Chapter 9 Biochemical and Morphological Detection of Inclusion Bodies in Autophagy-Deficient Mice

Methods in Enzymology

Volumn 453, Issue C, 2009, Pages 181-196

  Waguri, Satoshi ^a   Komatsu, Masaaki ^b  

^a FUKUSHIMA MEDICAL UNIVERSITY   (Japan)

^b TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEASOME; PROTEIN P62; UBIQUITIN; GTF2H1 PROTEIN, MOUSE; TRANSCRIPTION FACTOR;

ALZHEIMER DISEASE; AMYOTROPHIC LATERAL SCLEROSIS; AUTOPHAGY; BRAIN TISSUE; CELL INCLUSION; CELL ISOLATION; CELL LYSATE; CRYOPRECIPITATE; ELECTRON MICROSCOPY; IMMUNOFLUORESCENCE MICROSCOPY; IMMUNOHISTOCHEMISTRY; LIVER; LIVER CELL CARCINOMA; NONHUMAN; PARKINSON DISEASE; PATHOPHYSIOLOGY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; REVIEW; TISSUE FIXATION; TISSUE SECTION; UBIQUITINATION; WESTERN BLOTTING; ANIMAL; ARTICLE; GENETICS; METABOLISM; MOUSE; MOUSE MUTANT; PHYSIOLOGY;

MUS;

ANIMALS; AUTOPHAGY; IMMUNOHISTOCHEMISTRY; INCLUSION BODIES; MICE; MICE, MUTANT STRAINS; TRANSCRIPTION FACTORS; UBIQUITIN;

EID: 59849126863     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(08)04009-3     Document Type: Review

References (26)

