3-Carboxy-6-chloro-7-hydroxycoumarin: A highly fluorescent, water-soluble violet-excitable dye for cell analysis

Analytical Biochemistry

Volumn 386, Issue 2, 2009, Pages 262-269

  Abrams, Barny ^a   Diwu, Zhenjun ^b   Guryev, Oleg ^a   Aleshkov, Sergei ^a   Hingorani, Ravi ^a   Edinger, Mark ^a   Lee, Rita ^a   Link, Joe ^a   Dubrovsky, Tim ^a  

^a BD BIOSCIENCES   (United States)

^b ABDBioquest   (United States)

Author keywords

Cell analysis; Immunophenotyping; Lymphocytes; Multicolor flow cytometry; Subsetting; Violet excitable dye

Indexed keywords

FLOW CYTOMETRY; LYMPHOCYTES; MONOCLONAL ANTIBODIES;

ANTIBODY CONJUGATE; CELL ANALYSIS; FLOW CYTOMETRY ASSAYS; HYDROXYCOUMARINS; IMMUNOPHENOTYPING; PHOTOCHEMICAL PROPERTIES; RELIABLE PERFORMANCE; SUBSETTING;

T-CELLS;

COUMARIN DERIVATIVE; DYE; HALOGEN; MONOCLONAL ANTIBODY; MONOCLONAL ANTIBODY CD3; MONOCLONAL ANTIBODY CD45; OKT 4; REAGENT; UNCLASSIFIED DRUG; WATER;

ANALYSIS; ARTICLE; BLEACHING; BONE MARROW CELL; COLOR; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LEUKOCYTE; LYMPHOCYTE; PRIORITY JOURNAL; QUANTUM YIELD;

EID: 59949093499     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.12.018     Document Type: Article

References (13)

1. Lavis L.D., and Raines R.T. Bright ideas for chemical biology. ACS Chem. Biol. 3 (2008) 142-155
2. Telford W.G., Hawley T.S., and Hawley R.G. Analysis of violet-excited fluorochromes by flow cytometry using a violet laser diode. Cytometry A 54 (2003) 48-55
3. Shapiro H.M., and Perlmutter N.G. Violet laser diodes as light sources for cytometry. Cytometry 44 (2001) 133-136
4. Kilkarni M.V., Kilkarni G.M., Lin C.-H., and Sun C.-M. Recent advances in coumarins and 1-azocoumarins as versatile biodynamic agents. Curr. Med. Chem. 13 (2006) 2795-2818
5. Whittaker J.E., Haugland R.P., Moore P.L., Hewitt P.C., Reese M., and Haugland R.P. Cascade blue derivatives: water soluble, reactive blue emission dyes evaluated as fluorescent labels and tracers. Anal. Biochem. 198 (1991) 119-130
6. Anderson M.T., Baumgarth N., Haugland R.P., Gerstein R.M., Tjioe T., Herzenberg L.A., and Herzenberg L.A. Pairs of violet light excited fluorochromes for flow cytometry analysis. Cytometry 33 (1998) 435-444
7. Telford W., Kapoor V., Jackson J., Burgess W., Buller G., Hawley T., and Hawley R. Violet laser diodes in flow cytometry: an update. Cytometry A 69 (2006) 1153-1160
8. Zlokarnik G., Negulescu P.A., Knapp T.E., Mere L., Burres N., Feng L., Whitney M., Roemer K., and Tsien R.Y. Quantitation of transcription and clonal selection of single living cells with β-lactamase as reporter. Science 279 (1998) 84-88
9. Sun W.-C., Gee K.R., and Haugland R.P. Synthesis of novel fluorinated coumarins: excellent UV light-excitable fluorescent dyes. Bioorg. Med. Chem. Lett. 8 (1998) 3107-3110
10. K.R. Gee, R.P. Haugland, W.-C. Sun, Derivatives of 6,8-difluoro-7-hydroxycoumarin, U.S. patent 5,830,912 (1998).
11. Panchuk-Voloshina N., Haugland R.P., Bishop-Stewart J., Bhalgat M.K., Millard P.J., Mao F., Leung W.-Y., and Haugland R.P. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. J. Histochem. Cytochem. 47 (1999) 1179-1188
12. Velapoldi R., and Tonnensen H. Corrected emission spectra and quantum yields for a series of fluorescent compounds in the visible spectral region. J. Fluoresc. 14 (2004) 465-472
13. Raju B.B., and Varadarajan T.S. Spectroscopic studies of 7-diethylamino-3-styryl coumarins. J. Photochem. Photobiol. 85 (1995) 263-267