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Volumn 323, Issue 5914, 2009, Pages 633-637

Ligand-dependent equilibrium fluctuations of single calmodulin molecules

Author keywords

[No Author keywords available]

Indexed keywords

CALMODULIN;

EID: 59149096062     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.1166191     Document Type: Article
Times cited : (186)

References (32)
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    • Materials and methods are available as supporting material on Science Online.
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    • The low instrumental drift now allows a force resolution of AFM that comes close to optical traps. In our experience, AFM offers a better length resolution, whereas optical tweezers, due to the much softer spring constants, have advantages if constant force conditions are required
    • The low instrumental drift now allows a force resolution of AFM that comes close to optical traps. In our experience, AFM offers a better length resolution, whereas optical tweezers, due to the much softer spring constants, have advantages if constant force conditions are required.
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    • The expected contour length increase upon unfolding of a protein domain consisting of N amino acid residues can be calculated as ΔL, 0.365N, dfolded(I, dfolded(II, where dfolded(I) and dfolded(II) are the end-to-end distances of the folded structure before and after the unfolding event, respectively SOM text, Due to the a-helical linker between DomN and DomC, the domain boundaries of CaM are not precisely defined, which may account for the small deviations from the expected values
    • folded(II) are the end-to-end distances of the folded structure before and after the unfolding event, respectively (SOM text). Due to the a-helical linker between DomN and DomC, the domain boundaries of CaM are not precisely defined, which may account for the small deviations from the expected values.
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    • A simple thermodynamic argument can readily explain why a 1:1 stoichiometry should lead to concentration independent forces and transition rates (dashed lines in Fig. 3B). In our assay, the fully folded CaM is transformed to CaM with unfolded DomN in the first unfolding peak. Because at all Mas concentrations used (>0.3 mM), both intact CaM and DomC alone are fully saturated with Mas, changes in concentration cannot affect the energetics of this transition any further (SOM text).
    • A simple thermodynamic argument can readily explain why a 1:1 stoichiometry should lead to concentration independent forces and transition rates (dashed lines in Fig. 3B). In our assay, the fully folded CaM is transformed to CaM with unfolded DomN in the first unfolding peak. Because at all Mas concentrations used (>0.3 mM), both intact CaM and DomC alone are fully saturated with Mas, changes in concentration cannot affect the energetics of this transition any further (SOM text).
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    • We thank P. M. Bayley for inspiring discussions and A. Bausch, H. Gaub, M. Bertz, and M. Schlierf for helpful comments on the manuscript. This work was supported by Deutsche Forschungsgemeinschaft grant RI 990/3-1. J.P.J. was supported by the International Graduate School Materials Science of Complex Interfaces.
    • We thank P. M. Bayley for inspiring discussions and A. Bausch, H. Gaub, M. Bertz, and M. Schlierf for helpful comments on the manuscript. This work was supported by Deutsche Forschungsgemeinschaft grant RI 990/3-1. J.P.J. was supported by the International Graduate School "Materials Science of Complex Interfaces."


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.