The effects of short-term load duration on anabolic and catabolic gene expression in the rat tail intervertebral disc

Journal of Orthopaedic Research

Volumn 23, Issue 5, 2005, Pages 1120-1127

  MacLean, Jeffery J ^a,b   Lee, Cynthia R ^a,b   Alini, Mauro ^b,c   Iatridis, James C ^a  

^a University of Vermont   (United States)

^b AO RESEARCH INSTITUTE   (Switzerland)

^c MCGILL UNIVERSITY   (Canada)

Author keywords

Dynamic compression; In vivo; Mechanobiology; Rat tail model; Real time RT PCR

Indexed keywords

AGGRECAN; COLLAGEN TYPE 1; COLLAGEN TYPE 2; COLLAGENASE 3; MESSENGER RNA; STROMELYSIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BIOMECHANICS; BONE STRESS; COMPRESSION; COMPRESSIVE STRENGTH; CONTROLLED STUDY; GENE EXPRESSION; INTERVERTEBRAL DISK; MECHANICAL STRESS; NONHUMAN; NUCLEUS PULPOSUS; PRIORITY JOURNAL; RAT; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STATISTICAL ANALYSIS; STATISTICAL SIGNIFICANCE; WEIGHT BEARING;

ADAM PROTEINS; AGGRECANS; ANIMALS; COLLAGEN TYPE II; COLLAGENASES; EXTRACELLULAR MATRIX PROTEINS; INTERVERTEBRAL DISK; LECTINS, C-TYPE; MATRIX METALLOPROTEINASE 13; MATRIX METALLOPROTEINASE 3; METALLOENDOPEPTIDASES; POLYMERASE CHAIN REACTION; PROCOLLAGEN N-ENDOPEPTIDASE; PROTEOGLYCANS; RATS; RATS, WISTAR; RNA, MESSENGER; STRESS, MECHANICAL; TAIL; TIME FACTORS;

EID: 24344449983     PISSN: 07360266     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.orthres.2005.01.020     Document Type: Article

References (42)

