Effector caspase activation, in the absence of a conspicuous apoptosis induction, in mononuclear cells treated with azidothymidine

Pharmacological Research

Volumn 59, Issue 2, 2009, Pages 125-133

  Matteucci, Claudia ^a   Minutolo, Antonella ^a   Balestrieri, Emanuela ^a   Ascolani, Arianna ^a   Grelli, Sandro ^a   Macchi, Beatrice ^a,b   Mastino, Antonio ^c,d  

^a UNIVERSITY OF ROME TOR VERGATA   (Italy)

^b IRCCS FONDAZIONE SANTA LUCIA   (Italy)

^c Section of Microbiological   (Italy)

^d IRCCS CENTRO NEUROLESI BONINO PULEJO   (Italy)

Author keywords

Apoptosis; Azidothymidine; Caspases

Indexed keywords

BETA ACTIN; CASPASE; CASPASE 3; CASPASE 7; CASPASE 8; CASPASE 9; FAS ANTIGEN; FLUOROURACIL; INHIBITOR OF APOPTOSIS PROTEIN; PROTEIN BCL 2; PROTEIN P53; ZIDOVUDINE;

APOPTOSIS; ARTICLE; CONTROLLED STUDY; DRUG CYTOTOXICITY; EFFECTOR CELL; ENZYME ACTIVATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IN VITRO STUDY; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION;

ANTI-HIV AGENTS; APOPTOSIS; CASPASES; CELLS, CULTURED; DNA DAMAGE; ENZYME ACTIVATION; HUMANS; LEUKOCYTES, MONONUCLEAR; ZIDOVUDINE;

EID: 58749083342     PISSN: 10436618     EISSN: 10961186     Source Type: Journal    
DOI: 10.1016/j.phrs.2008.11.003     Document Type: Article

References (26)

