Behavior of mesenchymal stem cells in the chemical microenvironment of the intervertebral disc

Spine

Volumn 33, Issue 17, 2008, Pages 1843-1849

  Wuertz, Karin ^a   Godburn, Karolyn ^a   Neidlinger Wilke, Cornelia ^b   Urban, Jocelyn ^c   Iatridis, James C ^a  

^a University of Vermont   (United States)

^b UNIVERSITY OF ULM   (Germany)

^c UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Glucose; Intervertebral disc; Mesenchymal stem cells; Microenvironment; Osmolarity; pH

Indexed keywords

AGGRECAN; COLLAGEN TYPE 1; GLUCOSE; MATRIX PROTEIN;

ACIDITY; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BEHAVIOR; BIOSYNTHESIS; BONE MARROW; CELL ISOLATION; CELL PROLIFERATION; CELL VIABILITY; CHEMICAL ENVIRONMENT; CULTURE MEDIUM; FLUORESCENCE; GENE EXPRESSION; INTERVERTEBRAL DISK; MESENCHYMAL STEM CELL; MONOLAYER CULTURE; NITROBLUE TETRAZOLIUM TEST; NONHUMAN; NUCLEUS PULPOSUS; OSMOLARITY; PH; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAINING; TISSUE REPAIR; ANIMAL; CELL CULTURE; CELL SURVIVAL; CHEMISTRY; COMPARATIVE STUDY; CYTOLOGY; ENVIRONMENT; NEWBORN; PHYSIOLOGY;

ANIMALS; ANIMALS, NEWBORN; CELL PROLIFERATION; CELL SURVIVAL; CELLS, CULTURED; ENVIRONMENT; INTERVERTEBRAL DISK; MESENCHYMAL STEM CELLS; RATS;

EID: 58949103805     PISSN: 03622436     EISSN: 15281159     Source Type: Journal    
DOI: 10.1097/BRS.0b013e31817b8f53     Document Type: Article

References (40)

