Continuous flow assay for the simultaneous measurement of the electrophoretic mobility, catalytic activity and its variation over time of individual molecules of Escherichia coli β-galactosidase

Electrophoresis

Volumn 29, Issue 21, 2008, Pages 4298-4303

  Craig, Douglas B ^a   Nichols, Ellert R ^b  

^a UNIVERSITY OF WINNIPEG   (Canada)

^b UNIVERSITY OF MANITOBA   (Canada)

Author keywords

CE; Electrophoretic mobility; Escherichia coli galactosidase; Microheterogeneity; Single molecule

Indexed keywords

CATALYST ACTIVITY; ELECTROPHORETIC MOBILITY; ENZYME ACTIVITY; MOLECULES;

CE; CONTINUOUS-FLOW ASSAYS; ELEVATED TEMPERATURE; ENZYME MOLECULES; ESCHERICHIA COLI Β-GALACTOSIDASE; MEASUREMENTS OF; MICROHETEROGENEITY; SIMULTANEOUS MEASUREMENT; SINGLE MOLECULE; Β- GALACTOSIDASE;

ESCHERICHIA COLI;

BACTERIAL ENZYME; BETA GALACTOSIDASE;

ARTICLE; ASSAY; CAPILLARY ELECTROPHORESIS; CATALYSIS; CONTROLLED STUDY; ELECTROPHORETIC MOBILITY; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME POLYMORPHISM; ESCHERICHIA COLI; INCUBATION TEMPERATURE;

EID: 59249087735     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200800482     Document Type: Article

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