A SELDI-TOF MS procedure for the detection, quantitation, and preliminary characterization of low-molecular-weight recombinant proteins expressed in transgenic plants

Proteomics

Volumn 9, Issue 2, 2009, Pages 233-241

  Badri, M Amine ^a   Rivard, Daniel ^a   Coenen, Karine ^a   Vaillancourt, Louis Philippe ^a   Goulet, Charles ^a   Michaud, Dominique ^a,b  

^a UNIVERSITÉ LAVAL   (Canada)

^b Pavilion des Services (INAF)   (Canada)

Author keywords

Bovine aprotinin; Corn cystatin ii; Recombinant protein quantitation; Surface enhanced laser desorption ionization time of flight mass spectrometry (SELDI TOF MS); Transgenic plants

Indexed keywords

APROTININ; CYSTATIN B; RECOMBINANT PROTEIN;

ARTICLE; BIOCHIP; CATION EXCHANGE; CELL COMPARTMENTALIZATION; ENZYME IMMOBILIZATION; GENE EXPRESSION; IMMUNOASSAY; IMMUNOBLOTTING; IMMUNODETECTION; INTRACELLULAR SPACE; MOLECULAR WEIGHT; NONHUMAN; POTATO; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RHIZOBIUM RADIOBACTER; SURFACE ENHANCED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; TRANSGENE; TRANSGENIC PLANT;

AMINO ACID SEQUENCE; ANIMALS; APROTININ; CATTLE; CYSTATINS; ENDOPLASMIC RETICULUM; GENE EXPRESSION; LEAST-SQUARES ANALYSIS; MOLECULAR SEQUENCE DATA; PLANT LEAVES; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; RHIZOBIUM RADIOBACTER; SOLANUM TUBEROSUM; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

AGROBACTERIUM TUMEFACIENS; BOVINAE; SOLANUM TUBEROSUM; ZEA MAYS;

EID: 60349086202     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.200700233     Document Type: Article

References (33)