1. Andersson GB. Epidemiological features of chronic low-back pain. Lancet 1999;3 54:581-5.
2. Katz JN. Lumbar disc disorders and low-back pain: socioeconomic factors and consequences. J Bone Joint Surg Am 2006;88(suppl 2):21-4.
3. Buck waiter JA. Aging and degeneration of the human intervertebral disc. Spine 1995;20:1307-14.
4. Holm S. Pathophysiology of disc degeneration. Acta Orthop Scand Suppl 1993;251:13-5.
5. Osti OL, Cullum DE. Occupational low back pain and intervertebral disc degeneration: epidemiology, imaging, and pathology. Clin J Pain 1994;10: 331-4.
6. Yoon ST, Patel NM. Molecular therapy of the intervertebral disc. Eur Spine J 2006;15(suppl3):S379-88.
7. Masuda K, An HS. Prevention of disc degeneration with growth factors. Eur Spine J 2006;15(snppl 3):S422-32.
8. Javazon EH, Colter DC, Schwarz EJ, et al. Rat marrow stromal cells are more sensitive to plating density and expand more rapidly from single-cell-derived colonies than human marrow stromal cells. Stem Cells 2001;19:219-25.
9. Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science1999;284:143-7.
10. Moore KA, Lemischka IR. Stem cells and their niches. Science2006;311: 1880-5.
11. Bibby SR, Jones DA, Ripley RM, et al. Metabolism of the intervertebral disc: effects of low levels of oxygen, glucose, and pH on rates of energy metabolism of bovine nucleus pulposus cells. Spine 2005;30:487-96.
12. Bibby SR, Urban JP. Effect of nutrient deprivation on the viability of intervertebral disc cells. Eur Spine J 2004;13-.695-701.
13. Grunhagen T, Wilde G, Soukane DM, et al. Nutrient supply and intervertebral disc metabolism. J Bone Joint Surg Am 2006;88(suppl 2):30-5.
14. Haschtmann D, Stoyanov JV, Ferguson SJ. Influence of diurnal hyperos-motic loading on the metabolism and matrix gene expression of a whole-organ intervertebral disc model. J Orthop Res 2006;,24:1957-66.
15. Holm S, Maroudas A, Urban JP, et al. Nutrition of the intervertebral disc: solute transport and metabolism. Connect Tissue Res 1981;8:101-19.
16. Ichimura K, Tsuji H, Matsui H, et al. Cell culture of the intervertebral disc of rats: factors influencing culture, proteoglycan, collagen, and deoxyribonu-cleic acid synthesis. J Spinal Disord 1991;4:428-36.
17. Ohshima H, Urban JP. The effect of lactate and pH on proteoglycan and protein synthesis rates in the intervertebral disc. Spine 1992;17:1079-82.
18. Razaq S, Wilkins RJ, Urban JP. The effect of extracellular pH on matrix turnover by cells of the bovine nucleus pulposus. Eur Spine J2003 ;12:341-9.
19. Urban JP. The role of the physicochemical environment in determining disc cell behaviour. Biochem Soc Trans 2002;30:858-64.
20. Wuertz K, Urban JP, Klasen J, et al. Influence of extracellular osmolarity and mechanical stimulation on gene expression of intervertebral disc cells. J Orthop Res 2007;25:1513-22.
21. Eggum TJ, Hunter CJ. "Educated" cells for IVD therapy: differential re- sponse of MSC to oxygen, glucose, and notochord conditioned medium. Trans Orthop Res Soc, 2006.
22. Stolzing A, Coleman N, Scutt A. Glucose-induced replicative senescence in mesenchymal stem cells. Rejuvenation Res 2006;9:31-5.
23. Schafer R, Knauf U, Zweyer M, et al. Age dependence of the human skeletal muscle stem cell in forming muscle tissue. Artif Organs 2006;30:130-40.
24. Stolzing A, Scutt A. Age-related impairment of mesenchymal progenitor cell function. Aging Cell 2006;5:213-24.
25. Zheng H, Martin JA, Buckwalter JA. Age related effects on chondrogenic differentiation of rat bone marrow stromal cells. Meeting of the Orthopaedic Research Society Chicago, 2006.
26. Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibro-blast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet 1970;3:393-403.
27. Diamant B, Karlsson J, Nachemson A. Correlation between lactate levels and pH in discs of patients with lumbar rhizopathies. Experientia 1968;24: 1195-6.
28. Kitano T, Zerwekh JE, Usui Y, et al. Biochemical changes associated with the symptomatic human intervertebral disk. Clin Orthop Relat Res 1993:372-7.
29. Maclean JJ, Lee CR, Alini M, et al. Anabolic and catabolic mRNA levels of the intervertebral disc vary with the magnitude and frequency of in vivo dynamic compression. J Orthop Res 2004;22:1193-200.
30. MacLean JJ, Lee CR, Alini M, et al. The effects of short-term load duration on anabolic and catabolic gene expression in the rat tail intervertebral disc. J Orthop Res 2005;23:1120-7.
31. Marlovits S, Hombauer M, Truppe M, et al. Changes in the ratio of type-I and type-II collagen expression during monolayer culture of human chon-drocytes. J Bone Joint Surg Br 2004;86:286-95.
32. Uchiyama Y, Cheng CC, Danielson KG, et al. Expression of acid-sensing ion channel 3 (ASIC3) in nucleus pulposus cells of the intervertebral disc is regulated by p75NTR and ERK signaling. J Bone Miner Res 2007;22:1996-2006.
33. Wuertz K, Godburn K, Iatridis CJ. pH dose response of mesenchymal stem cells: investigation of a critical factor in intervertebral disc repair. Meeting of the Orthopaedic Research Society, San Francisco, 2008.
34. Leung VY, Chan D, Cheung KM. Regeneration of intervertebral disc by mesenchymal stem cells: potentials, limitations, and future direction. Eur Spine J 2006 ;15(suppl 15):406-13.
35. Wang JY, Baer AE, Kraus VB, et al. Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture. Spine 2001;26: 1747-51; discussion 52.
36. Horner HA, Roberts S, Bielby RC, et al. Cells from different regions of the intervertebral disc: effect of culture system on matrix expression and cell phenotype. Spine 2002;27:1018-28.
37. Melrose J, Smith S, Ghosh P, et al. Differential expression of proteoglycan epitopes and growth characteristics of intervertebral disc cells grown in alginate bead culture. Cells Tissues Organs 2001;168:137-46.
38. Agrawal A, Guttapalli A, Narayan S, et al. Normoxic stabilization of HIF-lalpha drives glycolytic metabolism and regulates aggrecan gene expression in nucleus pulposus cells of the rat intervertebral disk. Am J Physiol Cell Physiol 2007;293:C621-31.
39. Risbud MV, Guttapalli A, Stokes DG, et al. Nucleus pulposus cells express HIF-lalpha under normoxic culture conditions: a metabolic adaptation to the intervertebral disc microenvironment. J Cell Biochem 2006;98:152-9.
40. Steck E, Bertram H, Abel R, et al. Induction of intervertebral disc-like cells from adult mesenchymal stem cells. Stem Cells 2005;23:403-11.