic factor promoter regions mediating tissue-specific, axotomy-, and neuronal activity-induced expression in transgenic mice. J Cell Biol 128 (1995) 185-199
36. Tooyama I., and Kimura H. A protein encoded by an alternative splice variant of choline acetyltransferase mRNA is localized preferentially in peripheral nerve cells and fibers. J Chem Neuroanat 17 (2000) 217-226
37. Vandenbroucke II V.J., Paepe A.D., and Messiaen L. Quantification of splice variants using real-time PCR. Nucleic Acids Res 29 (2001) E68-E68
38. Virgo L., de Belleroche J., Rossi M., and Steiner T.J. Characterization of the distribution of choline acetyltransferase messenger RNA in human spinal cord and its depletion in motor neuron disease. J Neurol Sci 112 (1992) 126-132
39. von Engelhardt J., Eliava M., Meyer A.H., Rozov A., and Monyer H. Functional characterization of intrinsic cholinergic interneurons in the cortex. J Neurosci 27 (2007) 5633-5642
40. Woolf N. Cholinergic systems in mammalian brain and spinal cord. Prog Neurobiol 37 (1991) 475-524
41. Wu D., and Hersh B. Choline acetyltransferase: celebrating its fiftieth year. J Neurochem 62 (1994) 1653-1663