Isolation, expression and characterization of two single-chain variable fragment antibodies against an endo-polygalacturonase secreted by Sclerotinia sclerotiorum

Protein Expression and Purification

Volumn 64, Issue 2, 2009, Pages 237-243

  Yang, Bo ^a   Yajima, William ^a   Das, Dipankar ^a   Suresh, Mavanur R ^a   Kav, Nat N V ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Endo polygalacturonase; Phage display; Sclerotinia sclerotiorum; Single chain variable fragment; Surface plasmon resonance

Indexed keywords

FUNGUS ANTIBODY; PEPTIDE LIBRARY; POLYGALACTURONASE; RECOMBINANT PROTEIN;

ANIMAL; ANTIBODY SPECIFICITY; ARTICLE; ASCOMYCETES; ENZYMOLOGY; GENETICS; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOUSE; PLANT DISEASE; SURFACE PLASMON RESONANCE;

ANIMALS; ANTIBODIES, FUNGAL; ANTIBODY SPECIFICITY; ASCOMYCOTA; IMMUNOGLOBULIN VARIABLE REGION; MICE; PEPTIDE LIBRARY; PLANT DISEASES; POLYGALACTURONASE; RECOMBINANT PROTEINS; SURFACE PLASMON RESONANCE;

BRASSICA NAPUS; BRASSICA NAPUS VAR. NAPUS; FUNGI; MUS; SCLEROTINIA SCLEROTIORUM;

EID: 58749097406     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.12.007     Document Type: Article

References (41)

