Permeabilization method for in-situ investigation of fungal conidia on surfaces

Letters in Applied Microbiology

Volumn 48, Issue 2, 2009, Pages 234-240

  Villa, F ^a   Cappitelli, F ^a   Principi, P ^a   Polo, A ^a   Sorlini, C ^a  

^a UNIVERSITY OF MILAN   (Italy)

Author keywords

DAPI staining; FISH; Fungal conidia; Permeabilization; Spatial arrangement

Indexed keywords

ASPERGILLUS; FISH; OSMOSIS;

4′-6-DIAMIDINO-2-PHENYLINDOLE; COLONISATION; DIAMIDINO-2-PHENYLINDOLE STAINING; FLUORESCENT IN SITU HYBRIDIZATION; FUNGAL CONIDIUM; FUNGAL SPECIES; IN-SITU INVESTIGATIONS; PERMEABILIZATION; SPATIAL ARRANGEMENTS; WALL STRUCTURE;

SURFACE ACTIVE AGENTS;

4',6 DIAMIDINO 2 PHENYLINDOLE; ALCOHOL DERIVATIVE; EDETIC ACID; MERCAPTOETHANOL; SURFACTANT; TRITON X 100;

ALCOHOLS; BIODIVERSITY; DEHYDRATION; FLUORESCENCE; FUNGI; OSMOSIS;

ALCOHOL; DEHYDRATION; FUNGUS; GERM CELL; HYBRIDIZATION; OSMOREGULATION; SURFACTANT;

ACREMONIUM; ALTERNARIA; ARTICLE; ASPERGILLUS; ASPERGILLUS NIGER; CHAETOMIUM; CLADOSPORIUM; CONIDIUM; CONTROLLED STUDY; EMERICELLA; FLUORESCENCE IN SITU HYBRIDIZATION; FUNGAL STRAIN; FUSARIUM OXYSPORUM; HYPEROSMOTIC STRESS; INTERMETHOD COMPARISON; NONHUMAN; OSMOTIC STRESS; PENICILLIUM; PERMEABILITY; STAINING; SURFACE PROPERTY; TRICHODERMA;

ASPERGILLUS NIGER;

EID: 58449086071     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2008.02520.x     Document Type: Article

References (38)

