Molecular detection of Puccinia horiana in Chrysanthemum x morifolium through conventional and real-time PCR

Journal of Microbiological Methods

Volumn 76, Issue 2, 2009, Pages 136-145

  Alaei, Hossein ^a,b   Baeyen, Steve ^a   Maes, Martine ^a   Höfte, Monica ^b   Heungens, Kurt ^a  

^a INSTITUTE FOR AGRICULTURAL AND FISHERIES RESEARCH ILVO   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

Author keywords

Chrysanthemum white rust; Detection limit; DNA extraction; PCR inhibitors; Sensitivity; Specificity

Indexed keywords

GENOMIC DNA; INTERNAL TRANSCRIBED SPACER; INTERNAL TRANSCRIBED SPACER 1; INTERNAL TRANSCRIBED SPACER 2; PLANT DNA; RIBOSOME DNA;

ANIMAL EXPERIMENT; ARTICLE; CHRYSANTHEMUM; CHRYSANTHEMUM X MORIFOLIUM; CLONE; CONTROLLED STUDY; DNA EXTRACTION; FUNGAL DETECTION; FUNGUS; GENE AMPLIFICATION; GENE NUMBER; GENE TARGETING; INOCULATION; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PLANT DISEASE; PLANT TISSUE; PRIORITY JOURNAL; PUCCINIA HORIANA; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SIGNAL DETECTION;

BASIDIOMYCOTA; CETRIMONIUM COMPOUNDS; CHRYSANTHEMUM; DETERGENTS; DNA PRIMERS; DNA, FUNGAL; DNA, PLANT; DNA, RIBOSOMAL SPACER; FLUORESCENT DYES; ORGANIC CHEMICALS; PLANT DISEASES; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SPORES, FUNGAL;

ALBUGINACEAE; CHRYSANTHEMUM; CHRYSANTHEMUM X MORIFOLIUM; PUCCINIA HORIANA;

EID: 58549088348     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2008.10.001     Document Type: Article

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