1. Chandrasekaran B., Kute T.E., and Duch D.S. Synchronization of cells in the S phase of the cell cycle by 3′-azido-3′-deoxythymidine: implications of cell cytotoxicity. Cancer Chemother Pharmacol 35 (1995) 489-495
2. Viora M., Di Genova G., Rivabene R., Malorni W., and Fattorossi A. Interference with cell cycle progression and induction of apoptosis by dideoxynucleoside analogs. Int J Immunopharmacol 19 (1997) 311-321
3. Olivero O.A. Mechanisms of genotoxicity of nucleoside reverse transcriptase inhibitors. Environ Mol Mutagen 48 (2007) 215-223
4. Sussman H.E., Olivero O.A., Meng Q., Pietras S.M., Poirier M.C., O'Neill J.P., et al. Genotoxicity of 3′-azido-3′-deoxythymidine in the human lymphoblastoid cell line, TK6: relationships between DNA incorporation, mutant frequency, and spectrum of deletion mutations in HPRT. Mutat Res 429 (1999) 249-259
5. Escobar P.A., Olivero O.A., Wade N.A., Abrams E.J., Nesel C.J., Ness R.B., et al. Genotoxicity assessed by the comet and GPA assays following in vitro exposure of human lymphoblastoid cells (H9) or perinatal exposure of mother-child pairs to AZT or AZT-3TC. Environ Mol Mutagen 48 (2007) 330-343
6. Roos W.P., and Kaina B. DNA damage-induced cell death by apoptosis. Trends Mol Med 12 (2006) 440-450
7. Sailaja G., Nayak R., and Antony A. Azidothymidine induces apoptosis in mouse myeloma cell line Sp2/0. Biochem Pharmacol 52 (1996) 857-862
8. Collier A.C., Helliwell R.J.A., Keelan J.A., Paxton J.W., Mitchell M.D., and Tingle M.D. 3′-Azido-3′-deoxythymidine (AZT) induces apoptosis and alters metabolic enzyme activity in human placenta. Toxicol Appl Pharmacol 192 (2003) 164-173
9. Benveniste O., Estaquier J., Lelievre J.D., Vilde J.L., Ameisen J.C., and Leport C. Possible mechanism of toxicity of zidovudine by induction of apoptosis of CD4+ and CD8+ T-cells in vivo. Eur J Clin Microbiol Infect Dis 20 (2001) 896-897
10. Matarrese P., Gambardella L., Cassone A., Vella S., Cauda R., and Malorni W. Mitochondrial membrane hyperpolarization hijacks activated T lymphocytes toward the apoptotic-prone phenotype: homeostatic mechanisms of HIV protease inhibitors. J Immunol 170 (2003) 6006-6015
11. Copper C.L., Phenix B., Mbisa G., Lum J.J., Parato K., Hawley N., et al. Antiretroviral therapy influences cellular susceptibility to apoptosis in vivo. Front Biosci 9 (2004) 338-341
12. Bazarbachi A., Nasr R., El-Sabban M.E., Mahé A., Mahieux R., Gessain A., et al. Evidence against a direct cytotoxic effect of alpha interferon and zidovudine in HTLV-I associated adult T cell leukemia/lymphoma. Leukemia 14 (2000) 716-721
13. Jiang B., Hebert V.Y., Li Y., Mathis J.M., Alexander J.S., and Dugas T.R. HIV antiretroviral drug combination induces endothelial mitochondrial dysfunction and reactive oxygen species production, but not apoptosis. Toxicol Appl Pharmacol 224 (2007) 60-71
14. Matteucci C., Grelli S., De Smaele E., Fontana C., and Mastino A. Identification of nuclei from apoptotic, necrotic, and viable lymphoid cells by using multiparameter flow cytometry. Cytometry 35 (1999) 145-153
15. Gao W.Y., Shirasaka T., Johns D.G., Broder S., and Mitsuya H. Differential phosphorylation of azidothymidine, dideoxycytidine, and dideoxyinosine in resting and activated peripheral blood mononuclear cells. J Clin Invest 91 (1993) 2326-2333
16. Boatrigh K.M., and Salvesen G.S. Caspase activation. Biochem Soc Symp 70 (2003) 233-242
17. Ji H.J., Rha S.Y., Jeung H.C., Yang S.H., An S.W., and Chung H.C. Cyclic induction of senescence with intermittent AZT treatment accelerates both apoptosis and telomere loss. Breast Cancer Res Treat 93 (2005) 227-236
18. Sato T., Neschadim A., Konrad M., Fowler D.H., Lavie A., and Medin J.A. Engineered human tmpk/AZT as a novel enzyme/prodrug axis for suicide gene therapy. Mol Ther 15 (2007) 962-970
19. Amè J.C., Spenlehauer C., and de Murcia G. The PARP superfamily. BioEssay 26 (2004) 882-893
20. Mortusewicz O., Ame J.C., Schreiber V., and Leonhardt H. Feedback-regulated poly(ADP-ribosyl)ation by PARP-1 is required for rapid response to DNA damage in living cells. Nucleic Acids Res 35 (2007) 7665-7675
21. Koh D.W., Lawler A.M., Poitras M.F., Sasaki M., Wattler S., Nehls M.C., et al. Failure to degrade poly(ADP-ribose) causes increased sensitivity to cytotoxicity and early embryonic lethality. Proc Natl Acad Sci USA 101 (2004) 17699-17704
22. Albeck J.G., Burke J.M., Aldridge B.B., Zhang M., Lauffenburger D.A., and Sorger P.K. Quantitative analysis of pathways controlling extrinsic apoptosis in single cells. Mol Cell 30 (2008) 11-25
23. Sheridan C., and Martin S.J. Commitment in apoptosis: slightly dead but mostly alive. Trends Cell Biol 18 (2008) 353-357
24. Lee R.K., Cai J.P., Deyev V., Gill P.S., Cabral L., Wood C., et al. Azidothymidine and interferon-alpha induce apoptosis in herpesvirus-associated lymphomas. Cancer Res 59 (1999) 5514-5520
25. Cinatl Jr. J., Kotchetkov R., Gröschel B., Cinatl J., Driever P.H., Kabickova H., et al. Azidothymidine resistance of H9 human T-cell lymphoma cells is associated with decreased sensitivity to antitumor agents and inhibition of apoptosis. Int J Mol Med 2 (1998) 685-691
26. Walker D.M., Malarkey D.E., Seilkop S.K., Ruecker F.A., Funk K.A., Wolfe M.J., et al. Trasplacental carcinogenicity of 3′-azido-3′deoxythymidine in B6C3F1 mice and F344 rats. Environ Mol Mutagen 48 (2007) 283-298