1. Aono J., Yanagawa T., Itoh K., Li B., Yoshida H., Kumagai Y., Yamamoto M., and Ishii T. Activation of Nrf2 and accumulation of ubiquitinated A170 by arsenic in osteoblasts. Biochem. Biophys. Res. Commun. 305 (2003) 271-277
2. Bergamini E. Autophagy: A cell repair mechanism that retards ageing and age-associated diseases and can be intensified pharmacologically. Mol. Aspects. Med. 27 (2006) 403-410
3. Bjørkøy G., Lamark T., Brech A., Outzen H., Perander M., Øvervatn A., Stenmark H., and Johansen T. p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death. J. Cell. Biol. 171 (2005) 603-614
4. Boland B., Kumar A., Lee S., Platt F.M., Wegiel J., Yu W.H., and Nixon R.A. Autophagy induction and autophagosome clearance in neurons: Relationship to autophagic pathology in Alzheimer's disease. J. Neurosci. 28 (2008) 6926-6937
5. Goldberg A.L. Protein degradation and protection against misfolded or damaged proteins. Nature 426 (2003) 895-899
6. Haglund K., Sigismund S., Polo S., Szymkiewicz I., Di Fiore P.P., and Dikic I. Multiple monoubiquitination of RTKs is sufficient for their endocytosis and degradation. Nat. Cell Biol. 5 (2003) 461-466
7. Hara T., Nakamura K., Matsui M., Yamamoto A., Nakahara Y., Suzuki-Migishima R., Yokoyama M., Mishima K., Saito I., Okano H., and Mizushima N. Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice. Nature. 441 (2006) 885-889
8. Ichimura Y., Kumanomidou T., Sou Y.S., Mizushima T., Ezaki J., Ueno T., Kominami E., Yamane T., Tanaka K., and Komatsu M. Structural basis for sorting mechanism of p62 in selective autophagy. J. Biol. Chem. 283 (2008) 22847-22857
9. Ishii T., Yanagawa T., Kawane T., Yuki K., Seita J., Yoshida H., and Bannai S. Murine peritoneal macrophages induce a novel 60-kDa protein with structural similarity to a tyrosine kinase p56lck-associated protein in response to oxidative stress. Biochem. Biophys. Res. Commun. 226 (1996) 456-460
10. Itoh T., Fujita N., Kanno E., Yamamoto A., Yoshimori T., and Fukuda M. Golgi-resident small GTPase Rab33B interacts with Atg16L and modulates autophagosome formation. Mol. Biol. Cell. 19 (2008) 2916-2925
11. Komatsu M., Waguri S., Chiba T., Murata S., Iwata J., Tanida I., Ueno T., Koike M., Uchiyama Y., Kominami E., and Tanaka K. Loss of autophagy in the central nervous system causes neurodegeneration in mice. Nature 441 (2006) 880-884
12. Komatsu M., Waguri S., Koike M., Sou Y.S., Ueno T., Hara T., Mizushima N., Iwata J., Ezaki J., Murata S., Hamazaki J., Nishito Y., et al. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 131 (2007) 1149-1163
13. Komatsu M., Waguri S., Ueno T., Iwata J., Murata S., Tanida I., Ezaki J., Mizushima N., Ohsumi Y., Uchiyama Y., Kominami E., Tanaka K., et al. Impairment of starvation-induced and constitutive autophagy in Atg7-deficient mice. J. Cell Biol. 169 (2005) 425-434
14. Kuusisto E., Kauppinen T., and Alafuzoff I. Use of p62/SQSTM1 antibodies for neuropathological diagnosis. Neuropathol. Appl. Neurobiol. 34 (2008) 169-180
15. Lowe J., Hand N., and Mayer R.J. Application of ubiquitin immunohistochemistry to the diagnosis of disease. Methods. Enzymol. 399 (2005) 86-119
16. Nakai A., Yamaguchi O., Takeda T., Higuchi Y., Hikoso S., Taniike M., Omiya S., Mizote I., Matsumura Y., Asahi M., Nishida K., Hori M., et al. The role of autophagy in cardiomyocytes in the basal state and in response to hemodynamic stress. Nat. Med. 13 (2007) 619-624
17. Nakano T., Nakaso K., Nakashima K., and Ohama E. Expression of ubiquitin-binding protein p62 in ubiquitin-immunoreactive intraneuronal inclusions in amyotrophic lateral sclerosis with dementia: Analysis of five autopsy cases with broad clinicopathological spectrum. Acta Neuropathol. 107 (2004) 359-364
18. Nezis I.P., Simonsen A., Sagona A.P., Finley K., Gaumer S., Contamine D., Rusten T.E., Stenmark H., and Brech A. Ref(2)P, the Drosophila melanogaster homologue of mammalian p62, is required for the formation of protein aggregates in adult brain. J. Cell. Biol. 180 (2008) 1065-1071
19. Pankiv S., Clausen T.H., Lamark T., Brech A., Bruun J.A., Outzen H., Øvervatn A., Bjørkøy G., and Johansen T. p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy. J. Biol. Chem. 282 (2007) 24131-24145
20. Pickford F., Masliah E., Britschgi M., Lucin K., Narasimhan R., Jaeger P.A., Small S., Spencer B., Rockenstein E., Levine B., and Wyss-Coray T. The autophagy-related protein beclin 1 shows reduced expression in early Alzheimer disease and regulates amyloid β accumulation in mice. J. Clin. Invest. 118 (2008) 2190-2199
21. Stumptner C., Fuchsbichler A., Heid H., Zatloukal K., and Denk H. Mallory body-a disease-associated type of sequestosome. Hepatology 35 (2002) 1053-1062
22. Tokuyasu K.T. Application of cryoultramicrotomy to immunocytochemistry. J. Microsc. 143 (1986) 139-149
23. Ueno T., Watanabe S., Hirose M., Namihisa T., and Kominami E. Phalloidin-induced accumulation of myosin in rat hepatocytes is caused by suppression of autolysosome formation. Eur. J. Biochem. 190 (1990) 63-69
24. Wang Q.J., Ding Y., Kohtz D.S., Mizushima N., Cristea I.M., Rout M.P., Chait B.T., Zhong Y., Heintz N., and Yue Z. Induction of autophagy in axonal dystrophy and degeneration. J. Neurosci. 26 (2006) 8057-8068
25. Wooten M.W., Hu X., Babu J.R., Seibenhener M.L., Geetha T., Paine M.G., and Wooten M.C. Signaling, polyubiquitination, trafficking, and inclusions: Sequestosome 1/p62's role in neurodegenerative disease. J. Biomed. Biotechnol. 2006 (2006) 62079
26. Zatloukal K., Stumptner C., Fuchsbichler A., Heid H., Schnoelzer M., Kenner L., Kleinert R., Prinz M., Aguzzi A., and Denk H. p62 Is a common component of cytoplasmic inclusions in protein aggregation diseases. Am. J. Pathol. 160 (2002) 255-263