1. Boland G.J., and Hall R. Index of plant hosts of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology-Revue Canadienne De Phytopathologie 16 (1994) 93-108
2. Bolton M.D., Thomma B., and Nelson B.D. Sclerotinia sclerotiorum (Lib.) de Bary: biology and molecular traits of a cosmopolitan pathogen. Molecular Plant Pathology 7 (2006) 1-16
3. Bom M., and Boland G.J. Evaluation of disease forecasting variables for sclerotinia stem rot (Sclerotinia sclerotiorum) of canola. Canadian Journal of Plant Science 80 (2000) 889-898
4. Hambleton S., Walker C., and Kohn L.M. Clonal lineages of Sclerotinia sclerotiorum previously known from other crops predominate in 1999-2000 samples from Ontario and Quebec soybean. Canadian Journal of Plant Pathology 24 (2002) 309-315
5. Anonymous, Field Crop Reporting Series. Statistics Canada (2007) 13-14.
6. Thomas P. Sclerotinia stem rot checklist. Canola Growers Manual (1984), Canola Council of Canada, Winnipeg
7. Turkington T.K., and Morrall R.A.A. Use of petal infestation to forecast Sclerotinia stem rot of canola - the influence of inoculum variation over the flowering period and canopy density. Phytopathology 83 (1993) 682-689
8. Turkington T.K., Morrall R.A.A., and Gugel R.K. Use of petal infestation to forecast Sclerotinia stem rot of canola - evaluation of early bloom sampling, 1985-90. Canadian Journal of Plant Pathology-Revue Canadienne De Phytopathologie 13 (1991) 50-59
9. Turkington T.K., Morrall R.A.A., and Rude S.V. Use of petal infestation to forecast Sclerotinia stem rot of canola - the impact of diurnal and weather-related inoculum fluctuations. Canadian Journal of Plant Pathology-Revue Canadienne De Phytopathologie 13 (1991) 347-355
10. Bateman D.F., and Beer S.V. Simultaneous production and synergistic action of oxalic acid and polygalacturonase during pathogenesis by Sclerotinia sclerotiorum rolfsii. Phytopathology 55 (1965) 204-211
11. Cessna S.G., Sears V.E., Dickman M.B., and Low P.S. Oxalic acid, a pathogenicity factor for Sclerotinia sclerotiorum, suppresses the oxidative burst of the host plant. Plant Cell 12 (2000) 2191-2200
12. Godoy G., Steadman J.R., Dickman M.B., and Dam R. Use of mutants to demonstrate the role of oxalic-acid in pathogenicity of Sclerotinia sclerotiorum on Phaseolus vulgaris. Physiological and Molecular Plant Pathology 37 (1990) 179-191
13. Guimaraes R.L., and Stotz H.U. Oxalate production by Sclerotinia sclerotiorum deregulates guard cells during infection. Plant Physiology 136 (2004) 3703-3711
14. Kim K.S., Min J.Y., and Dickman M.B. Oxalic acid is an elicitor of plant programmed cell death during Sclerotinia sclerotiorum disease development. Molecular Plant-Microbe Interactions 21 (2008) 605-612
15. Kolkman J.M., and Kelly J.D. An indirect test using oxalate to determine physiological resistance to white mold in common bean. Crop Science 40 (2000) 281-285
16. Noyes R.D., and Hancock J.G. Role of oxalic acid in the Sclerotinia sclerotiorum wilt of sunflower Helianthus annuus. Physiological and Molecular Plant Pathology 18 (1981) 123-132
17. Kasza Z., Vagvölgyi C., Févre M., and Cotton P. Molecular characterization and in planta detection of Sclerotinia sclerotiorum endopolygalacturonase genes. Current Microbiology 48 (2004) 208-213
18. Li R.G., Rimmer R., Buchwaldt L., Sharpe A.G., Seguin-Swartz G., Coutu C., and Hegedus D.D. Interaction of Sclerotinia sclerotiorum with a resistant Brassica napus cultivar: expressed sequence tag analysis identifies genes associated with fungal pathogenesis. Fungal Genetics and Biology 41 (2004) 735-753
19. Li R.G., Rimmer R., Buchwaldt L., Sharpe A.G., Seguin-Swartz G., and Hegedus D.D. Interaction of Selerotinia sclerotiorum with Brassica napus: cloning and characterization of endo- and exo-polygalacturonases expressed during saprophytic and parasitic modes. Fungal Genetics and Biology 41 (2004) 754-765
20. Yajima W., and Kav N.N.V. The proteorne of the phytopathogenic fungus Sclerotinia sclerotiorum. Proteomics 6 (2006) 5995-6007
21. Erental A., Harel A., and Yarden O. Type 2A phosphoprotein phosphatase is required for asexual development and pathogenesis of Sclerotinia sclerotiorum. Molecular Plant-Microbe Interactions 20 (2007) 944-954
22. Harel A., Bercovich S., and Yarden O. Calcineurin is required for sclerotial development and pathogenicity of Sclerotinia sclerotiorum in an oxalic acid-independent manner. Molecular Plant-Microbe Interactions 19 (2006) 682-693
23. Rollins J.A. The Sclerotinia sclerotiorum pac1 gene is required for sclerotial development and virulence. Molecular Plant-Microbe Interactions 16 (2003) 785-795
24. Vega R.R., Corsini D., and Letourne D. Nonvolatile organic acids Produced by Sclerotinia Sclerotiorum in synthetic liquid media. Mycologia 62 (1970) 332-338
25. De Lorenzo G., D'Ovidio R., and Cervone F. The role of polygalacturonase-inhibiting proteins (PGIPs) in defense against pathogenic fungi. Annual Review of Phytopathology 39 (2001) 313-335
26. Zuppini A., Navazio L., Sella L., Castiglioni J., Favaron F., and Mariani P. An endopolygalacturonase from Sclerotinia sclerotiorum induces calcium-mediated signaling and programmed cell death in soybean cells. Molecular Plant-Microbe Interactions 18 (2005) 849-855
27. Rollins J.A., and Dickman M.B. PH signaling in Sclerotinia sclerotiorum: identification of a pacC/RIM1 Homolog. Applied and Environmental Microbiology 67 (2001) 75-81
28. Fraissinet-Tachet L., Reymond-Cotton R., and Fevre M. Characterization of a multigene family encoding an endopolygalacturonase in Sclerotinia sclerotiorum. Current Genetics 29 (1995) 96-100
29. Yajima W., Rahman M.H., Das D., Suresh M.R., and Kav N.N. Detection of Sclerotinia sclerotiorum using a monomeric and dimeric single-chain fragment variable (scFv) antibody. Journal of Agricultural and Food Chemistry 56 (2008) 9455-9463
30. J. Burmester, A. Plcükthun, Im: Antibody Engineering. R. Kontermann and S. Dübel, Eds. (2001) 19-40.
31. Krebber A., Bornhauser S., Burmester J., Honegger A., Willuda J., Bosshard H.R., and Pluckthun A. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system. Journal of Immunological Methods 201 (1997) 35-55
32. Tout N.L., Yau K.Y.F., Trevors J.T., Lee H., and Hall J.C. Synthesis of ligand-specific phage-display scFv against the herbicide picloram by direct cloning from hyperimmunized mouse. Journal of Agricultural and Food Chemistry 49 (2001) 3628-3637
33. Das D., Kriangkum J., Nagata L.P., Fulton R.E., and Suresh M.R. Development of a biotin mimic tagged ScFv antibody against western equine encephalitis virus: bacterial expression and refolding. Journal of Virological Methods 117 (2004) 169-177
34. Delos S.E., Gilbert J.M., and White J.M. The central proline of an internal viral fusion peptide serves two important roles. Journal of Virology 74 (2000) 1686-1693
35. Corr M., Slanetz A.E., Boyd L.F., Jelonek M.T., Khilko S., Alramadi B.K., Kim Y.S., Maher S.E., Bothwell A.L.M., and Margulies D.H. T-Cell Receptor-Mhc Class-I Peptide Interactions - Affinity, Kinetics, and Specificity. Science 265 (1994) 946-949
36. Schouten A., Roosien J., deBoer J.M., Wilmink A., Rosso M.N., Bosch D., Stiekema W.J., Gommers F.J., Bakker J., and Schots A. Improving scFv antibody expression levels in the plant cytosol. FEBS Letters 415 (1997) 235-241
37. Altschuh D., Dubs M., Weiss E., Zeder-Lutz G., and Van Regenmortel M. Determination of kinetic constants for the interaction between a monoclonal antibody and peptides using surface plasmon resonance. Biochemistry 31 (1992) 6298-6304
38. Choulier L., Laune D., Orfanoudakis G., Wlad H., Janson J., Granier C., and Altschuh D. Delineation of a linear epitope by multiple peptide synthesis and phage display. Journal of Immunological Methods 249 (2001) 253-264
39. Huang B.C., Davern S., and Kennel S.J. Mono and bivalent binding of a scFv and covalent diabody to murine laminin-1 using radioiodinated proteins and SPR measurements: Effects on tissue retention in vivo. Journal of Immunological Methods 313 (2006) 149-160
40. Leonard P., Safsten P., Hearty S., McDonnell B., Finlay W., and O'Kennedy R. High throughput ranking of recombinant avian scFv antibody fragments from crude lysates using the Biacore A100. Journal of Immunological Methods 323 (2007) 172-179
41. Reiter Y., Schuck P., Boyd L.F., and Plaksin D. An antibody single-domain phage display library of a native heavy chain variable region: isolation of functional single-domain VH molecules with a unique interface. Journal of Molecular Biology 290 (1999) 685-698