Analysis of Violet-Excited Fluorochromes by Flow Cytometry Using a Violet Laser Diode

Cytometry Part A

Volumn 54, Issue 1, 2003, Pages 48-55

  Telford, William G ^a,c   Hawley, Teresa S ^b   Hawley, Robert G ^b  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b HOLLAND LABORATORY   (United States)

^c NATIONAL CANCER INSTITUTE   (United States)

Author keywords

2 (5 chloro 2 phosphoryloxy phenyl) 6 chloro 4 (3H) quinazoline; Cascade Blue; Cyan fluorescent protein; Pacific Blue; Violet laser diode

Indexed keywords

2 (5' CHLORO 2' PHOSPHORYLOXYPHENYL) 6 CHLORO 4(3H) QUINAZOLINE; 4,6 DIAMIDINO 2 PHENYLINDOLE; ALKALINE PHOSPHATASE; ANTIBODY CONJUGATE; CASCADE BLUE; CYAN FLUORESCENT PROTEIN; DISCOSOMA RED FLUORESCENT PROTEIN; DYE; FLUOROCHROME; GREEN FLUORESCENT PROTEIN; PACIFIC BLUE; UNCLASSIFIED DRUG;

ANALYZER; ANIMAL CELL; ANTIBODY LABELING; ARGON LASER; ARTICLE; CELL LABELING; CELL SURFACE; COMPARATIVE STUDY; CYTOMETER; DIODE LASER; DNA CONTENT; EXCITATION; FLOW CYTOMETRY; FLUORESCENCE; KRYPTON LASER; LASER; MULTICOLOR FLUORESCENT PROTEIN ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; SIGNAL NOISE RATIO; VIOLET LASER DIODE; VIOLET LASER EXCITATION; WATER COOLED GAS LASER;

EID: 0347706928     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.10046     Document Type: Article

References (21)

1. Shapiro H. Practical flow cytometry. New York: John Wiley & Sons; 1995.
2. Nakamura S, Fasol G. The blue laser diode. GaN based light emitters and lasers. Berlin: Springer-Verlag; 1997.
3. Shapiro HM, Perlmutter NG. Violet laser diodes as light sources for cytometry. Cytometry 2001;44:133-136.
4. Cheng L, Du C, Murray D, Tong X, Zhang YA, Chen BP, Hawley RG. A GFP reporter system to assess gene transfer and expression in viable human hematopoietic progenitors. Gene Ther 1997;4:1013-1022.
5. Hawley RG, Lieu FHL, Fong AZC, Hawley TS. Versatile retroviral vectors for potential use in gene therapy. Gene Ther 1994;1:136-138.
6. Hawley RG, Lieu FHL, Fong AZC, Goldman SJ, Leonard JP, Hawley TS. Retroviral vectors for production of interleukin-12 in the bone marrow to induce a graft-versus-leukemia effect. Ann NY Acad Sci 1996;795:341-345.
7. Hawley RG, Hawley TS, Fong AZC, Quinto C, Collins M, Leonard JP, Goldman SJ. Thrombopoietic potential and serial repopulating ability of murine hematopoietic stem cells constitutively expressing interleukin-11. Proc Nati Acad Sci USA 1996;93:10297-10302.
8. Whittaker JE, Haugland RP, Moore PL, Hewitt PC, Reese M, Haugland RP. Cascade Blue derivatives: water soluble, reactive blue emission dyes evaluated as fluorescent labels and tracers. Anal Biochem 1991;198:119-130.
9. Anderson MT, Baumgarth N, Haugland RP, Gerstein RM, Tjioe T, Herzenberg LA, Herzenberg LA. Pairs of violet-light-excited fluorochromes for flow cytometric analysis. Cytometry 1998;33:435-444.
10. Baumgarth N, Roederer M. A practical approach to multicolor flow cytometry for immunophenotyping. J Immunol Methods 2000;243:77-97.
11. Beavis A, Pennline K. Simultaneous measurement of five cell surface antigens by five color immunofluorescence. Cytometry 1994;15:371-376.
12. Roederer M, DeRosa S, Gerstein R, Anderson M, Bigos M, Stovel R, Nozaki T, Parks D, Herzenberg L, Herzenberg L. 8-color, 10 -parameter flow cytometry: elucidation of complex leukocyte heterogeneity. Cytometry 1997;29:1-12.
13. Ropp JD, Donahue CJ, Wolfgang-Kimball D, Hooley JJ, Chin JY, Cuthbertson RA. Bauer KD. Aequora green fluorescent protein: simultaneous analysis of wild-type and blue-fluorescing mutant by flow cytometry. Cytometry 1996;24:284-288.
14. Heim R, Tsien RY. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr Biol 1996;1:178-182.
15. Beavis AJ, Kalejta RF. Simultaneous analysis of the cyan, yellow and green fluorescent proteins by flow cytometry using single-laser excitation at 458 nm. Cytometry 1999;37:68-73.
16. Hawley TS, Telford WG, Ramezani A, Hawley RG. Four-color flow cytometric detection of retrovirally expressed red, yellow, green and cyan fluorescent proteins. Biotechniques 2001;30:1028-1034.
17. Hawley TS, Telford WG, Ramezani A, Hawley RG. "Rainbow" reporters for multispectral marking and lineage analysis of hematopoietic stem cells. Stem Cells 2001;19:118-124.
18. Singer VL, Paragas VB, Larison KD, Wells KS, Fox CJ, Haugland RP. Fluorescence-based signal amplification technology. Am Biotech Lab 1994;12:55-58.
19. Haugland RP, Johnson ID. Detecting enzymes in living cells using fluorogenic substrates. J Fluoresc 1993;3:119-127.
20. Telford WG, Cox WG, Stiner D, Singer VL, Doty SM. Detection of endogenous alkaline phosphatase activity in intact cells by flow cytometry using the fluorogenic ELF-97 phosphatase substrate. Cytometry 1999;37:314-319.
21. Telford WG, Cox WG, Singer VL. Detection of endogenous and antibody-conjugated alkaline phosphatase with ELF-97 phosphate in multicolor flow cytometry applications. Cytometry 2001;43:117-125.