1. Applied-Biosystems: User Bulletin #2 for ABI Prism 7700 Sequence Detection System. 2001
2. M.T. Bayliss J.P. Urban B. Johnstone S. Holm In vitro method for measuring synthesis rates in the intervertebral disc J Orthop Res 4 1986 10-17
3. P. Brinckmann W. Frobin E. Hierholzer M. Horst Deformation of the vertebral end-plate under axial loading of the spine Spine 8 1983 851-856
4. J.T. Chang I.H. Chen C.T. Liao H.M. Wang Y.M. Hsu K.F. Hung et al. A reverse transcription comparative real-time PCR method for quantitative detection of angiogenic growth factors in head and neck cancer patients Clin Biochem 35 2002 591-596
5. J. Chen A.E. Baer P.Y. Paik W. Yan L.A. Setton Matrix protein gene expression in intervertebral disc cells subjected to altered osmolarity Biochem Biophys Res Commun 293 2002 932-938
6. C.T. Ching D.H. Chow F.Y. Yao A.D. Holmes Changes in nuclear composition following cyclic compression of the intervertebral disc in an in vivo rat-tail model Med Eng Phys 26 2004 587-594
7. C.T. Ching D.H. Chow F.Y. Yao A.D. Holmes The effect of cyclic compression on the mechanical properties of the inter-vertebral disc: An in vivo study in a rat tail model Clin Biomech (Bristol, Avon) 18 2003 182-189
8. G. Cs-Szabo D. Ragasa-San Juan V. Turumella K. Masuda E.J. Thonar H.S. An Changes in mRNA and protein levels of proteoglycans of the anulus fibrosus and nucleus pulposus during intervertebral disc degeneration Spine 27 2002 2212-2219
9. D.M. Elliott J.J. Sarver Young investigator award winner: Validation of the mouse and rat disc as mechanical models of the human lumbar disc Spine 29 2004 713-722
10. P. Flecknell J.V. Roughan R. Stewart Use of oral buprenorphine ('buprenorphine jello') for postoperative analgesia in rats - a clinical trial Lab Anim 33 1999 169-174
11. T. Handa H. Ishihara H. Ohshima R. Osada H. Tsuji K. Obata Effects of hydrostatic pressure on matrix synthesis and matrix metalloproteinase production in the human lumbar intervertebral disc Spine 22 1997 1085-1091
12. H.A. Horner J.P. Urban 2001 Volvo Award Winner in Basic Science Studies: Effect of nutrient supply on the viability of cells from the nucleus pulposus of the intervertebral disc Spine 26 2001 2543-2549
13. A.H. Hsieh J.C. Lotz Prolonged spinal loading induces matrix metalloproteinase-2 activation in intervertebral discs Spine 28 2003 1781-1788
14. W.C. Hutton W.A. Elmer S.D. Boden S. Hyon Y. Toribatake K. Tomita et al. The effect of hydrostatic pressure on intervertebral disc metabolism Spine 24 1999 1507-1515
15. W.C. Hutton W.A. Elmer L.M. Bryce E.E. Kozlowska S.D. Boden M. Kozlowski Do the intervertebral disc cells respond to different levels of hydrostatic pressure? Clin Biomech (Bristol, Avon) 16 2001 728-734
16. W.C. Hutton T.M. Ganey W.A. Elmer E. Kozlowska J.L. Ugbo E.S. Doh et al. Does long-term compressive loading on the intervertebral disc cause degeneration? Spine 25 2000 2993-3004
17. W.C. Hutton Y. Toribatake W.A. Elmer T.M. Ganey K. Tomita T.E. Whitesides The effect of compressive force applied to the intervertebral disc in vivo. A study of proteoglycans and collagen Spine 23 1998 2524-2537
18. W.C. Hutton S.T. Yoon W.A. Elmer J. Li H. Murakami A. Minamide et al. Effect of tail suspension (or simulated weightlessness) on the lumbar intervertebral disc: Study of proteoglycans and collagen Spine 27 2002 1286-1290
19. J.C. Iatridis P.L. Mente I.A. Stokes D.D. Aronsson M. Alini Compression-induced changes in intervertebral disc properties in a rat tail model Spine 24 1999 996-1002
20. Iatridis JC, Risinger R, Monsey R, Goswanmi T, Alini M. Effects of static compression on biochemical composition of caudal discs in vivo. Trans International Soc Lumbar Spine (ISSLS) Vancouver: Canada; 2003, Poster 101
21. H. Ishihara D.S. McNally J.P. Urban A.C. Hall Effects of hydrostatic pressure on matrix synthesis in different regions of the intervertebral disk J Appl Physiol 80 1996 839-846
22. H. Ishihara J.P. Urban Effects of low oxygen concentrations and metabolic inhibitors on proteoglycan and protein synthesis rates in the intervertebral disc J Orthop Res 17 1999 829-835
23. H. Ishihara K. Warensjo S. Roberts J.P. Urban Proteoglycan synthesis in the intervertebral disk nucleus: The role of extracellular osmolality Am J Physiol 272 1997 C1499-C1506
24. W. Johannessen E.J. Vresilovic A.C. Wright D.M. Elliott Intervertebral disc mechanics are restored following cyclic loading and unloaded recovery Ann Biomed Eng 32 2004 70-76
25. M.R. Johnson K. Wang J.B. Smith M.J. Heslin R.B. Diasio Quantitation of dihydropyrimidine dehydrogenase expression by real-time reverse transcription polymerase chain reaction Anal Biochem 278 2000 175-184
26. M. Kasra V. Goel J. Martin S.T. Wang W. Choi J. Buckwalter Effect of dynamic hydrostatic pressure on rabbit intervertebral disc cells J Orthop Res 21 2003 597-603
27. C.H. Kim F.H. Chen M.L. Chan G.A. Ateshian C.T. Hung X.E. Guo Intervertebral disc response to in vivo dynamic loading in a rat-tail model Trans Orthop Res Soc 28 2003 Paper 57
28. M.D. Kinkel W.E. Horton Jr. Coordinate down-regulation of cartilage matrix gene expression in Bcl-2 deficient chondrocytes is associated with decreased SOX9 expression and decreased mRNA stability J Cell Biochem 88 2003 941-953
29. Lee CK, Grad S, MacLean JJ, Iatridis JC, Alini M. Effect of mechanical loading on mRNA levels of common endogenous controls in articular chondrocytes and intervertebral disc. Anal Biochem, in press
30. J.C. Lotz J.R. Chin Intervertebral disc cell death is dependent on the magnitude and duration of spinal loading Spine 25 2000 1477-1483
31. J.C. Lotz O.K. Colliou J.R. Chin N.A. Duncan E. Liebenberg Compression-induced degeneration of the intervertebral disc: An in vivo mouse model and finite-element study Spine 23 1998 2493-2506
32. J.J. MacLean C.R. Lee M. Alini J.C. Iatridis Anabolic and catabolic mRNA levels of the intervertebral disc vary with the magnitude and frequency of in vivo dynamic compression J Orthop Res 22 2004 1193-1200
33. J.J. MacLean C.R. Lee S. Grad K. Ito M. Alini J.C. Iatridis Effects of immobilization and dynamic compression on intervertebral disc cell gene expression in vivo Spine 28 2003 973-981
34. A.L. Nachemson Disc pressure measurements Spine 6 1981 93-97
35. H. Ohshima J.P. Urban The effect of lactate and pH on proteoglycan and protein synthesis rates in the intervertebral disc Spine 17 1992 1079-1082
36. H. Oshima H. Ishihara J.P. Urban H. Tsuji The use of coccygeal discs to study intervertebral disc metabolism J Orthop Res 11 1993 332-338
37. P.M. Ragan A.M. Badger M. Cook V.I. Chin M. Gowen A.J. Grodzinsky et al. Down-regulation of chondrocyte aggrecan and type-II collagen gene expression correlates with increases in static compression magnitude and duration J Orthop Res 17 1999 836-842
38. I.A. Stokes D.D. Aronsson H. Spence J.C. Iatridis Mechanical modulation of intervertebral disc thickness in growing rat tails J Spinal Disord 11 1998 261-265
39. Upton ML, Kazmierski R, Chen J, Guilak F, Setton LA. Cyclic tensile stretch increases gene expression in meniscal cells in a time dependent manner. In: Trans Orthop Res Soc. San Francisco, 2004, Poster No: 0815
40. J.P. Urban The role of the physicochemical environment in determining disc cell behaviour Biochem Soc Trans 30 2002 858-864
41. A.J. Walsh J.C. Lotz Biological response of the intervertebral disc to dynamic loading J Biomech 37 2004 329-337
42. H.J. Wilke P. Neef M. Caimi T. Hoogland L.E. Claes New in vivo measurements of pressures in the intervertebral disc in daily life Spine 24 1999 755-762