Development of real-time PCR assays in the study of gonadotropin subunits, follistatin and prolactin genes expression in the porcine anterior pituitary during the preovulatory period

Neuroendocrinology Letters

Volumn 29, Issue 6, 2008, Pages 958-964

  Lisowski, Pawel ^a   Robakowska Hyzorek, Dagmara ^a   Blitek, Agnieszka ^b   Kaczmarek, Monika M ^b   Gajewska, Alina ^c   Kochman, Helena ^c   Zwierzchowski, Lech ^a   Ziecik, Adam J ^b   Kochman, Kazimierz ^c  

^a INSTITUTE OF GENETICS AND ANIMAL BREEDING   (Poland)

^b INSTITUTE OF ANIMAL REPRODUCTION AND FOOD RESEARCH   (Poland)

^c KIELANOWSKI INSTITUTE OF ANIMAL PHYSIOLOGY AND NUTRITION   (Poland)

Author keywords

Anterior pituitary; Gonadotropin releasing hormone; Real time PCR normalization; Reference gene; Reproduction

Indexed keywords

BETA 2 MICROGLOBULIN; BETA ACTIN; CHORIONIC GONADOTROPIN ALPHA SUBUNIT; DNA TOPOISOMERASE (ATP HYDROLYSING); FOLLISTATIN; FOLLITROPIN BETA SUBUNIT; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE (NADP); GONADORELIN; GONADORELIN ANTAGONIST; LUTEINIZING HORMONE; MESSENGER RNA; PROLACTIN; RECOMBINANT FOLLITROPIN; RIBOSOME PROTEIN;