1. Amann, R.I., Binder, R.J., Olson, S.W., Chisholm, R., Devereux, R. Stahl, D.A. (1990) Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial population. Appl Environ Microbiol 56, 1919 1925.
2. Amman, R Ludwing, W. (2000) Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology. FEMS Microbiol Rev 24, 555 565.
3. Aoi, Y. (2002) In situ identification of microorganisms in biofilm communities. J Biosci Bioeng 6, 552 556.
4. Barlocher, F. (2007) Molecular approaches applied to aquatic hyphomycetes. Fungal Biol Rev 21, 19 24.
5. Bottari, B., Ercolini, D., Gatti, M. Neviani, E. (2006) Application of FISH technology for microbiological analyisis: current state and prospects. Appl Microbiol Biotechnol 73, 485 494.
6. Cappitelli, F., Nosanchuk, J.D., Casadevall, A., Toniolo, L., Brusetti, L., Florio, S., Principi, P., Borin, S. et al. (2007) Synthetic consolidants attacked by melanin-producing fungi: case study of the biodeterioration on Milan (Italy) cathedral marble treated with acrylics. Appl Environ Microbiol 73, 271 277.
7. Cheatko, G. Bald, E. (2000) Determination of cysteine in human plasma by high-performance liquid chromatography and ultraviolet detection after pre-column derivatization with 2-chloro-1-methylpyridinium iodide. Talanta 5, 509 515.
8. Chen, R.R. (2007) Permeability issue in whole-cell bioprocesses and cellular membrane engineering. Appl Microbiol Biotechnol 74, 730 738.
9. Douglas, L.J. (2003) Candida biofilms and their role in infection. Trends Microbiol 11, 30 36.
10. Gorbushina, A.A. (2007) Life on the rocks. Environ Microbiol 9, 1613 1631.
11. Guillemot, G., Vaca-Medina, G., Martin-Yken, H., Vernhet, A., Schmitz, P. Mercier-Bonin, M. (2006) Shear-flow induced detachment of Saccharomyces cerevisiae from stainless steel: influence of yeast and solid surface properties. Colloid Surf B 49, 126 135.
12. Hayden, R.T., Isotalo, P.A., Parret, T., Wolk, D.M., Qian, X., Roberts, G.D. Lloyd, R.V. (2003) In situ hybridization of the differentiation of Aspergillus, Fusarium, and Pseudallescheria species in tissue section. Diagn Mol Pathol 12, 21 26.
13. Inàcio, J., Behrens, S., Fuchs, B.M., Fonseca, A., Spencer-Martins, I. Amann, R. (2003) In situ accessibility of Saccharomyces cerevisiae 26S rRNA to Cy3-labeled oligonucleotide probes comprising the D1 and D2 domains. Appl Environ Microbiol 69, 2899 2905.
14. Kempf, V., Mandle, T., Schumacher, U., Schafer, A. Autenrieth, I.B. (2005) Rapid detection and identification of pathogens in blood cultures by fluorescence in situ hybridization and flow cytometry. Int J Med Microbiol 295, 47 55.
15. Linder, M.B., Szilvay, G.R., Nakari-Setala, T. Penttila, M.E. (2005) Hydrophobins: the protein-amphiphiles of filamentous fungi. FEMS Microbiol Rev 29, 877 896.
16. Manz, W., Amann, R., Ludwig, W., Wagner, M. Schleifer, K.H. (1992) Phylogenetic oligonucleotide probes for the major subclasses of proteobacteria: problems and solutions. Syst Appl Microbiol 15, 593 600.
17. Mille, Y., Beney, L. Gervais, P. (2005) Compared tolerance to osmotic stress in various microorganisms: towards a survival prediction test. Biotechnol Bioeng 92, 479 484.
18. Norrish, R.S. (1966) An equation for the activity coefficients and equilibrium relative humidities of water in confectionery syrups. J Food Technol 1, 25 39.
19. Oulahal-Lagsir, N., Martial-Gros, A., Boistier, E., Blum, L.J. Bonneau, M. (2000) The development of an ultrasonic apparatus for the non-invasive and repeatable removal of fouling food processing equipment. Lett Appl Microbiol 30, 47 52.
20. Paris, S., Debeaupuis, J.P., Crameri, R., Carey, M., Charles, F., Prévost, M.C., Schmitt, C., Philippe, B. et al. (2003) Conidial hydrophobins of Aspergillus fumigatus. Appl Environ Microbiol 69, 1581 1588.
21. Pawlowska, T.E. (2005) Genetic processes in arbuscolar mycorrhizal fungi. FEMS Microbiol Lett 251, 185 191.
22. Prigione, V. Marchisio, V.F. (2004) Methods to maximise the staining of fungal propagules with fluorescent dyes. J Microbiol Meth 59, 371 379.
23. Prigione, V., Lingua, G. Filipello Marchisio, V. (2004) Development and use of flow cytometry for detection of airborne fungi. Appl Environ Microbiol 70, 1360 1365.
24. Ramachandran, S., Fontanille, P., Pandey, A. Larroche, C. (2007) Spores of Aspergillus niger as reservoir of glucose oxidase synthesized during solid-state fermentation and their use as catalyst in gluconic acid production. Lett Appl Microbiol 44, 155 160.
25. Rigby, S., Procop, G.W., Haase, G., Wilson, D., Hall, G., Kurtzman, C., Oliveira, K., Von Oy, S. et al. (2002) Fluorescence in situ hybridization with peptide nucleic acid probes for rapid identification of Candida albicans directly from blood culture bottles. J Clin Microbiol 40, 2182 2186.
26. Sarkany, I. Gaylarde, C.C. (1968) Bacterial colonisation of the skin flora of the newborn. J Pathol Bacteriol 95, 115 122.
27. Schuster, E., Dunn-Coleman, N., Frisvad, J.C. van Dijck, P.W.M. (2002) On the safety of Aspergillus niger- a review. Appl Microbiol Biotechnol 59, 426 435.
28. Singh, A., Van Hamme, J.D. Ward, O.P. (2007) Surfactants in microbiology and biotechnology: Part 2. Application aspects. Biotechnol Adv 25, 99 121.
29. Stestak, S. Ferkas, V. (2001) In situ assays of fungal enzymes in cells permeabilized by osmotic shock. Anal Biochem 292, 34 39.
30. Teertstra, W.R., Lugones, L.G. Wosten, H.A.B. (2004) In situ hybridization in filamentous fungi using peptide nucleic acid probes. Fungal Genet Biol 41, 1099 1103.
31. Valinsky, L., Della Vedova, G., Jiang, T. Borneman, J. (2002) Oligonucleotide fingerprinting of rRNA genes for analysis of fungal community composition. Appl Environ Microbiol 68, 5999 6004.
32. de Vos, M.M. Nelis, H.J. (2003) Detection of Aspergillus fumigatus hyphae by solid phase cytometry. J Microbiol Meth 55, 557 564.
33. Wallner, G., Amann, R. Beisker, W. (1993) Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytom Part A 14, 136 143.
34. van der Werf, M.J., Hartmans, S. van den Tweel, W.J.J. (1995) Permeabilization and lysis of Pseudomonas pseudoalcaligenes cells by Triton X-100 for efficient production of d-malate. Appl Microbiol Biotechnol 43, 590 594.
35. Wolken, W.A.M., Tramper, J. van der Werf, M.J. (2003) What can spores do for us? TRENDS in Biotechnol 21, 338 345.
36. Wu, Z., Tsumura, Y., Blomquist, G. Wang, X-R. (2003) 18S rRNA gene variation among common airborne fungi, and development of specific oligonucleotide probes for the detection of fungal isolates. Appl Environ Microbiol 69, 5389 5397.
37. Xie, G. West, T.P. (2006) Citric acid production by Aspergillus niger on wet corn distillers grains. Lett Appl Microbiol 43, 269 273.
38. Xufre, A., Albergaria, H., Inacio, J., Spencer-Martins, I. Girio, F. (2006) Application of fluorescence in situ hybridization (FISH) to the analysis of yeast population dynamic in winery and laboratory grape must fermentations. Int J Food Microbiol 108, 376 384.