1. Walsh, G., Biopharmaceutical benchmarks-2003. Nat. Biotechnol. 2003, 21, 865-870.
2. Baneyx, F., Mujacic, M., Recombinant protein folding and misfolding in Escherichia coli. Nat. Biotechnol. 2004, 22, 1399-1408.
3. Gerngross, T. U., Advances in the production of human therapeutic proteins in yeasts and filamentous fungi. Nat. Biotechnol. 2004, 22, 1409-1414.
4. Wurm, F. M., Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 2004, 22, 1393-1398.
5. Dyck, M. K., Lacroix, D., Pothier, F., Sirard, M. A., Making recombinant proteins in animals - different systems, different applications. Trends Biotechnol. 2003, 21, 394-399.
6. Ma, J. K., Drake, P. M., Christou, P., The production of recombinant pharmaceutical proteins in plants. Nat. Rev. Genet. 2003, 4, 794-805.
7. Daniell, H., Streatfield, S. J., Wycoff, K., Medical molecular farming: Production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends PlantSci. 2001, 6, 219-226.
8. Twyman, R. M., Stoger, E., Schillberg, S., Christou, P., Fischer, R., Molecular farming in plants: Host systems and expression technology. Trends Biotechnol. 2003, 21, 570-578.
9. Gomord, V., Sourrouille, C., Fitchette, A. C., Bardor, M. et al., Production and glycosylation of plant-made pharmaceuticals: The antibodies as a challenge. Plant Biotechnol. J. 2004, 2, 83-100.
10. Yoshida, K., Shinmyo, A., Transgene expression systems in plant, a natural bioreactor. J. Biosci. Bioeng. 2000, 90, 353-362.
11. Potenza, C., Aleman, L., Sengupta-Gopalan, C., Targeting transgene expression in research, agricultural, and environmental applications: Promoters used in plant transformation. In Vitro Cell. Dev. Biol. Plant 2004, 40, 1-22.
12. Gomord, V., Faye, L., Posttranslational modification of therapeutic proteins in plants. Curr. Opin. Plant Biol. 2004, 7, 171-181.
13. Faye, L., Boulaflous, A., Benchabane, M., Gomord, V., Michaud, D., Protein modifications in the plant secretory pathway: Current status and practical implications in molecular pharming. Vaccine 2005, 23, 1770-1778.
14. Benchabane, M., Goulet, C., Rivard, D., Faye, L., Gomord, V., Michaud, D., Preventing unintended proteolysis in plant protein biofactories. Plant Biotechnol. J. 2008, 6, 633-648.
15. Chirino, A. J., Mire-Sluis, A., Characterizing biological products and assessing comparability following manufacturing changes. Nat. Biotechnol. 2004, 22, 1383-1391.
16. Ascenzi, P., Bocedi, A., Bolognesi, M., Spallarossa, A. et al., The bovine basic pancreatic trypsin inhibitor (Kunitz inhibitor): A milestone protein. Curr. Prot. Pept. Sci. 2003, 4, 231-251.
17. Abe, M., Abe, K., Domoto, C., Arai, S., Two distinct species of corn cystatin in corn kernels. Biosci. Biotechnol. Biochem. 1995, 59, 756-758.
18. Tang, N., Tornatore, P., Weinberger, S. R., Current developments in SELDI affinity technology. Mass Spectrom. Rev. 2004, 23, 34-44.
19. Rivard, D., Anguenot, A., Brunelle, F., Le, V. Q. et al.,An inbuilt proteinase inhibitor system for the protection of recombinant proteins recovered from transgenic plants. Plant Biotechnol. J. 2006, 4, 359-368.
20. Shorrosh, B. S., Dixon, R. A., Molecular cloning of a putative plant endomembrane protein resembling vertebrate protein disulfide-isomerase and a phosphatidylinositol specific phospholipase C. Proc. Natl. Acad. Sci. USA 1991, 88, 10941-10945.
21. Darveau, A., Pelletier, A., Perreault, J., PCR-mediated synthesis of chimeric molecules. Meth. Neurosci. 1995, 26,77-85.
22. Brunelle, F., Girard, C., Cloutier, C., Michaud, D., A hybrid, broad-spectrum inhibitor of Colorado potato beetle aspartate and cysteine digestive proteinases. Arch. Insect Biochem. Physiol. 2005, 60, 20-31.
23. Vaillancourt, L.-P., Effets pléïotropiques d'une forme recombinante de la cystatine II du maïs exprimée dans le cytosol des cellules de la pomme de terre. M.Sc. Thesis, Université Laval, Quebec QC, Canada, 2005.
24. Michaud, D., Vrain, T. C., Expression of recombinant proteinase inhibitors in plants. Meth. Biotechnol. 1998, 3, 49-64.
25. Edwards, K., Johnstone, C., Thompson, C., A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucl. Acids Res. 1991, 19, 1349.
26. Pfaffl, M. W., Tichopad, A., Prgomet, C., Neuvians, T. P., Realtime RT-PCR quantification of insulin-like growth factor (IGF)-1, IGF-1 receptor, IGF-2, IGF-2 receptor, insulin receptor, growth hormone receptor, IGF-binding protein 1, 2 and 3 in the bovine species. Biotechnol. Lett. 2004, 26, 509-515.
27. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
28. Schägger, H., Tricine-SDS-PAGE. Nat. Protocols 2006, 1,16-22.
29. Bouchard, É., Cloutier, C., Michaud, D., Oryzacystatin I expressed in transgenic potato induces digestive compensation in an insect natural predator via its herbivorous prey feeding on the plant. Mol. Ecol. 2003, 12, 2439-2446.
30. Vinther, A., Bjorn, S. E., Holmegaard Sorensen, H., Soeberg, H., Identification of aprotinin degradation products by the use of high-performance capillary electrophoresis, high pressure liquid chromatography and mass spectrometry. J. Chromatogr. 1990, 516, 175-184.
31. Gattiker, A., Bienvenut, W. V., Bairoch, A., Gasteiger, E., FindPept, a tool to identify unmatched masses in peptide mass fingerprinting protein identification. Proteomics 2002, 2, 1435-1444.
32. Vorderwülbecke, S., Cleverly, S., Weinberger, S. R., Wiesner, A., Protein quantification by the SELDI-TOF-MS-based ProteinChip System. Nat. Meth. 2005, 2, 393-395.
33. Bradford, M. M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.