ADENOHYPOPHYSIS; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CONTROLLED STUDY; GENE EXPRESSION; GENETIC TRANSCRIPTION; GONADOTROPIN RELEASE; HORMONE SYNTHESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OVARY CYCLE; PROTEIN STRUCTURE; QUANTITATIVE ANALYSIS; REGULATORY MECHANISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 59849094068     PISSN: 0172780X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Barnea A, Cho G. Evidence that copper-amino acid complexes are potent stimulators of the release of luteinizing hormone-releasing hormone from isolated hypothalamic granules. Endocrinology 1984; 115, 936-43.
2. Bereta J., Bereta M. Stimulation of glyceraldehyde-3-phosphate dehydrogenase mRNA levels by endogenous nitric oxide in cytokine-activated endothelium. Biochem Biophys Res Commun 1995; 217: 363-9.
3. Blitek A, Lisowski P, Robakowska-Hyzorek D, Gajewska A, Kaczmarek MM, Counis R, Zwierzchowski L, Ziecik AJ, Kochman K. Cobalt complex with GnRH slightly stimulates the follistatin gene expression in the pig anterior pituitary only after long time incubation, in vitro. 17th International Symposium on "Molecular and Physiological Aspects of Regulatory Processes in the Organism", Cracow, 2008; June 5-6, Abstract: pp. 56-58.
4. Blitek A, Ziecik A, Gajewska A, Kodaka M, Counis R, Kochman K. Cobalt complex with GnRH stimulates the LH release and PKA signaling pathway in pig anterior pituitary cells in vitro. Brain Res Bull 2005; 65 :391-6.
5. Braud VM, Allan DS, McMichael AJ. Functions of nonclassical MHC and non-MHC-encoded class I molecules. Curr Opin Immunol 1999; 11, 100-8.
6. Burrows GH, Barnea A. Copper stimulates the release of luteinizing hormone releasing hormone from isolated hypothalamic granules. Endocrinology 1982; 110: 1456-8.
7. Bustin SA. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J Mol Endocrinol 2002; 29: 23-39.
8. Chang GW, Terzaghi-Howe M. Multiple changes in gene expression are associated with normal cell-induced modulation of the neoplastic phenotype. Cancer Res 1998; 58: 4445-52.
9. Colombani-Vidal M, Barnea A. Copper stimulation of LHRH release from median eminence explants. I. A divalent metal specific process that does not require extracellular calcium. Neuroendocrinology 1986a; 43: 664-9.
10. Colombani-Vidal M, Barnea A. Copper stimulation of LHRH release from median eminence explants. II. A process dependent on chloride transport. Neuroendocrinology 1986b; 44: 276-82.
11. Colombani-Vidal M, Barnea A. Copper stimulation of LHRH release from median eminence explants. III. A process dependent on extracellular sodium. Neuroendocrinology 1986c; 44: 283-91.
12. Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, Zumla A. Validation of housekeeping genes for normalizing RNA expression in real-time PCR. Biotechniques 2004; 37: 112-4, 116, 118-9.
13. Erkens T, Van Poucke M, Vandesompele J, Goossens K, Van Zeveren A, Peelman LJ. Development of a new set of reference genes for normalization of real-time RT-PCR data of porcine backfat and longissimus dorsi muscle, and evaluation with PPARGC1A. BMC Biotechnol 2006; 6: 41.
14. Evans WS, Uskavitch DR, Kaiser DL, Hellmann P, Borges JL,Thorner MO. The self-priming effect of gonadotropin-releasing hormone on luteinizing hormone release: observations using rat anterior pituitary fragments and dispersed cells continuously perifused in parallel. Endocrinology 1984; 114, 861-7.
15. Fink L, Seeger W, Ermert L, Hanze J, Stahl U, Grimminger F, Kummer W, Bohle RM. Real-time quantitative RT-PCR after laser-assisted cell picking. Nat Med 1998; 4: 1329-33.
16. Gajewska A, Wolinska-Witort E, Kochman K. Vasoactive intestinal peptide modulates luteinizing hormone subunit gene expression in the anterior pituitary in the female rat. Brain Res Bull 2005; 67 (4): 319-326.
17. Gharib SD, Leung PC, Carroll RS, Chin WW. Androgens positively regulate follicle-stimulating hormone beta-subunit mRNA levels in rat pituitary cells. Mol Endocrinol 1990; 4: 1620-6.
18. Hazum E. Copper and thiol regulation of gonadotropin releasing hormone binding and luteinizing hormone release. Biochem Biophys Res Commun 1983; 112: 306-12.
19. Hiatt ES, Schwartz NB. Suppression of basal and GnRH-stimulated gonadotropin secretion rate in vitro by GnRH antagonist: differential effects on metestrous and proestrous pituitaries. Neuroendocrinology 1989; 50: 158-64.
20. Holland NT, Smith MT, Eskenazi B, Bastaki M. Biological sample collection and processing for molecular epidemiological studies. Mutat Res 2003; 543, 217-34.
21. Jemiolo B., Trappe S. Single muscle fiber gene expression in human skeletal muscle: validation of internal control with exercise. Biochem Biophys Res Commun 2004; 320: 1043-50.
22. Kaiser UB, Sabbagh E, Katzenellenbogen RA, Conn PM, Chin WW. A mechanism for the differential regulation of gonadotropin subunit gene expression by gonadotropin-releasing hormone. Proc Natl Acad Sci USA 1995; 92: 12280-4.
23. Kochman K, Blitek A, Kaczmarek M, Gajewska A, Siawrys G, Counis R, Ziecik AJ. Different signaling in pig anterior pituitary cells by GnRH and its complexes with copper and nickel. Neuro Endocrinol Lett 2005; 26: 377-82.
24. Kochman K, Gajewska A, Kozlowski H, Masiukiewicz E, Rzeszotarska B. Increased LH and FSH release from the anterior pituitary of ovariectomized rat, in vivo, by copper-, nickel-, and zinc-LHRH complexes. J Inorg Biochem 1992; 48: 41-6.
25. Kochman K, Gajewska A, Michaluk A, Kulon K, Swiatek-Kozlowska J, Kozlowski H. Copper-GnRH complex stability in hypothalamic and pituitary tissue, in vitro. 9th International Symposium on GnRH, Berlin, 2008; February 10-12. Abstract: p. 65..
26. Michaluk A, Blitek A, Gajewska A, Kaczmarek MM, Gromadzka-Hliwa K, Kochman H, Ziecik AJ, Kochman K. LH release by Cu and Ni salts and metal-GnRH complexes, in vitro. Neuro Endocrinol Lett 2006; 27: 483-6.
27. Peters IR, Peeters D, Helps CR, Day MJ. Development and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies. Vet Immunol Immunopathol 2007; 117: 55-66.
28. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001; 29: e45.
29. Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res 2002; 30: e36.
30. Ratcliffe KE, Fraser HM, Sellar R, Rivier J, Millar RP. Bifunctional gonadotropin-releasing hormone antagonist-progester-one analogs with increased efficacy and duration of action. Endocrinology 2006; 147: 571-9.
31. Robakowska-Hyzorek D, Blitek A, Lisowski P, Gajewska A, Kaczmarek MM, Ziecik AJ, Zwierzchowski L, Kochman K. Modulation of follistatin gene expression by Cu-GnRH complex in the pig anterior pituitary, in vitro. 17th International Symposium on "Molecular and Physiological Aspects of Regulatory Processes in the Organism", Cracow, 2008; June 5-6, Abstract: pp. 442-444.
32. Solheim JC. Class I MHC molecules: assembly and antigen presentation. Immunol Rev 1999; 172: 11-9.
33. Sutton-Walsh HG, Whelly S, Cornwall GA. Differential effects of GnRH and androgens on Cres mRNA and protein in mouse anterior pituitary gonadotropes. J Andrology 2006; 27: 802-815.
34. Svobodova K, Bilek K, Knoll A. Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig. J Appl Genet 2008; 49: 263-5.
35. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 2002; 3: RESEARCH0034.
36. Walker NJ. Tech. Sight. A technique whose time has come. Science 2002; 296: 557-9.
37. Zhong H, Simons JW. Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia. Biochem Biophys Res Commun 1999; 259: 523-6.